Identification and expression analysis of the Eucommia EuWRI1 gene family

CHENG Jiaqi, DU Ping, LI Zhiguo, YANG Na, REN Xiaoyue, LU Wenjie, WANG Yan

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0 DOI: 10.12302/j.issn.1000-2006.202508007

Identification and expression analysis of the Eucommia EuWRI1 gene family

  • CHENG Jiaqi1, DU Ping2, LI Zhiguo2, YANG Na1, REN Xiaoyue1, LU Wenjie1,*, WANG Yan2,*
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Abstract

【Objective】WRI1 is a key transcription factor regulating plant oil biosynthesis. Conducting a genome-wide identification and bioinformatics analysis of EuWRI1 in Eucommia ulmoides can provide a theoretical reference for subsequent studies on the mechanism by which EuWRI1 genes influence oil accumulation in Eucommia ulmoides. 【Method】Bioinformatics methods were used to systematically analyze the EuWRI1 gene family members in Eucommia ulmoides, and qRT-PCR was conducted to analyze their expression patterns in different tissues and at various stages of seed development. 【Result】A total of 23 EuWRI1 members containing two AP2 conserved domains ( EuWRI1-1 to EuWRI1-23) were identified, divided into four subfamilies. The physicochemical properties of the EuWRI1 family vary significantly, but the gene structures and protein sequences are highly conserved. The 23 members are randomly distributed on nine chromosomes, mainly located in the nucleus, containing two segmental duplication events, and showing higher homology with oilseed crops. The promoters contain elements related to MYB, MYC, and light responses, and the expression of EuWRI1 family genes exhibits tissue- and temporal-specificity. 【Conclusion】This study performed a genome-wide identification of EuWRI1 family members, clarified their basic characteristics and expression patterns, and identified EuWRI1-9 as a potential key transcription factor regulating seed oil synthesis, providing a reference for further elucidating the molecular mechanisms and biological functions of Eucommia oil accumulation.

Key words

Eucommia ulmoides / Seed kernel / Oil / EuWRI1 / Gene expression

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CHENG Jiaqi, DU Ping, LI Zhiguo, YANG Na, REN Xiaoyue, LU Wenjie, WANG Yan. Identification and expression analysis of the Eucommia EuWRI1 gene family[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 0 https://doi.org/10.12302/j.issn.1000-2006.202508007

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