Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

doi:10.3969/j.issn.1000-2006.2015.03.007

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (03): 34-38.doi: 10.3969/j.issn.1000-2006.2015.03.007

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Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian^*

Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University, Nanjing 210037, China

Online:2015-05-30 Published:2015-05-30

Abstract

Abstract: To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high-throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average density of one SSR per 3.49 kb. Tri-nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs,(AG/CT)_n was the most frequent repeat motif(18.34%), followed by the(AAG/CTT)_n(12.42%). The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation(P<0.05)between the frequency of microsatellites and the length, the correlation coefficient was -0.246.

CLC Number:

Q948
Q78

MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian. Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(03): 34-38.

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Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

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