Soil microbial characteristics of Pinus taiwanensis and broad-leaved mixed forest at different altitudes in Fengyang Mountain

MENG Miaojing,ZHANG Jinchi,GUO Xiaoping,WU Jiasen,ZHAO Youpeng,YE Lixin,LIU Shenglong

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (04) : 209-214.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2017, Vol. 41 ›› Issue (04) : 209-214. DOI: 10.3969/j.issn.1000-2006.201611044

Soil microbial characteristics of Pinus taiwanensis and broad-leaved mixed forest at different altitudes in Fengyang Mountain

  • MENG Miaojing1,ZHANG Jinchi1*,GUO Xiaoping1,WU Jiasen1,ZHAO Youpeng1,YE Lixin2,LIU Shenglong1,2
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Abstract

【Objective】To study the soil physicochemical properties and the use of carbon sources by microorganisms, we investigated the effects of altitude change on soil microbial functional diversity in the Pinus taiwanensis broad-leaved mixed forest of Fengyang Mountain.【Method】Using the main distribution range from 1 000 m to 1 800 m of Pinus taiwanensis in Fengyang Mountain as the standard, we chose three altitude gradients, respectively: medium altitude(1 200 m), middle-high altitude(1 500 m), and high altitude(1 800 m). We selected the best site in each altitudinal gradient drainage, on a sunny, gentle gradient slope, with slow, moderate growth in three standard plots(20 m×20 m). We applied the method of Biolog Eco Plate to study the carbon utilization of soil microorganisms in this type of forest at different altitude gradients. We used non-metric multidimensional scaling(NMDS)to order and further analyze the use of various carbon sources by soil microbes. 【Result】① The soil microbial activity was the highest at the middle-high altitude, whereas the soil microbial diversity index did not change significantly with altitude(P>0.05).② In the study area, the main types of carbon source used were carbohydrates(CH), amino acids(AA), and polymers(PM).The utilization rate of phenolic acids(PA)and amine(AM)carbon sources varied with altitude. There were significant differences in the utilization of the carbon source carboxylic acid(CA)at different altitudes. The NMDS ordination showed that the use of six carbon sources varied with the change in altitude(P<0.05).③The soil nutrient content was the highest in the middle-high altitude areas, and the redundancy analysis showed that there was a strong correlation between the physicochemical properties of soil and the utilization of carbon. 【Conclusion】In the middle-high altitude areas, soil microbes have a strong effect on the utilization of various carbon sources, and the change in soil physical and chemical properties is an important factor affecting the utilization of carbon sources.

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MENG Miaojing,ZHANG Jinchi,GUO Xiaoping,WU Jiasen,ZHAO Youpeng,YE Lixin,LIU Shenglong. Soil microbial characteristics of Pinus taiwanensis and broad-leaved mixed forest at different altitudes in Fengyang Mountain[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2017, 41(04): 209-214 https://doi.org/10.3969/j.issn.1000-2006.201611044

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