Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune

LUO Peng, CAO Yuting, MO Jiaxing, WENG Huaifeng, SHI Jisen, XU Jin

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (04) : 191-196.

PDF(1735320 KB)
南京林业大学学报（自然科学版）
PDF(1735320 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (04) : 191-196. DOI: 10.3969/j.issn.1000-2006.201611006

Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune

  • LUO Peng1, CAO Yuting1, MO Jiaxing1, WENG Huaifeng2, SHI Jisen1, XU Jin1*
Author information +
History +

Abstract

【Objective】Molecular fingerprinting and genetic diversity analysis are important for protecting and managing superior germplasm resources, as well as for identifying superior clones. 【Method】In the present study, 11 SSR primer pairs were used to assess the genetic diversity and DNA fingerprints of 89 C. fortunei clones. 【Result】A total of 53 putative alleles were detected from 11 pairs of primers, with a mean of five putative alleles per locus, ranging from three to six. The number of effective alleles(Ne)ranged from 1.887 0 to 4.295 6, with a mean of 3.041 1, and the Shannon's information index ranged from 0.774 9 to 1.556 3, with a mean of 1.208 1. These findings indicate that SSR markers in C. fortunei exist a relative high polymorphism. Furthermore, according to the characteristics of the SSR markers and primer sequences, the present(recorded as 1)and absent(recorded as 0)collected from amplification by SSR markers were transferred into genetic types. By combining different genetic types code, we established DNA fingerprints of 89 C. fortunei clones, and attached a 22-digit fingerprint number to each clone. The 89 clones were also separated into five clusters based on genetic distance. 【Conclusion】SSR markers are suitable for analyzing and identifying the genetic diversity of C. fortune, and C. fortune shows moderate to high genetic diversity.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
LUO Peng, CAO Yuting, MO Jiaxing, WENG Huaifeng, SHI Jisen, XU Jin. Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(04): 191-196 https://doi.org/10.3969/j.issn.1000-2006.201611006

References

[1] BOTSTEIN D, WHITE R L, SKOINICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980, 32(3): 314-331.
[2] ABE J, XU D, SUZUKI Y, et al. Soybean germplasm pools in Asia revealed by nuclear SSRs [J]. Theoretical and Applied Genetics, 2003, 106(3): 445-453. DOI:10.1007/s00122-002-1073-3.
[3] WU K S, TANKSIEY S D. Abundance, polymorphism and genetic mapping of microsatellites in rice [J].Molecular and General Genetics, 1993, 241(1): 225-235.
[4] STRUSS D, PLIFESKE J. The use of microsatellite markers for detection of genetic diversity in barley population[J]. Theoretical and Applied Genetics, 1998, 97(1): 308-315. DOI:10.1007/s001220050900.
[5] ERCISLI S, IPEK A, BARUT E. SSR marker-based DNA fingerprinting and cultivar identification of olives(Olea europaea)[J]. Biochemical Genetics, 2011, 49(9-10): 555-561.DOI:10.1007/S10528-011-9430-2.
[6] SENIOR M L, MURPHY J P, GOODMAN M M, et a1. Utility of SSRs for determining genetic similarities an relationships in maize using an agarose gel system [J]. Crop Science, 1998, 38: 1088-1089.
[7] 李金花,俞宁. 利用荧光SSR引物分析中国油橄榄的遗传多样性[J].林业科学, 2012,48(6):47-55.DOI:10.11707/j.1001-7488.20120608. LI J H,YU N. Genetic diversity of olive cultivars in China based on fluorescent SSR markers[J]. Scientia Silvae Sinicae, 2012,48(6):47-55.
[8] WU Z Y,PETER H K. Flora of China[M/OL]. Beijing: Science Press, 2003
[2016-10-15]. http://foc.eflora.cn.x.
[9] TAKAHASHI T, TANI N, TAIRA H, et al.Microsatellite markers reveal high allelic variation in natural populations of Cryptomeria japonica near refugial areas of the last glacial period[J].Journal of Plant Research,2005, 118(2):83-90.DOI:10.1007/s10265-005-0198-2.
[10] 徐进,刘子梁,欧阳磊,等. 柳杉初级种子园遗传多样性[J].东北林业大学学报, 2014,42(4):1-5. DOI:10.13759/j.cnkk.dlxb,2014.04.001. XU J,LIU Z L,OUYANG L,et al. Genetic Diversity of Cryptomeria fortunei from primary seed orchard[J]. Journal of Northeast Forestry University, 2014,42(4):1-5.
[11] MORIGUCHI Y, TSUCHIYA S,IWATA H. Factors influencing male reproductive success in a Cryptomeria japonica seed orchard revealed by microsatellite marker analysis[J].Silvae Genetica,2007,56(5):207-214.
[12] 沈敬理,白天道,陈亚斌,等. 马尾松无性系种子园亲本指纹图谱构建[J].分子植物育种,2015,13(3):646-652.DOI:10.13271/j.mpb.013.000646. SHEN J L, BAI T D, CHEN Y B, et al. The fingerprints construction of clones in Pinus massoniana seed orchard[J]. Molecular Plant Breeding, 2015, 13(3):646-652.
[13] 艾畅,徐立安,赖焕林,等.马尾松种子园的遗传多样性与父本分析[J].林业科学, 2006, 42(11): 146-150.DOI:10.3221/j.issn:1001-7488.2006.11.026. AI C, XU L A, LAI H L, et al, Genetic diversity and paternity analysis of a seed orchard in Pinus massniana[J]. Scientia Silvae Sinicae, 2006,42(11): 146-150.
[14] 李锐.柽柳SSR标记开发及群体遗传结构分析[D].南京:南京林业大学,2007. LI R.Study on develop of EST-SSR primer and genetic structure of Tamarix chinensis[D]. Nanjing: Nanjing Forestry University,2007.
[15] ECHT C S,MAY-MARQUARDT P,HSEIH M, et al.Characterization of microsatellite markers in eastern white pine [J].Genome,1996,39:1102-1108.
[16] 高源, 刘凤之, 曹玉芬,等. 苹果属种质资源亲缘关系的SSR分析[J].果树学报, 2007, 24(2): 129-134.DOI:10.3969/j.issn.1009-9980.2007.02.001. GAO Y, LIU F Z, CAO Y F, et al. Analysis of genetic relationship for Malus germplasm resources by SSR markers[J]. Journal of Fruit Science, 2007, 24(2): 129-134.
[17] XIE R J, ZHOU J, WANG G Y, et al. Cultivar identification and genetic diversity of Chinese bayberry(Myrica rubra)accessions based on fluorescent SSR markers[J]. Plant Molecular Biology Reporter, 2011, 29(3):554-562. DOI:10.1007/s11105-010-0261-6.
PDF(1735320 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉