Characterization of gene expression in anthocyanin synthesis
 and salt tolerance of Betula pendula ‘Purple Rain'

doi:10.3969/j.issn.1000-2006.201704048

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (02): 25-32.doi: 10.3969/j.issn.1000-2006.201704048

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Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'

LYU Donglin,LIN Lin,GUO Yiwen, HAN Rui,JIANG Jing^*

State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040, China

Online:2018-04-12 Published:2018-04-12

Abstract

Abstract: 【Objective】 To understand the mechanism of salt tolerance, we examined a possible relationship between salt tolerance and gene expression for anthocyanin biosynthesis in salt tolerant Betula pendula ‘Purple Rain'.【Methods】 We used Betula pendula ‘Purple Rain' and Betula pendula ‘Roth'(control)to measure chlorophyll fluorescence and other physiological indicators under salt stress. We analyzed gene expression of chalcone synthase(CHS), dihydlroflavonol-4-reductase(DRF), and MYB transcription factor related to anthocyanin biosynthesis using RT-PCR. 【Result】 The results showed that anthocyanin was 3.4 times higher in ‘Purple Rain,' whereas salt damage indexes were significantly lower than those of ‘Roth' after 12 d under 0.8% NaCl stress. The reswlts of DAB and NBT staining showed that accumulation of O^-₂ and H₂O₂ in leaves of ‘Purple Rain' was lower than that of ‘Roth'. The F_v/F_m value for ‘Purple Rain' was within the normal range after 12 d under NaCl stress, and the qP and Φ_PS_Ⅱ values were significantly lower than those of ‘Roth'(P < 0.05). The NPQ was 282.29% and was significantly higher than that of ‘Roth'(P < 0.01). Gene expression of BpCHS2,BpDFR5, and BpMYB10, related to anthocyanin biosynthesis, were continuously up-regulated under NaCl stress. 【Conclusion】 Anthocyanin-rich ‘Purple Rain' can tolerate a certain range of NaCl stress. The BpMYB10, BpCHS2, and BpDFR5 of ‘Purple Rain' exhibited a different expression pattern in comparison with ‘Roth', and this might be closely related to anthocyanin biosynthesis. Anthocyanin can enhance the ability of ‘Purple Rain' to remove reactive oxygen radicals, slow down oxidation of membranes, and improve salt tolerance.

CLC Number:

S722.5

LYU Donglin,LIN Lin,GUO Yiwen, HAN Rui,JIANG Jing. Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(02): 25-32.

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Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'

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