Study on tissue culture and rapid propagation of Cerasus cerasoides var. cerasoides

ZHANG Fan, YE Lu, DONG Jifei, WAN Xueqin, ZHONG Yu

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (03) : 111-116.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (03) : 111-116. DOI: 10.3969/j.issn.1000-2006.201706047

Study on tissue culture and rapid propagation of Cerasus cerasoides var. cerasoides

  • ZHANG Fan1, YE Lu1, DONG Jifei1, WAN Xueqin2*, ZHONG Yu2
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Abstract

Abstract: 【Object】 The aim of this study was to resolve the problems of low induction rates, low proliferation rate, and the difficulty of plantlet rooting in Cerasus cerasoides var. cerasoides, and to establish a rapid propagation system through selecting the suitable method for disinfection of C. cerasoides var. cerasoides and optimizing culture conditions. 【Methods】 The effects of sterilization methods, types of basic medias(MS, 1/2 MS and 1/4 MS)and the plant hormones(6-BA, NAA and IBA)on single bud, cluster buds and rooting were investigated using stems with axillary buds of Cerasus cerasoides var. cerasoides as explants. 【Results】 When the stem with axillary buds was sterilized with 75% alcohol for 20 s and 0.1% HgCl2 for 300 s, the contamination rate was the lowest. MS medium supplemented with 1.2 mg/L 6-BA and 0.1 mg/L IBA showed the highest clustered bud induction rate(82.22%). MS medium with 0.8 mg/L 6-BA, 0.06 mg/L IBA and 0.08 mg/L NAA exhibited the highest clustered bud proliferation rate(7.32). Half MS medium with 0.5 mg/L IBA was suitable for plantlet rooting, and the rooting rate was 92.22%.【Conclusions】The optimal sterilization method for stem segments of C. cerasoides var. cerasoides with axillary buds was the use of 75% alcohol for 20 s and 0.1% HgCl2 for 300 s. The optimal medium for clustered bud induction was MS containing 1.2 mg/L 6-BA and 0.1 mg/L IBA. The optimal medium for proliferation of the clustered buds was MS supplemented with 0.8 mg/L 6-BA, 0.06 mg/L IBA, and 0.08 mg/L NAA, while the optimal rooting medium was 1/2 MS containing 0.5 mg/L IBA. The survive rate was above 72.2% when the plantlets were due to be moved to the nursery. The medium with 25% sand, 25% humus and 50% roseite was effective for rooting. The results of this study can provide a basis for large-scale propagation of C. cerasoides var. cerasoides.

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ZHANG Fan, YE Lu, DONG Jifei, WAN Xueqin, ZHONG Yu. Study on tissue culture and rapid propagation of Cerasus cerasoides var. cerasoides[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2018, 42(03): 111-116 https://doi.org/10.3969/j.issn.1000-2006.201706047

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