Variation of EST-SSR molecular markers among provenances of Chinese fir

XU Yang,CHEN Jinhui, ZHAO Yaqi, WANG Ying, WANG Xinmin, LIU Weidong, SHI Jisen

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (01) : 1-8.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (01) : 1-8. DOI: 10.3969/j.issn.1000-2006.2014.01.001

Variation of EST-SSR molecular markers among provenances of Chinese fir

  • XU Yang1,CHEN Jinhui1, ZHAO Yaqi1, WANG Ying1, WANG Xinmin1, LIU Weidong1, SHI Jisen1*
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Abstract

In order to detect the genetic variation of Chinese fir provenance, 42 Chinese fir provenance samples were selected for genetic diversity analysis based on EST-SSR makers. Experimental results showed that there was higher genetic polymorphism among Chinese fir provenance. Base on the 17 locus amplified by the 15 EST-SSR pair primers, a total of 52 polymorphic alleles with average of 3.058 8 alleles for per locus were found. The average PIC was 0.296 for each locus. Comparing to others makers, more genetic variation information could be detected by SSR. Except provenance No. 2 and provenance No. 16, the other 40 provenances could be classified into four groups, with genetic distance of 10 as threshold. Our results fit well with the formal research on Chinese fir seed zones map based on growth and adaptive traits.In this study, we also found that there were some SSR variations located in the coding area of functional genes responding to certain biological or non-biological stress. The authors suggested that the geographic variation of Chinese fir might be due to these allelic variations of genes or in other words, the adaptive variation and local fixation.

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XU Yang,CHEN Jinhui, ZHAO Yaqi, WANG Ying, WANG Xinmin, LIU Weidong, SHI Jisen. Variation of EST-SSR molecular markers among provenances of Chinese fir[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(01): 1-8 https://doi.org/10.3969/j.issn.1000-2006.2014.01.001

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