Screening for endophytic antagonistic bacterium of Jacaranda acutifolia 
and biocontrol effect against stem rot

doi:10.3969/j.issn.1000-2006.201610019

Screening for endophytic antagonistic bacterium of Jacaranda acutifolia and biocontrol effect against stem rot

ZHU Hanmingyue, PAN Yuanzhi

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (06) : 61-67.

PDF(2215534 KB)

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (06) : 61-67. DOI: 10.3969/j.issn.1000-2006.201610019

Screening for endophytic antagonistic bacterium of Jacaranda acutifolia and biocontrol effect against stem rot

ZHU Hanmingyue, PAN Yuanzhi^*

Author information +

History +

Abstract

【Objective】This study was aimed at screening antagonistic endophytic strains against stem rot in Jacaranda acutifolia, and investigating the antagonistic activity. 【Method】 Endophytic bacteria were isolated by dilution plating. F. chlamydosporum served as the test fungus, and antagonistic strains was obtained firstly by the five-point confrontation method and secondly by plate diffusion. Strains were identified by analyses of morphology and 16S rDNA sequence. Biocontrol effect was analyzed by greenhouse and field experiments. 【Results】The number of endophytic strains obtained was 24; the strain zhu66 with an inhibition rate of 97.8% was screened from the 24 strains. It was identified as Bacillus amyloliquefaciens by the 16S rDNA sequence analysis. Its GenBank accession number was JF899258. The greenhouse pot experiment showed that B. amyloliquefaciens could effectively prevent and treat stem rot in J. acutifolia. At a certain concentration, the higher the concentration, the better the anti-effect, and 10⁷ cfu/mL was at the optimal concentration.The control effect of sterilized soil was stronger than that of the natural soil. The control effect of the biocontrol strain inoculated before pathogen inoculation was stronger than that of the biocontrol strain and pathogen inoculated simultaneously; the control effect of pathogen inoculated before biocontrol strain inoculation was weak. It could promote growth of stems, and increase height and crown diameter of J. acutifolia. The higher the concentration, the stronger the effect. The field experiment further verified that the biocontrol effect on stem rot in J. acutifolia at different degrees showed remarkable differences. The preventive effect in the uneffective region was remarkably considerable,which had no stem rot. There was virtually no effect in the severely affected areas, although obvious control effect was observed in the mildly affected areas reaching 74.1%. There was a certain effect in the moderately affected areas, and with time, the control effect was enhanced.【Conclusion】B. amyloliquefaciens zhu66 strain could potentially serve as a biocontrol agent against stem rot.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHU Hanmingyue, PAN Yuanzhi. Screening for endophytic antagonistic bacterium of Jacaranda acutifolia and biocontrol effect against stem rot[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2017, 41(06): 61-67 https://doi.org/10.3969/j.issn.1000-2006.201610019

References

[1] 周静, 张炜, 李大明, 等. 蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证[J]. 林业科学研究, 2013, 26(4): 414-419. DOI:10.3969/j.issn.1001-1498.2013.04.004. ZHOU J, ZHANG W, LI D M, et al. Sequence analysis and chilling resistance about a new CBF-like trascription factor fragment from Jacaranda acutifolia[J]. Forest Reasearch, 2013, 26(4): 414-419.
[2] 朱涵明月, 李西, 朱天辉. 蓝花楹茎腐病——病原与系统发育[J]. 东北林业大学学报, 2015, 43(5): 112-117. ZHU H M Y, LI X, ZHU T H.Study on the pathogen indentification of Jacaranda acutifolia stem rot and its phylogenetic analysis[J]. Journal of Northeast Forestry University, 2015, 43(5): 112-117.
[3] EMMERT E A B, HANDELSMAN J. Biocontrol of plant disease: a(Gram-)positive perpective[J]. FEMS Microbiology Letters, 1999, 171(1): 1-9.
[4] YANG M M, WEN S S, MAVRODI D, et al. Biological control of wheat root diseases by the CLP-producing strain Pseudomonas fluorescens HC1-07[J]. Phytopathology, 2014, 104(3): 248-256. DOI:10.1094/PHYTO-05-13-0142-R· S ource:PubMed
[5] YOSHIDA S, HIRADATE S, TSUKAMOTO T, et al. Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves[J]. Phytopathology, 2001, 91(2): 181-187.
[6] KHAMNA S, YOKOTA A, LUMYONG S. Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production[J]. World Journal of Microbiology and Biotechnology, 2009, 25(4): 649-655. DOI: 10.1007/s11274-008-9933-x.
[7] 刘淑芬, 韩宝坤. 植物内生菌研究综述[J]. 邯郸农业高等专科学校学报, 2000, 17(2): 15-20. LIU S F, HAN B K. The study of endophytic fungi in plants[J]. Journal of Handan Agricultural College,2000, 17(2): 15-20.
[8] 李姝江, 朱天辉, 谯天敏, 等. 松烂皮病内生拮抗细菌的筛选、生防效果及华山松的生理响应[J]. 植物保护学报,2014, 41(5): 569-577. LI S J, ZHU T H, QIAO T M, et al.Screening of endophytic antagonistic bacterium and its biocontrol effect on dieback fungus Cenangium ferruginosum and physiological response of Chinese white pine Pinus armandii[J]. Journal of Plant Protection, 2014, 41(5): 569-577.
[9] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998.
[10] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.
[11] 刘洋, 朱天辉, 郑磊, 等. 一株内生拮抗细菌的分离鉴定及其抗菌机理研究[J]. 植物保护, 2016, 42(1): 33-39. DOI:10.3969/j.issn.0529-1542.2016.01.006. LIU Y, ZHU T H, ZHENG L,et al. Isolation, identification and antimicrobial mechanism of an endophtic antagonistic bacterium[J]. Plant Protection,2016, 42(1): 33-39.
[12] 张会敏, 冯友军. 一株野生细菌的16Sr DNA序列分析与系统发育的构建[J]. 生物信息学, 2005, 3(1): 1-4. ZHANG H M, FENG Y J. 16Sr DNA sequence analysis and phylogenic tree construction of a strain of filed bacterium[J]. China Journal of Bioinformatics, 2005, 3(1): 1-4.
[13] 孔庆科, 丁爱云. 内生细菌作为生防因子的研究进展[J]. 山东农业大学学报(自然科学版), 2001, 32(2): 256-260. KONG Q K,DING A Y, Advances of study on endophytic bacteria as biological control agents[J]. Journal of Shandong Agricultural University(Natural Science), 2001, 32(2): 256-260.
[14] FLURKEY W H. In vitro biosynthesis of Vicia faba polyphenoloxidase[J]. Plant Physiology, 1985, 79(2): 564-567.
[15] 郝建安, 曹志辉, 赵凤梅, 等. 解淀粉芽孢杆菌NK10BAhjaWT抑真菌作用的研究[J]. 微生物学通报, 2008,35(6): 903-908. DOI:10.13344/j.microbiol.china.2008.06.008. HAO J A, CAO Z H, ZHAO F M, et al. Exploring the antifungal activity of Bacillaus amyloquefaciens NK10BAhjaWT[J]. Microbiology, 2008,35(6): 903-908.
[16] 仇艳肖. 黄瓜灰霉病高效拮抗菌的筛选鉴定及其作用研究[D]. 石家庄:河北师范大学, 2010.
[17] MIKKOLA R, ANDERSSON M, GRIGORIEV P, et al. Bacillus amyloliquofaciens strains isolated from moisture-damaged buildings produced surfactin and a substance toxic to mammalian cells[J]. Archives of Microbiology, 2004,181(4): 314-323.
[18] JOSEPH O, LISE K. Integrated control of citrus green and blue molds using Bacillus subtilis in combination with sodium bicarbonate or hot water[J]. Postharvast Biology and Technology,2003,28(1):187-194. Doi:10.1016/s0925-5214(02)00145-x.
[19] 谯天敏, 张静, 冉晓潇, 等. 解淀粉芽孢杆菌在山茶叶中的定殖及对山茶灰斑病的防效[J]. 西北农林科技大学学报(自然科学版), 2015, 43(10): 77-84. DOI:10.13207/j,cnki.jnwafu.2015.10.011. QIAO T M, ZHANG J,RAN X X,et al.Colonization of Bacillus amyloliquefaciens in Camellia leaves and its control efficacy against Camellia gray spot[J]. Journal of Northwest A&F University(Natural Science Edition),2015, 43(10): 77-84.
[20] 冯永君, 宋未. 植物内生细菌[J]. 自然杂志, 2001, 23(5): 249-252. FENG Y J, SONG W. Endophetic bacteria[J]. Chinese Journal of Nature,2001, 23(5): 249-252.
[21] 张集慧, 王春兰, 郭顺星, 等. 兰科药用植物的5种内生菌产生的植物激素[J]. 中国医学科学院学报, 1999, 21(6): 460-465. ZHANG J H, WANG C L, GUO S X, et al. Studies on the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants(Orchidacea)[J]. Acta Academiae Medicinae Sinicae,1999, 21(6): 460-465.
[22] YU G Y, SINCLAIR J B, HARTMAN G L, et al. Production of iturin a by Bacillus amyloliquefaciens suppressing Rhizoctonia solani[J]. Soil Biology and Biochemistry, 2002, 34: 955-963. DOI: 10.1016/S0038-0717(02)00027-5
[23] REVA O N, DIXELIU C, MEIJER J, et al. Taxonomic characterization and plant colonizing abilities of some bacteria related to Baicllus amyloliquefaciens and Bacillus subtilis[J]. FEMS Microbiology Ecology, 2004, 48: 249-259. DOI: 10.1016/j.femsec.2004.02.003·Source: PubMed.

PDF(2215534 KB)

Accesses

Citation

Detail

Sections

Recommended

The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

〈

〉

Please choose a citation manager

Content to export