Intracellular proteome analysis of Piptoporus betulinus induced by different wood substrates

doi:10.3969/j.issn.1000-2006.201702019

YAN Shaopeng,ZI Xiaoxue,WANG Qiuyu

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (01) : 181-185.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (01) : 181-185. DOI: 10.3969/j.issn.1000-2006.201702019

Intracellular proteome analysis of Piptoporus betulinus induced by different wood substrates

YAN Shaopeng,ZI Xiaoxue,WANG Qiuyu

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Abstract

【Objective】In this paper, we investigated the differential expression of intracellular proteins induced by different woody substrates, and explored the degradation mechanism of wood by Piptoporus betulinus.【Method】Wood sawdust of birch and spruce were used as inducing substrates, the protein two-dimensional electrophoresis technique(2-DE)and mass spectrometry(MALDI-TOF / TOF MS)was used to detect the changes in Piptoporus betulinus intracellular protein induced by wood sawdust compared with the control group(without sawdust), and analyze the differences in bioinformatics.【Result】The Piptoporus betulinus intracellular protein expression changed significantly induced by birch and spruce sawdust. A total of 32 intracellular differential expression protein sites were detected in the treatment group(adding sawdust), 17 differential protein sites were successfully identified by mass spectrometry and database retrieval. These proteins had transferase, hydrolase, protein binding, and cell signal transduction molecular functions, and were involved in the stimulation of organic compounds, metabolism and metabolic processes.【Conclusions】The degradation of wood by Piptoporus betulinus is the result of the interaction of various proteins, regulating the expression of related proteins in various metabolic processes.

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YAN Shaopeng,ZI Xiaoxue,WANG Qiuyu. Intracellular proteome analysis of Piptoporus betulinus induced by different wood substrates[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(01): 181-185 https://doi.org/10.3969/j.issn.1000-2006.201702019

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