Hypoglycemic and antioxidant activity of various polysaccharides from 
Cyclocarya paliurus leaves in vitro

doi:10.3969/j.issn.1000-2006.201610064

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (04): 6-12.doi: 10.3969/j.issn.1000-2006.201610064

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Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro

XU Jingzhu¹, WU Caie^1,2*, YING Ruifeng¹, LI Tingting¹, FAN Gongjian¹,FANG Shengzuo^2,3

1.College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2.Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing 210037, China;
3.College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2017-08-18 Published:2017-08-18

Abstract

Abstract: 【Objective】In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro. The activity of refined polysaccharides and precipitation fractionations of purified C. paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.【Method】The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro. The ability of total antioxidant, scavenging DPPH and(·OH)radicals and chelating Fe²⁺were used to evaluate the antioxidant capacity.【Result】The results showed that refined polysaccharides of C. paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase, and showed a dose-effect relationship. With the ethanol concentration increasing, the fractions obtained showed a higher inhibition activity. Refined polysaccharides of C. paliurus and different precipitation fractionations all showed a good antioxidant activity. Among different precipitation fractionations, the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing. In contrast, the abilities of scavenging(·OH)radicals and chelating Fe²⁺ were decreased.【Conclusion】Different C. paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity, which can be used for further manufacture and expoliting.

CLC Number:

XU Jingzhu, WU Caie, YING Ruifeng, LI Tingting, FAN Gongjian,FANG Shengzuo. Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(04): 6-12.

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Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro

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