【Objective】 This study aims to systematically investigate and optimize the key cultural conditions that regulate the induction and subsequent proliferation of embryogenic callus in Cryptomeria fortunei during somatic embryogenesis. The research focused on identifying specific parameters and factor interactions that significantly affect the efficiency of embryogenic tissue formation and growth. A further goal is to establish a robust, reproducible and efficient technical system for somatic embryogenesis in this species, to support large-scale clonal propagation, genetic improvement and germplasm conservation. The ultimate objective aims to develop a standardized protocol that enhances the induction rate and proliferation capacity of embryogenic callus while preserving its genetic stability and embryogenic potential. 【Method】Using immature seeds of C. fortunei as explants, we systematically analyzed the effects of several factors on the induction and proliferation of embryogenic callus. These factors included collection time, type of basal medium, type and concentration of carbon source, as well as the type, ratio and concentration of plant growth regulators (PGRs). 【Result】(1) After comprehensive screening of various basal media—including MS, 1/2 MS, DCR, 1/2 DCR, WPM and MLV—DCR medium was identified as the most effective, yielding the highest induction rate for embryogenic callus at 56.0%. (2) Significant differences in induction efficiency were observed among different PGR treatments. The optimal formulation consisted of DCR medium supplemented with 2.0 mg/L 2,4-D, 0.5 mg/L 6-BA, and 0.25 mg/L KT, which achieved an induction rate of 58.0%, significantly outperforming other combinations. (3) The type of carbon source also considerably influenced induction efficiency. Among those tested, sucrose at a concentration of 30 g/L yielded the best result, with an induction rate of 40.67%. (4) During the subculture phase for proliferation, the concentrations of activated carbon and sucrose, as well as the type and combination of PGRs, markedly affected proliferation efficiency. The optimal proliferation was achieved using DCR medium supplemented with 0.50 mg/L 6-BA, 1.0 mg/L 2,4-D, 2.0 g/L activated carbon, and 30 g/L sucrose. This combination resulted in a proliferation coefficient of 615.44 and completely suppressed browning throughout the culture period. 【Conclusion】This study demonstrates that multiple critical factors, including the developmental stage of zygotic embryos, choice of basal medium, type and concentration of carbon source, combination and concentration of PGRs, and amount of activated carbon, significantly influence the induction and proliferation of embryogenic callus in C. fortunei. By systematically optimizing cultural conditions, a stable and efficient somatic embryogenesis system has been successfully established. This system provides a reliable technical foundation for rapid propagation of superior germplasm resources and supports genetic transformation programs for C. fortunei in China. The established protocol yields reproducible results and holds significant value for conservation and genetic improvement efforts.