Abstract: The adsorption and desorption of enzymes on the substrates are critical for bioconversion of cellulosic materials. QCM-D(quartz crystal microbalance with dissipation), different from traditional chemical analysis methods, could reflect the whole dynamic process and the features of kinetics of enzymatic hydrolysis online. QCM-D was a surface-sensitive analytical technique based on the piezoelectric properties of quartz crystal and could sense the mass change in nanoscale. In this review, the operating principle of QCM-D and the preparation methods of the biosensor, as well as the latest developments of the application of QCM-D on the research of enzymatic hydrolysis of cellulosic materials were introduced. Generally, QCM-D gives the information in situ and responses to the surface quality changes on the substrates on top of quartz crystal in real-time through the variations of frequency and dissipation. Thus the adsorption and desorption of enzymes on the substrate, and the dynamic enzymatic hydrolysis could be visually monitored and analyzed. In addition, the relationships between frequency and dissipation obtained from QCM-D reflect the structure information of adsorbed layers. However, there still were part of limitations in enzymatic hydrolysis process. For instance, relatively high demands of film-formation of substrates were needed when using QCM-D. Besides, further researches were required in the aspect of quantitative analysis.