Optimization of fermentation medium and culture condition of a thermostable protease from moderate thermophilic strain Bacillus subtilis BY25

ZHU Hong, WANG Yiming,LIN Xiangui

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (01) : 31-35.

PDF(1603073 KB)
南京林业大学学报（自然科学版）
PDF(1603073 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (01) : 31-35. DOI: 10.3969/j.issn.1000-2006.2014.01.006

Optimization of fermentation medium and culture condition of a thermostable protease from moderate thermophilic strain Bacillus subtilis BY25

  • ZHU Hong1,2,3, WANG Yiming1,LIN Xiangui1*
Author information +
History +

Abstract

The fermentation media components and culture conditions of the thermostable protease from a thermophilic strain Bacillus subtilis BY25 were further studied. A six factor and three level orthogonal experiment was conducted to observe the effects of fermentation medium components on thermostable protease yield. Wheat bran was replaced by soybean cake powder for its low contribution rate. Single factor experiment was carried out to optimize the protease production. The optimized culture condition was at 30 ℃, with initiating pH of 8.0, 4.5%-5% inoculum size for 72 h; and the optimized fermentation medium components were described as follows:glucose 60 g/L, soybean cake powder 60 g/L, Na2HPO4 4 g/L and CaCl2 0.5 g/L. After fermentation, the casein hydrolysis activity of the enzyme was estimated up to 101.1 μmol/(min·mL), much higher than other domestic reports.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
ZHU Hong, WANG Yiming,LIN Xiangui. Optimization of fermentation medium and culture condition of a thermostable protease from moderate thermophilic strain Bacillus subtilis BY25[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(01): 31-35 https://doi.org/10.3969/j.issn.1000-2006.2014.01.006

References

[1] Sana B, Ghosh D, Saha M,et al. Purification and characterization of a salt, solvent, detergent and bleach tolerant protease from a new gamma-proteobacterium isolated from the marine environment of the sundarbans [J]. Process Biochem, 2006, 41(1):208-215.
[2] Yagasaki M,Hashimoto S. Syntesis and application of dipeptides; current status and perspectives[J]. Appl Microbiol Biot, 2008, 81(1):13-22.
[3] Madingan M T, Martinko J M, Parke J. 微生物生物学 [M]. 杨文博,译.北京:科学出版社,2001.
[4] 彭素萍, 林先贵, 王一明. 一株产高温蛋白酶耐热菌BY25的产酶条件与酶学性质研究[J]. 土壤, 2010, 42(3):410-414. Peng S P, Lin X G, Wang Y M. Fermentation condition and some properities of a thermostable protease from moderate thermophilic strain BY25[J]. Soils, 2010, 42(3):410-414.
[5] 戴玄, 唐兵, 陈向东, 等. 产高温蛋白酶微生物菌种资源的研究[J]. 微生物学杂志, 1997, 17(3):25-29. Dai X, Tang B, Chen X D,et al. Study on microbial strain resources for thermophilic protease production[J]. Journal of Microbiology, 1997, 17(3):25-29.
[6] Heussen C,Dowdle E B. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates [J]. Anal Biochem, 1980, 102(1):196-202.
[7] Wilson E D. Studies in bacterial proteases I. The relation of protease production to the culture medium[J]. J Bacteriol, 1930, 20(1):41-59.
[8] Semets E V, Glenn A R, May B K, et al. Accumulation of messenger ribonucleic acid specific for extracellular protease in Bacillus subtilis 168 [J]. J Bacteriol, 1973, 116(2):531-534.
[9] Fisher S H. Regulation of nitrogen metabolism in Bacillus subtilis:vive la difference [J]. Mol Microbiol, 1999, 32(2):223-232.
[10] 活泼. 高温中性蛋白酶及其产生菌的初步研究[J]. 工业微生物, 2003, 33(2):30-34. Huo P. Preliminary studies on the thermostable neutral protease[J]. Industrial Microbiology, 33(2):30-34.
[11] 舒丹,李宏,严建华, 等. 高温芽孢杆菌碱性蛋白酶发酵条件及酶性质研究[J]. 四川大学学报, 2004, 41(4):856-860. Shu D, Li H, Yan J H, et al. Study on fermentation condition and properity of alkaline protease from thermoliphic Bacillus sp. SD-142[J]. Journal of Sichuan University, 2004, 41(4):856-860.
[12] 朱泓, 王一明, 林先贵, 等. 一株产高温蛋白酶耐热菌BY25的产酶条件与酶学性质研究[J]. 土壤, 2013, 45(5):899-904. Zhu H, Wang Y M, Lin X G, et al. Differences in enzyme production induced by fermentation media of a moderate thermophilic strain BY25[J]. Soils, 2013, 45(5):899-904.
[13] 朱泓, 王一明, 林先贵. 一株产高温蛋白酶高产菌株菌体增殖和产酶关系的响应面模型研究[J]. 微生物学通报, 2013-10-11,http://www.cnki.net/kems/detail/11.1996.Q.20131011.2259.039.html. Zhu H, Wang Y M, Lin X G. RSM modeling research of the relationship between cell growth and protease yield of a a moderate thermophilic strain Bacillus subtilis BY25 [J]. Microbiology, 2013-10-11,http://www.cnki.net/kems/detail/11.1996.Q.20131011.2259.039.html.
[14] Tokunaga T, Rashid M H, Kuroda A, et al. Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor and autolysin operon of Bacillus subtilis [J]. J Bacteriol, 1994, 176(16):5177-5180.
[15] Dod B, Balassa G, Raulet E, et al. Spore control(Sco)mutations in Bacillus subtilis[J]. Molecular and General Genetics, 1978, 163(1):45-56.
[16] Ogura M,Tanaka T. Transcription of Bacillus subtilis degR is σ D-dependent and suppressed by multicopy proB through σD [J]. J Bacteriol, 1996, 178:216-222.
[17] Perego M,Hoch J A. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis [J]. J Bacteriol, 1988, 170:2560-2567.
PDF(1603073 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉