Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.

doi:10.3969/j.issn.1000-2006.201709001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (04): 113-118.doi: 10.3969/j.issn.1000-2006.201709001

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Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.

DONG Jingxiang, REN Li, ZHANG Yuan, YANG Yang, HUANG Haijiao, LI Huiyu^*

School of Forestry, Northeast Forestry University,Harbin 150040, China

Online:2018-07-27 Published:2018-07-27

Abstract

Abstract: Abstract: 【Objective】To analyze the sequence features and gene expression patterns of TCP transcription factors from Betula platyphylla Suk. in different tissues and time points. 【Method】Based on 45 birch transcriptome databases, 15 BpTCP genes were identified and the sequence features and gene expression at different time points and in different tissues(leaves, terminal buds, stems, male inflorescences and female inflorescences)were analyzed.【Result】Bioinformatic analysis showed that all 15 BpTCPs contained a highly conserved bHLH domain. Phylogenetic analysis showed that the 15 birch TCP proteins belonged to two major categories.The qRT-PCR results suggested that the expression levels of the 15 BpTCP genes changed significantly in terminal buds and that most BpTCP genes were up-regulated from May to September. In stems, BpTCP3 exhibited an increasing pattern during the full growth period; BpTCP4, BpTCP10, BpTCP7 and BpTCP8 were up-regulated in male and female inflorescences after winter.【Conclusion】This study may provide information for further research on the functional characteristics of BpTCPs gene and the molecular mechanism(s)involved in the regulation of plant growth.

CLC Number:

S792
Q781

DONG Jingxiang, REN Li, ZHANG Yuan, YANG Yang, HUANG Haijiao, LI Huiyu. Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(04): 113-118.

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Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.

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