Transcriptome sequencing, de novo assembly and expression analysis of several genes related to internode development in Pseudosasa japonica

WEI Qiang, DING Yulong

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (05) : 42-48.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (05) : 42-48. DOI: 10.3969/j.issn.1000-2006.201601006

Transcriptome sequencing, de novo assembly and expression analysis of several genes related to internode development in Pseudosasa japonica

  • WEI Qiang, DING Yulong
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Abstract

【Objective】Describe the molecular basis of bamboo rhizome development. 【Methods】 We used next-generation sequencing technology and qRT-PCR to analyze the transcriptome profile of the underground stems of Pseudosasa japonica and the expression pattern of genes related to internode elongation, respectively. 【Results】 A total of 119 767 unigenes, with an average length of 1 008 bp were determined, 63.8% of which had annotated results. A total of 16 254 unigenes were annotated to 137 KEGG(kyo to Encyclopedia of Genes and Genomes)pathways. MapMan software identified 1 864 transcription factors, 603 hormone related genes, and 564 cell-wall building related genes, including 92 unigenes related to lignin biosynthesis. The qRT-PCR analysis showed that, among the nine genes used for detecting, gibberellin-related genes had the highest expression level in the elongation initiation stage of internode development, whereas cell wall growth- and cytoskeleton-related genes had the strongest expression in the rapid elongation of internodes. 【Conclusion】 These results illustrate the molecular basis for rhizome development in Ps. japonica. The molecular mechanism underlying the development of rhizomes is a complex network consisting of hormones, transcription factors and downstream functional genes.

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WEI Qiang, DING Yulong. Transcriptome sequencing, de novo assembly and expression analysis of several genes related to internode development in Pseudosasa japonica[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(05): 42-48 https://doi.org/10.3969/j.issn.1000-2006.201601006

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