Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.

HUANG Lin, YANG Jiyun, LI Qiucheng, ZHANG Ya, YE Jianren

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (05) : 175-179.

PDF(3564776 KB)
PDF(3564776 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (05) : 175-179. DOI: 10.3969/j.issn.1000-2006.201601004

Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.

  • HUANG Lin, YANG Jiyun, LI Qiucheng, ZHANG Ya, YE Jianren*
Author information +
History +

Abstract

【Objective】 Identify the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort. 【Methods】 The method of tissue isolation was used to screen for suspected pathogens of stem canker on S. japonica Linn. var. japonica f. pendula Hort. A field experiment was performed to confirm the stem canker pathogen. Morphological characters, rDNA-ITS and EF-α gene sequence analyses were used to identify the pathogen causing this disease. 【Result】 A Koch's postulates assay was performed on the F6 isolate, and this fungal strain was identified as Fusarium solani species complex(FSSC).【Conclusion】F. solani species complex(FSSC)was confirmed as the pathogen of stem canker on S. japonica Linn. var. japonica f. pendula Hort. This is the first study describing the pathogen causing stem canker on S. japonica Linn. var. japonica f. pendula Hort.

Key words

Keywords:stem canker on Sophora japonica Linn. var. japonica f. pendula Hort. / Fusarium solani species complex(FSSC) / symptom / morphological character / rDNA-ITS / EF-α

Cite this article

Download Citations
HUANG Lin, YANG Jiyun, LI Qiucheng, ZHANG Ya, YE Jianren. Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(05): 175-179 https://doi.org/10.3969/j.issn.1000-2006.201601004

References

[1] SHIDA H, UMINO T, TSUJI K, et al. Studies on the anti-hemorrhagic substances in herbs classified as hemostatics in Chinese medicine. X: on hemostatic activities of the parched herbs for hemostatics[J]. Yakugaku Zasshi, 1989, 109: 79.
[2] TANG Y, LOU F, WANG J, et al. Four new isoflavone triglycosides from Sophora japonica[J]. Journal of Natural Products, 2001, 64: 1107-1110.
[3] KIM J M, YUN-CHOI H S. Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica[J]. Archives of Pharmacal Research, 2008, 31(7):886-890.DOI:10.1007/s12272-001-1242-1.
[4] 杨旺. 森林病理学[M]. 北京:中国林业出版社, 1996:147-148.
[5] 陈守常, 朱天辉, 杨佐忠. 四川森林病害[M]. 成都:四川科学技术出版社,2006: 473.
[6] 方中达.植病研究方法[M]. 3版. 北京:中国农业出版社,1998:122-124.
[7] HOU Z, XUE C, PENG Y, et al. A mitogen-activated protein kinase gene(MGV1)in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection[J]. Molecular Plant-Microbe Interactions, 2002, 15: 1119-1127.
[8] 许志刚. 普通植物病理学[M]. 4版. 北京:中国农业出版社,2003:23-25.
[9] 陈剑山. 来自海南岛的镰刀菌的种类鉴定[D]. 儋州: 华南热带农业大学, 2007. CHEN J S. The identification of the Fusarium spp. from Hainan Island [D]. Danzhou:South China University of Tropical Agriculture, 2007.
[10] LESLIE J F, SUMMERELL B A, BULLOCK S. The Fusarium laboratory manual[M]. Iowa: Wiley-Blackwell, 2006: 250-255.
[11] DAMM U, MOSTERT L, CROUSS P W, et al. Novel Phaeoacremonium species associated with necrotic wood of Prunus trees[J]. Persoonia, 2008, 20:87-102.DOI:10.3767/003158508X324227.
[12] GARDES M, BRUNS T D. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J]. Molecular Ecology, 1993, 2:113-118.
[13] WHITE T J, BRUNS T, LEE J, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//PCR protocols: a guide to methods and applications. San Diego, California:Academic Press, 1990:315-322.
[14] O'DONNELL K, KISTLER HC, CIGELNIK E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogis[J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95:2044-2049.
[15] HUANG L, LI Q C, ZHANG Y, et al. Colletotrichum gloeosporioides sensu strict is a pathogen of leaf anthracnose on evergreen spindle tree(Euonymus japonicus)[J]. Plant Disease, 2016, 100:672-678.
[16] ZHANG N, O'DONNELL K, SUTTON D A, et al. Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment[J]. Clinical Microbiology, 2006, 44(6): 2186-2190.
[17] O'DONNELL K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex[J]. Mycologia, 2000, 92:919-938.
PDF(3564776 KB)

Accesses

Citation

Detail

Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

/