Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (03) : 34-38.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (03) : 34-38. DOI: 10.3969/j.issn.1000-2006.2015.03.007

Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

  • MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian*
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Abstract

To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high-throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average density of one SSR per 3.49 kb. Tri-nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs,(AG/CT)n was the most frequent repeat motif(18.34%), followed by the(AAG/CTT)n(12.42%). The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation(P<0.05)between the frequency of microsatellites and the length, the correlation coefficient was -0.246.

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MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian. Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(03): 34-38 https://doi.org/10.3969/j.issn.1000-2006.2015.03.007

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