Effects of shelf life on growth promotion and disease resistance of Bacillus velezensis YH-18 inoculant in peach seedlings

LIU Kuiying, SHI Huimin, TIAN Wenbai, YE Jianren

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0 DOI: 10.12302/j.issn.1000-2006.202512011

Effects of shelf life on growth promotion and disease resistance of Bacillus velezensis YH-18 inoculant in peach seedlings

  • LIU Kuiying, SHI Huimin, TIAN Wenbai, YE Jianren*
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Abstract

【Objective】 This study investigates the effects of shelf life on the quality , growth-promoting and disease-control efficacy of Bacillus velezensis YH-18 inoculant, aiming to provide a scientific basis for ensuring its effectiveness and for its popularization and application.【Method】YH-18 inoculants with different shelf lives ( 0, 0.5, 1, 1.5, 2 a ) were used as test materials. The viable counts of YH-18 inoculants and their antagonistic activity against pathogenic bacteria were determined by the dilution plating method and the plate confrontation method. The contents of K, Ga and Mg in the inoculants at different shelf lives were measured by flame emission spectrometry and atomic absorption spectrometry. The total sugar content of YH-18 inoculants at different shelf lives was determined by the 3,5-dinitrosalicylic acid method. In pot experiments, growth indices of peach seedlings, including plant height, ground diameter, chlorophyll content, and leaf area, were measured after treatment with inoculants of different shelf lives. The disease incidence, nodule number and diameter, disease index, and control efficacy were calculated.【Result】When the shelf life of YH-18 inoculant was 0.5 a and 1 a, the number of effective viable bacteria remained in the same order of magnitude as that of the initial inoculant. In contrast, the effective viable count of YH-18 inoculant with a shelf life of 1.5 a and 2 a decreased by one order of magnitude compared with that of the initial inoculant. In the plate confrontation assay, the antagonistic ability of inoculants stored for 1 a and beyond was significantly lower than that of the initial inoculant. The antagonistic ability of the bacterial suspension prepared from inoculants stored for 1.5 a and 2 a was also significantly lower than that of the initial inoculant. Furthermore, the sterile fermentation filtrate prepared from inoculants stored for 0.5 a and beyond completely lost its antagonistic activity. Shelf life had no significant effect on the contents of inorganic elements in the inoculant. However, the total sugar content of inoculants stored for 1 a and beyond was significantly lower than that of the initial inoculant. Pot experiments showed that YH-18 inoculants with different shelf lives all promoted increases in plant height, ground diameter, chlorophyll content, and leaf area of peach seedlings. The growth-promoting effect decreased with prolonged shelf life, but no significant difference was observed compared with the initial inoculant. The control efficacy against crown gall disease decreased with extended shelf life. Compared with the initial inoculant, the preventive efficacy of inoculants stored for 0.5 a and 1 a decreased to 53.33% and 46.67%, respectively, from 80.00% for the initial inoculant, respectively, indicating a relatively small reduction. In contrast, the preventive efficacy of inoculants stored for 1.5 a and 2 a dropped to 19.99% and 10.00%, respectively, from 80.00% for the initial inoculant.【Conclusion】The recommended shelf life of YH-18 inoculant is within 1 year, which can substantially maintain its growth-promoting and disease-control efficacy.

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shelf life / Bacillus velezensis YH-18 / bacterial agent quality / crown gall disease / growth promotion and disease resistance

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LIU Kuiying, SHI Huimin, TIAN Wenbai, YE Jianren. Effects of shelf life on growth promotion and disease resistance of Bacillus velezensis YH-18 inoculant in peach seedlings[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 0 https://doi.org/10.12302/j.issn.1000-2006.202512011

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