Leaf epidermal micromorphological characteristics and taxonomic significance of 11 species and 4 varieties in Stachyurus

Ren Zhiqin, Huang Anling, Jiang Jinxiang, Yang Rong, Xu Chaojuan, Li Shuang, Wang Zhiwei

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0 DOI: 10.12302/j.issn.1000-2006.202512007

Leaf epidermal micromorphological characteristics and taxonomic significance of 11 species and 4 varieties in Stachyurus

  • Ren Zhiqin, Huang Anling, Jiang Jinxiang, Yang Rong, Xu Chaojuan, Li Shuang, Wang Zhiwei*
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Abstract

【Objective】The objective of this study was to investigate the leaf epidermal micromorphological characteristics and their taxonomic identification value in Stachyurus. 【Method】The leaf epidermal micromorphological characteristics of 11 species and 4 varieties in this genus were observed using optical microscopy. Key taxonomic identification indices were selected based on the Minimum Data Set (MDS), providing more criteria for species taxonomic identification of the genus. 【Result】Stomata were exclusively distributed in Stachyurus species on the lower epidermis and were all anisocytic. However, the anticlinal wall patterns of upper and lower epidermal cells showed significant differences, which could be classified into three types: nearly straight, slightly sinuous, and sinuous. Only S. retusus had non-glandular trichomes on the lower epidermis. Five key identification indices were selected based on the MDS, namely the length-width ratio of upper epidermal cell, upper epidermal cell area, upper epidermal cell density, stomatal apparatus length, and length-width ratio of stomatal apparatus. Their numerical ranges were 1.40-1.86, 669.60-2178.47 μm², 521-1343 mm⁻², 13.71-26.56 μm, and 1.22-1.59, respectively. Among the five indices, the stomatal apparatus length showed the highest number of significant differences (76), followed by upper epidermal cell area and upper epidermal cell density (both 74), length-width ratio of stomatal apparatus (45), and length-width ratio of upper epidermal cell (41). Common closely related species (or varieties), such as S. retusus and S. szechuanensis, S. yunnanensis, S. oblongifolius, S. callosus and S. obovatus, as well as S. salicifolius and its variety S. salicifolius var. lancifolius (etc.) could be effectively distinguished by combining with the anticlinal wall patterns, trichome characteristics, and the five key indices. However, leaf epidermal micromorphological characteristics have limited significance in infrageneric classification for this genus. 【Conclusion】Leaf epidermal micromorphological characteristics hold significant value for species identification in Stachyurus, and can provide more evidence for its taxonomic revision.

Key words

Stachyurus / leaf epidermal micromorphological characteristics / minimum data set(MDS) / taxonomic identification

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Ren Zhiqin, Huang Anling, Jiang Jinxiang, Yang Rong, Xu Chaojuan, Li Shuang, Wang Zhiwei. Leaf epidermal micromorphological characteristics and taxonomic significance of 11 species and 4 varieties in Stachyurus[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 0 https://doi.org/10.12302/j.issn.1000-2006.202512007

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