Identification and Expression Analysis of HAK5 Gene Subfamily in Nitraria sibirica Pall.

Xu Guoxuan, Zhang Huilong, Li Rong, Duan Rongfeng, Luo Zhibin, Yang Xiuyan

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 0 DOI: 10.12302/j.issn.1000-2006.202512030

Identification and Expression Analysis of HAK5 Gene Subfamily in Nitraria sibirica Pall.

  • Xu Guoxuan1,2, Zhang Huilong1,2, Li Rong1,2, Duan Rongfeng1,2, Luo Zhibin1,2,*, Yang Xiuyan1,2,*
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Abstract

【Objective】To identify HAK5 subfamily members in Nitraria sibirica Pall. and analyze the expression patterns of NsHAK5s under NaCl treatments. 【Method】NsHAK5 subfamily members were analyzed using bioinformatics methods with software such as TBtools. Experiments were conducted on 50-day-old, healthy, and uniformly growing clonal plants. The test seedlings were hydroponically cultured in 1/2 Hoagland nutrient solution supplemented with 0 mM, 200 mM, and 400 mM NaCl. After 7 and 14 days of salt treatment, root, stem, and leaf tissue samples were collected from the plants. The tissue-specific expression patterns and salt stress-responsive expression characteristics of the NsHAK5 subfamily members were analyzed using RT-qPCR.【Results】Six HAK5s were identified in the genome of the N. sibirica Pall., which were distributed on the second and fourth chromosomes. Phylogenetic analysis revealed that the NsHAK5 subfamily members were segregated into two distinct clades. Analysis of cis-acting elements indicated that the promoter regions of NsHAK5s contained multiple elements which were associated with light, hormone and abiotic stress responses. The expression of NsHAK5s exhibited a tissue-specific pattern: NsHAK5c, NsHAK5d, NsHAK5e, and NsHAK5f were specifically expressed in the roots, while NsHAK5a and NsHAK5b were specifically expressed in the leaves. Under salt exposure condition, the expression levels of NsHAK5c, NsHAK5d, and NsHAK5e were significantly upregulated in the roots, the expression levels of NsHAK5a and NsHAK5b were increased in the leaves, but the expression level of NsHAK5f was significantly downregulated in all of the tissues in comparison with the expression levels of these genes in the plants treated without salinity.【Conclusion】Six NsHAK5s were identified in the genome of N. sibirica. Among them, five members exhibited upregulated expression under salinity, while one member showed the down-regulation. The tissue-specific expression patterns of NsHAK5s suggest a potential functional divergence among the members, indicating these members of N. sibirica may play distinct roles in responding to salinity. These findings provide insights for elucidating the molecular mechanisms of NsHAK5s underlying salt tolerance of N. sibirica.

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Nitraria sibirica Pall. / HAK5 / salt stress / gene subfamily

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Xu Guoxuan, Zhang Huilong, Li Rong, Duan Rongfeng, Luo Zhibin, Yang Xiuyan. Identification and Expression Analysis of HAK5 Gene Subfamily in Nitraria sibirica Pall.[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 0 https://doi.org/10.12302/j.issn.1000-2006.202512030

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