Effects of inoculation ways on nodule formations of precious timber tree 
species Ormosia henryi seedlings

doi:10.3969/j.issn.1000-2006.201705049

Effects of inoculation ways on nodule formations of precious timber tree species Ormosia henryi seedlings

AN Changrong, WEI Xiaoli, DUAN Ruyan, YE Run, LI Xingwei

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (02) : 9-16.

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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (02) : 9-16. DOI: 10.3969/j.issn.1000-2006.201705049

Effects of inoculation ways on nodule formations of precious timber tree species Ormosia henryi seedlings

AN Changrong¹, WEI Xiaoli^1*, DUAN Ruyan^1,2, YE Run¹, LI Xingwei¹

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Abstract

【Objective】The aim of this study was to explore the effects of rhizobium inoculation methods on Ormosia henryi seedlings.【Method】We designed a potting test that included four inoculation methods: soaking the seed, soaking the radicle, mixing rhizobium liquid with the seed, and pouring rhizobium liquid, which non-inoculated rhizobium as a control. We observed the root morphology and quantity of nodules, and determined some indexes that relate to growth, physiology, and biochemistry as well as nitrogen fixation. 【Result】The result showed that the roots of the seedlings were loose, soft and appear khaki, most of the young nodules are spherical and appear yellowish-brow, and most nodules grew from the lateral root. The quantity and quality of nodules were significantly different among the different inoculation methods; the seedlings inoculated by pouring rhizobium showed a higher number and weight of nodules and a higher nodulation rate than those by other methods, and the seedlings inoculated by soaking the radicle were second highest. The growth indices of Ormosia henryi seedlings with nodules were bigger than that of the control. Compared to the control, the height, ground diameter, and biomass of seedlings treated by four inoculation methods increased by 11.48%-29.51%, 3.21%-26.61% and 36.30%-148.18%, respectively; the total root length, total root surface area, average root diameter, total root volume, and root tip number increased by 70.62%-139.39%, 74.70%-140.97%, 164.28%-200.00%, 75.00%-250.00% and 28.03%-167.42%, respectively. The physiological and biochemical indices of seedlings with nodules by different inoculation methods showed significant difference. Under the pouring rhizobium method, the chlorophyll content, nitrate nitrogen content, nitrate reductase activity, and root activity were significantly higher than those seen in other treatments. The total nitrogen content, hemoglobin content, total nitrogen content, and nitrogen fixation rate of inoculated seedling leaves were significantly higher than those without inoculated rhizobium, and the nitrogen fixation effect in the seedlings inoculated by pouring rhizobium liquid and soaking the radicle were higher than those in the other treatment groups.【Conclusion】The most suitable methods of inoculation are soaking the roots of germination seeds and irrigating the Ormosia henryi seedlings using rhizobium liquid when nodules grow on the seedlings.

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AN Changrong, WEI Xiaoli, DUAN Ruyan, YE Run, LI Xingwei. Effects of inoculation ways on nodule formations of precious timber tree species Ormosia henryi seedlings[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2018, 42(02): 9-16 https://doi.org/10.3969/j.issn.1000-2006.201705049

References

[1] 陈文新,汪恩涛.中国根瘤菌[M].北京:科学出版社,2011:3-4.
[2] 窦雅静,康丽华,陆俊锟.相思属树种根瘤菌研究进展[J].安徽农业科学,2013,41(17):7511-7514.DOI:10.3969/j.issn.0517-6611.2013.17.042. DOU Y J,KANG L H,LU J K.Research progress of rhziobia associated with Acacia tress[J].Journal of Anhui Agriculture Science,2013,41(17):7511-7514.
[3] 邓兆,韦小丽,孟宪帅,等.花榈木种子休眠和萌发的初步研究[J].贵州农业科学,2011,39(5):69-72.DOI:10.3969/j.issn.1001-3601.2011.05.020. DENG Z,WEI X L,MENG X S,et al.A preliminary study on dormancy and germination of Ormosia henryi seeds[J].Guizhou Agricultural Sciences,2011,39(5):69-72.
[4] 韦小丽,孟宪帅,邓兆.珍稀树种花榈木种子繁殖生态学特性与濒危的关系[J].种子,2014,33(1):82-86.DOI:10.3969/j.issn.1001-4705.2014.01.024. WEI X L,MENG X S,DENG Z.Relation between being endangered and seed reproductive ecology of a rare species Ormosia henryi[J]. Seed,2014,33(1):82-86.
[5] 邓兆,韦小丽.珍贵树种花榈木种子休眠破除方法研究[J].种子,2016,35(11):1-4.DOI:10.16590/j.cnki.1001-4705.2016.11.001. DENG Z,WEI X L.Study on method of breaking seed dormancy for rare tree species Ormosia henryi[J]. Seed,2016,35(11):1-4.
[6] 高丽,李红林,杨波.花榈木胚轴愈伤组织的诱导及植株再生[J].安徽农业科学,2009,37(3):16271-16273.DOI:10.13989/j.cnki.0517-6611.2009.33.129. GAO L,LI H L,YANG B.Callus induction and plant regeneration from the hypocotyl of Ormosia henryi prain[J].Journal of Anhui Agricultural Sciences,2009,37(3):16271-16273.
[7] 姚军,李红林,杨波.花榈木的组织培养和快速繁殖[J].植物生理学通讯,2007,43(1):123-124.DOI:10.13592/j.cnki.ppj.2007.01.035. YAO J,LI H L,YANG B.Tissue culture and rapid propagation of Ormosia henryi prain[J]. Plant Physiology Communications,2007,43(1):123-124.
[8] 乔栋,韦小丽,李群.珍贵树种花榈木组培不同外植体的无菌繁殖体系构建[J].西南农业学报,2016,29(7):1719-1723.DOI: 10,16213 /j.cnki.scjas. 2016.07.039. QIAO D,WEI X L,LI Q.Establishment of sterile propagation system of different explant for rare species Ormosia henryi in tissue culture[J].Southwest China Journal of Agricultural sciences,2016,29(7):1719-1723.
[9] 段如雁,韦小丽,张怡,等.花榈木容器育苗的基质筛选[J].林业科技开发,2015,29(4):27-31.DOI:10.13360/j.issn.1000-8101.2015.04.006. DUAN R Y,WEI X L,ZHANG Y,et al.Screening on substrate of container seedlings for rare species[J].China Forestry Science and Technology,2015,29(4):27-31.
[10] 姜顺邦,韦小丽.供水量对花榈木苗期耗水、生长和生理的影响及灌溉制度优化[J].林业科学,2016,52(10):22-30.DOI:10.11707/j.1001-7488.20161003. JIANG S B,WEI X L.Effcets of water supply on water consumption,growth and physiological processes of Ormosia henryi seedlings and the irrigation system optimization[J].Science Silvae Sinicae,2016,52(10):22-30.
[11] 段如雁,韦小丽,孟宪帅.不同光照条件下花榈木幼苗的生理生化响应及生长效应[J].中南林业科技大学学报,2013,33(5):30-34. DUAN R Y,WEI X L,MENG X S. Physiological and biochemical responses and growth effects of Ormosia henryi seedlings under different light conditions[J].Journal of Central South University of Forestry & Technology,2013,33(5):30-34.
[12] 孟宪帅,韦小丽.不同水分环境对花榈木幼苗生理生化的影响[J].山地农业生物学报,2011,30(3):215-220.DOI:10.3969/j.issn.1008-047.2011.03.006. MENG X S, WEI X L.Effects of water conditions on the physiological and biochemical characters of young Ormosia henryi seedlings[J].Journal of Mountain Agriculture and Biology,2011,30(3):215-220.
[13] 韩素芬,林树燕.豆目树种与根瘤菌[M].北京:中国林业出版社,2013:15-37.
[14] 李东,黄莉莉,韩素芬.23株豆科木本植物根瘤菌16SrDNA序列分析[J].南京林业大学学报(自然科学版),2007,31(6):117-121.DOI:10.3969/j.issn.1000-2006.2007.06.028. LI D,HUANG L L,HAN S F.16SrDNA sequence analysis of 23 rhizobium strains isolated from leguminosae woody plants[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2007,31(6):117-121.
[15] 段如雁,韦小丽,简才源,等.根瘤菌对花榈木2年生苗木生长及生理生化特性的影响[J].西部林业科学,2014,43(2):102-107.DOI:10.3969/j.issn.1672-8246.2014.02.017 DUAN R Y,WEI X L,JIAN C Y,et al.Effect of rhizobia on growth and physiological-biochemical character of biennial Ormosia henryi seedlings[J].Journal of West China Forestry Science,204,43(2):102-107.
[16] 侯远瑞,邓艳.根瘤菌拌种培育马占相思苗木试验[J].广西林业科学,2002,31(4):204-205.DOI:10.3969/j.issn.1006-1126.2002.04.012. HOU Y R,DENG Y.Experiment on seedling cultivation of Acacia mangoensis with rhizobium[J].Guangxi Forestry Science,2002,31(4):204-205.
[17] 侯远瑞,邓艳,林莹,等.根瘤菌浸根处理对相思苗木接种的效应初报[J].广西林业科学,2004,33(2):88-89.DOI:10.3969/j.issn.1006-1126.2004.02.012. HOU Y R,DENG Y,LIN Y,et al.Effects of rhizosphere leaching on seedling of Acacia[J].Guangxi Forestry Science,2004,33(2):88-89.
[18] 张洪娟.豆科树种根部解剖结构和化学物质与结瘤的关系[D].南京:南京林业大学,2004:19-20. ZHANG H J. The relationship between the root anatomical structure and chemical substance and nodulation in legume[D].Nanjing: Nanjing Forestry University,2004: 19-20.
[19] 郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2007:87-135.
[20] 张志良,瞿伟菁,李小方.植物生理学实验指导[M].3版.北京:高等教育出版社,2007:69-105.
[21] 左永梅,刘永秀,张福锁.与玉米混作改善花生铁营养对其根瘤形态结构及豆血红蛋白含量的影响[J].植物生理与分子生物学报,2003,29(1):33-38.DOI:10.3321/j.issn:1671-3877.2003.01.006. ZUO Y M,LIU Y X,ZHANG F S.Effects of improvement of iron nutrition by mixed cropping with maize on nodule microstructure and leghaemoglobin content of peanut[J].Journal of Plant Physiology and Molecular Biology,2003,29(1):33-38.
[22] MARTENSSON A M, LJUNGGREM H D. A comparsion between the acetylene reduction method, the isotope dilution method and the total nitrogen difference method for measuring nitrogen fixation in lucern(Medicago sativa L.)[J]. Plant and Soil,1984,81:177-184. DOI:10.1007/BF02197149.
[23] 赵亚兰,尉亚辉.豆血红蛋白的研究进展[J].西北植物学报,2000,20(4):684-689.DOI:10.3321/j.issn:1000-4025.2000.04.031 ZHAO Y L,WEI Y H.Progress in the study of leg-hemoglobin[J].Acta Botanica Boreali Occidentalla Sinica,2000,20(4):684-689.
[24] 康丽华,李素翠.相思苗木接种根瘤菌的研究[J].林业科学研究,1998,11(4):343-348. KANG L H,LI S C.Response of Acacias to rhizobia inoculation[J].Forest Research,1998,11(4):343-348.
[25] 周湘泉,韩素芬.豆科树种根瘤菌共生体系的研究[J].南京林业大学学报(自然科学版),1984,8(2),32-41. DOI: 10.3969/j.issn.1000-2006.1984.02. ZHOU X Q,HAN S F.Studies on symbiotic system of nodule bacteria and tree legumes[J].Journal of Nanjing Forestry University(Natural Sciences Edition),1984,8(2):32-41.
[26] 张洪娟,韩素芬,韩正敏.豆科木本植物根部特征与结瘤的关系[J].林业科技开发,2006,20(4):20-23.DOI:10.3969/j.issn.1000-8101.2006.04.006 ZHANG H J,HAN S F,HAN Z M.The relationship between the root structure of wood legume plants and their nodulation[J].China Forestry Science and Technology,2006,20(4):20-23.
[27] 韩素芬,李东.刺槐根瘤发育过程外部形态和结构的观察[J].南京林业大学学报(自然科学版),1993,17(2):35-38. DOI: 10.3969/j.issn.1000-2006.1993.02. HAN S F,LI D.Studies on the shape and structure of locust nodules in the process of its development[J].Journal of Nanjing Forestry University(Natural Sciences Edition),1993,17(2):35-38.
[28] HEICHEI G H, VANCE C P. Nitrate-N and rhizobium strain roles in alfalfa seedling nodulation and growth[J].Crop Science,1979,19(7):512-519.
[29] 吕成群,黄宝灵,韦原莲,等.不同相思根瘤菌株接种厚荚相思幼苗效应的比较[J].南京林业大学学报(自然科学报),2003,27(4):15-18.DOI:10.3969/j.issn.1000-2006.2003.04.004. LV C Q,HUANG B L,WEI Y L,et al.Comparative effects of different Acacia rhizobia inoculated on Acacia crassicarpa seedlings[J].Journal of Nanjing Forestry University(Natural Sciences Edition),2003,27(4):15-18.
[30] 张慧,余永昌,黄宝灵,等.接种根瘤菌对直杆型大叶相思幼苗生长及土壤营养元素含量的影响[J].东北林业大学学报,2005,33(5)47-50.DOI:10.3969/j.issn.1000-5382.2005.05.017. ZHANG H,YU Y C,HUANG B L,et al.Effects of rhizobia inoculation on the growth of straight-stem Acacia auriculaefom is seedlings and the contents of soil nutritive elements[J].Journal of Northeast Forestry University,2005,33(5):47-50.

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