Abstract: 【Objective】Investigate the ability of strain SH16 to dissolve insoluble phosphate and its culture conditions, and provide good strain and culture technology for the development of biological phosphate fertilizer.【Method】A strain SH16(Talaromyces flavus)seperated from poplar root segments was chosen to systematically study its ability of dissolving insoluble phosphate by using Mo-Sb colorimetry and vanadium molybdate method. Using calcium phosphate as phosphorus source, factors that affect concentration of soluble phosphorus were also studied by controlling variables.【Result】 Strain SH16 could dissolve calcium phosphate, calcium superphosphate, aluminum phosphate, and phytic acid calcium. The soluble phosphorus concentration could get to a higher level when choosing calcium phosphate as phosphorus source, which could reach 660.9 mg/L after 7 days treatments, its phytase activity could reach 45 U/L. In calcium phosphate medium, carbon source, original pH value, culture temperature, and NaCl concentration could significantly affect the ability of strain SH16 to dissolve phosphate. When adding soluble phosphorus with concentration of 50 mg/L, pH=8, glucose as carbon source culture, temperature is 30 ℃, strain SH16 could dissolve more calcium phosphate. Also, strain SH16 could tolerate NaCl stress, as the content of NaCl increase, the ability of strain SH16 to dissolve phosphate decrease, when adding 5% NaCl in medium, the soluble phosphorus concentration could reach 173.1 mg/L.【Conclusion】 The strain SH16 could obviously dissolve the calcium phosphate, and it can be further developed as bio-fertilizer.