Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt.

doi:10.3969/j.issn.1000-2006.201512021

ZHANG Huihui, LIU Longyun, WU Caie, LI Tingting, FAN Gongjian, YING Ruifeng

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (06) : 47-53.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (06) : 47-53. DOI: 10.3969/j.issn.1000-2006.201512021

Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt.

ZHANG Huihui^1,2, LIU Longyun^1,2, WU Caie^1,3*, LI Tingting^1,3, FAN Gongjian^1,3, YING Ruifeng^1,3

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Abstract

【Objective】The optimal conditions for the semi-bionic extraction(SBE)of flavonoids from Rosa roxburghii Tratt. were identified. 【Method】Response surface methodology(RSM)was carried out to investigate the effects of ethanol concentration, extraction temperature, solid-liquid ratio, and extraction time on the yield of flavonoids from Rosa roxburghii Tratt. 【Result】The results showed that the optimal conditions for SBE were 80% ethanol, solid-to-liquid ratio of 1:13.2(mass volume ratio), and extraction at 90 ℃ for 80-40-40 min; the extraction yield of flavonoids under these conditions was 27.70 mg/g. The antioxidant activity was significantly higher than that in the case of Tween auxiliary extraction and ultrasonic-assisted extraction. Component I and Component II were separated by semi-preparing liquid chromatography, and their retention times agreed with those of quercetin and kaempferol. 【Conclusion】SBE is superior to the traditional ethanol extraction, and in combination with RSM, offers better yields of purified quercetin and kaempferol.

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ZHANG Huihui, LIU Longyun, WU Caie, LI Tingting, FAN Gongjian, YING Ruifeng. Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(06): 47-53 https://doi.org/10.3969/j.issn.1000-2006.201512021

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