The screening and identification of two bacterial strains with nematicidal activity against Bursaphelenchus xylophilus

LI Liangliang, TAN Jiajin, CHEN Fengmao

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (04) : 37-41.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (04) : 37-41. DOI: 10.3969/j.issn.1000-2006.201601057

The screening and identification of two bacterial strains with nematicidal activity against Bursaphelenchus xylophilus

  • LI Liangliang, TAN Jiajin*, CHEN Fengmao
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Abstract

【Objective】The aim of the present study was to screen bacteria with nematicidal activity against the pine wood nematode(Bursaphelenchus xylophilus). 【Method】A total of 137 bacteria strains, which were isolated from the stems of pine trees in Nanjing, Luoyang and Shanghai, China, were assessed for nematicidal activity by immersion test with cultural filtrate and buffer suspension. 【Result】Cultural filtrates of three strains had stronger nematicidal activity than the other strains, and nematodes treated with these strains reached 100% mortality after 48 h and even exhibited decomposition when treated with the cultural filtrate of strain LYMC-3. The cultural filtrates of three strains, which were diluted 2, 4 and 10 times still showed high nematicidal activity. Nematicidal activity decreased with increasing dilution, and when nematodes were treated with 10× diluted filtrates for 48 h, the greatest observed mortality was 94.7% with strain LYMC-3. Moreover,the buffer suspension of strain NJSZ-13 had strong nematicidal activity against B. xylophilus, and 81.5% nematode mortality was achieved after 48 h treatment with 105 cfu/mL buffer suspension of strain NJSZ-13. Based on morphology, physiological and biochemical characteristics, Biolog system identification, 16SrDNA sequences, and a phylogenetic tree, the strains LYMC-3 and NJSZ-13 were identified as Bacillus pumilus and B. cereus, respectively.【Conclusion】Two strains of bacteria LYMC-3 and NJSZ-13 with high nematicidal activity which were isolated from the stems of pine trees could provide potential solution for biological control of Bursaphelenchus xylophilus.

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LI Liangliang, TAN Jiajin, CHEN Fengmao. The screening and identification of two bacterial strains with nematicidal activity against Bursaphelenchus xylophilus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(04): 37-41 https://doi.org/10.3969/j.issn.1000-2006.201601057

References

[1] ZHAO B G, FUTAI K, SUTHERLAND J R, et al.Pine wilt disease[M].Tokyo: Springer, 2008.
[2] RYSS A Y, KULINICH O A, SUTHERLAND J R.Pine wilt disease: a short review of worldwide research[J].Forestry Studies in China, 2011, 13(2): 132-138.DOI: 10.1007/s11632-011-0205-8.
[3] DONG J Y, LI X P, LI L, et al. Preliminary results on nematicidal activity from culture filtrates of Basidiomycetes against the pine wood nematode Bursaphelenchus xylophilus(Aphelenchoididae)[J].Annals of Microbiology, 2006, 56(2): 163-166.DOI: 10.1007/BF03174999.
[4] 李小平, 吴如其, 夏民洲, 等.苦豆碱、脱氢苦豆碱对松材线虫的毒性与结构的关系[J].南京林业大学学报(自然科学版), 2000, 24(4): 78-81.DOI: 10.3969/j.issn.1000-2006.2000.04.019. LI X P, WU R Q, XIA M Z, et al.Relationship between molecular structure and nematicidal activity of two alkaloids, aloperine and Δ11-dehydroaloperine[J].Journal of Nanjing Forestry University(Natural Sciences Edition), 2000, 24(4): 78-81.
[5] 丹阳, 黄忠良, 魏孝义,等.松材线虫的天然毒素研究进展[J].热带亚热带植物学报, 2004, 12(4): 381-387.DOI: 10.3969/j.issn.1005-3395.2004.4.018. DAN Y, HUANG Z L, WEI X Y, et al.Progress in study on natural toxins against Bursaphelenchus xylophilus[J].Journalof Tropical and Subtropical Botany,2004, 12(4): 381-387.
[6] 董锦艳, 李铷, 张克勤.松材线虫生物防治研究进展[J].植物保护, 2005, 31(5): 9-15.DOI: 10.3969/j.issn.0529-1542.2005.05.002. DONG J Y, LI R, ZHANG K Q.Advances in biological control of Bursaphelenchus xylophilus by natural enemies and nematocidal compounds from plants[J].Plant Protection, 2005, 31(5): 9-15.
[7] 汪来发.根结线虫生物防治研究进展[J].南京林业大学学报(自然科学版), 2002, 26(1): 64-68.DOI: 10.3969/j.issn.1000-2006.2002.01.016. WANG L F.A review of biological control of root-knot nematodes[J].Journal of Nanjing Forestry University(Natural Sciences Edition), 2002, 26(1): 64-68.
[8] 茆振川.防治根结线虫的原核生防菌的筛选及作用[D].杨凌:西北农林科技大学, 2004. MAO Z C.Study on screening biocontrol agents from prokaryotic organisms for control of Myloidogyne spp.[D]. Yangling: Northwest Sci-Tech University of Agriculture and Forestry, 2004.
[9] OKA Y, CHET I, SPIEGEL Y.Control of the root-knot nematode Meloidogyne javanica by Bacillus cereus[J].Biocontrol Science and Technology, 1993: 3(2): 115-126.DOI: 10.1080/09583159309355267.
[10] 余子全, 周燚, 孙明, 等.苏云金芽孢杆菌防治植物寄生线虫的研究进展[J].植物保护学报, 2004, 31(4): 420-424.DOI: 10.3321/j.issn:0577-7518.2004.04.015. YU Z Q, ZHOU Y, SUN M, et al.Progress of researches on activity of Bacillus thuringiensis against plant-parasitic nematodes[J].Journal of Plant Protection, 2004, 31(4): 420-424.
[11] 李长松. 拮抗细菌生物防治植物土传病害的研究进展[J]. 生物防治通报, 1992, 8(4): 168-172. DOI: 10.16409/j.cnki.2095-039x.1992.04.010. LI C S. A review on research progress of the biological control of soil-borne plant diseases with bacteria[J]. Chinese Journal of Biological Control, 1992, 8(4):168-172.
[12] 宋大新, 范长胜, 徐德强, 等.微生物学实验技术教程[M].上海: 复旦大学出版社, 1999.
[13] 东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京: 科学出版社, 2001.
[14] 布坎南R E, 吉本斯N E.伯杰细菌鉴定手册[M].北京: 科学出版社, 1984.
[15] 程池, 杨梅, 李金霞, 等.Biolog 微生物自动分析系统—细菌鉴定操作规程的研究[J].食品与发酵工艺, 2006, 32(5): 50-55.DOI: 10.3321/j.issn:0253-990X.2006.05.013. CHENG C, YANG M, LI J X, et al.Biolog microbial identification system:Study on the operating regulation of bacteria identification[J].Food and Fermentation Industries, 2006, 32(5): 50-55.
[16] 史亚萍, 张宏, 郭道森.一种促进蓝藻生长的海洋细菌的分离与鉴定[J].青岛大学学报(工程技术版), 2010, 25(2): 41-46.DOI: 10.3969/j.issn.1006-9798.2010.02.010. SHI Y P, ZHANG H, GUO D S. Isolation and identification of a bacterium strain promoting the growth of cyanobacterium[J].Journal of Qingdao University(Engineering and Technology Edition), 2010, 25(2): 41-46.
[17] 朱丽梅, 吴小芹, 徐旭凌.松材线虫拮抗细菌的筛选和鉴定[J].南京林业大学学报(自然科学版), 2008, 32(3): 91-94.DOI: 10.3969/j.issn.1000-2006.2008.03.022. ZHU L M, WU X Q, XU X L.The screening and identification of the bacterium with nematicidal activity to Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(3): 91-94.
[18] 牛秋红, 董冰雪, 黄思良, 等.松材线虫生防细菌的筛选、鉴定及其毒性因子的初步研究[J].中国生物工程杂志, 2010, 30(8): 76-81. NIU Q H, DONG B X, HUANG S L, et al.Screening, identification and virulence factor determination of the bacteria with nematicidal activity to Bursaphelenchus xylophilus[J].China Biotechnology, 2010, 30(8): 76-81.
[19] HUANG X W, NIU Q H, ZHOU W, et al. Bacillus nematocida sp.nov., a novel bacterial strain with nematotoxic activity isolated from soil in Yunnan in China[J], Systematic and Applied Microbiology, 2005, 28: 323-327.DOI: 10.1016/j.syapm.2005.01.008.
[20] 田宝玉, 黄薇, 柯崇榕.线虫生防细菌W3对线虫侵染活性的测定[J].生物技术, 2009, 19(2): 62-65. TIAN B Y, HUANG W, KE C R.Studying on the nematicidal and cuticle: degrading activities of a bacterium W3 strain[J].Biotechnology, 2009, 19(2): 62-65.
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