Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

doi:10.3969/j.issn.1000-2006.201709011

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2017, Vol. 41 ›› Issue (06): 41-46.doi: 10.3969/j.issn.1000-2006.201709011

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Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

CHENG Tielong^1,2, MENG Yan^1,3,CHEN Jinhui^1,3,SHI Jisen^1,3*

1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 3. Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2017-12-18 Published:2017-12-18

Abstract

Abstract: 【Object】 In order to further increase the frequency of somatic embryogenesis, the experiment was conducted to investigate the effect of methyl jasmonate(MeJA)on somatic embryogenesis development of Liriodendron hybrid. 【Methods】Based on an established system of somatic embryogenesis of Liriodendron hybrid, embryogenic cells of three genotypes included C138, 233012 and 253010 from Liriodendron chinense×L.tulipifera were cultured in the embryo induction medium with different concentrations of MeJA, and the rates of somatic embryogenesis, somatic embryo maturation, abnormal somatic embryos, and number of regeneration plants were investigated. 【Result】 The different concentrations of MeJA can significantly affect the efficiency of Liriodendron hybrid somatic embryogenesis, and with the increase of MeJA concentration, the rates of somatic embryogenesis and maturation gradually increased, the rate of abnormal embryos decreased gradually. However, when the MeJA concentration exceeds a certain value, the rates of somatic embryogenesis and maturation showed a decreased trend, and the number of abnormal embryos also increased. Of which genotype 233012 was in the first place as well as genotype C138 was higher than 253010. Abscisic acid(ABA)could enhance the above-mentioned effects of MeJA. But the increasing concentration of MeJA restricted the root growth, and ABA could not alter this effect. 【Conclusion】The induction effect of Liriodendron hybrid somatic embryos could be optimal when adding 1 mol/L MeJA and 2 mg/L ABA, the rates of somatic embryogenesis and somatic embryo maturation were relatively high, with the lowest rate of abnormal embryos.

CLC Number:

S722.8

CHENG Tielong, MENG Yan,CHEN Jinhui,SHI Jisen. Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(06): 41-46.

References

[1] ELHITI M, STASOLLA C. The use of zygotic embryos as explants for in vitro propagation: an overview [J]. Methods Mol Biol, 2011, 710: 229-255. DOI:10.1007/978-1-988-8_17.
[2] 季孔庶,王潘潘,王金铃,等. 松科树种的离体培养研究进展[J]. 南京林业大学学报(自然科学版), 2015, 39(1): 142-148. DOI: 10.3969/j.issn.1000-2006.2015.01.001. HUO J Z, WANG P P, WANG J L, et al.Review on in vitro culture of tree species in Pinaceae[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015, 39(1): 142-148. DOI: 10.3969/j.issn.1000-2006.2015.01.001.
[3] MONTEVVIS O. Micorpropagation and production of forest trees [C]//Suwon: National Institute of Forest Science, 2017: 32-55.
[4] 王章荣.鹅掌楸属树种杂交育种与利用[M]. 北京: 中国林业出版社, 2005:16-34.
[5] 陈金慧.杂交鹅掌揪体细胞胚胎发生研究[D]. 南京:南京林业大学, 2003.
[6] 冯大领,孟祥书,王艳辉,等. 植物生长调节剂在植物体细胞胚发生中的应用[J]. 核农学报,2007,21(3): 256-260. DOI:10.3969/j.issn.1000-8551.2007.03.013. FENG D L, MENG X S, WANG Y H, et al. Application of exogenous plant growth regulator in plant somatic embryogenesis[J]. Journal of Nuclear Agricultural Sciences, 2007, 21(3): 256-260.
[7] JIMÉNEZ V M, BANGERTH F. Endogenous hormone concentrations and embryogenic callus development in wheat[J].Plant Cell,Tissue and Organ Culture,2001,67(1):37-46. DOI:10.1007/978-1-988-8_17.
[8] 马国华, 许秋生, 羡蕴兰. 几种生长素对木薯体细胞胚发生和植株再生的作用[J]. 热带亚热带植物学报, 1999, 7(1): 75-80.
[9] 齐力旺,韩一凡,韩素英,等. 麦芽糖、NAA及ABA对华北落叶松体细胞胚成熟及生根的影响[J].林业科学,2004,40(1): 52-57. DOI:10.3321/j.issn:1001-7488.2004.01.009. QI L W, HAN Y F, HAN S Y, et al. Effects of maltose, NAA and ABA on somatic maturation and radicle rooting of Larix principis-rupprechtii[J]. Scientia Silvae Sinicae, 2004, 40(1): 52-57.
[10] 李卫, 张春广, 郭光沁,等. ABA诱导毛酸浆下胚轴体细胞胚状结构的直接发生[J]. 西北植物学报, 2003, 23(2): 309-313. DOI:10.3321/j.issn:1000-4025.2003.02.020. LI W, ZHANG C G, GUO G Q, et al. Aba stimulated direct formation of embryo-like structures at globular stage from hypocotyls of physalis pubescens[J]. Acta Botanica Boreali-Occidentalia Sinica, 2003, 23(2): 309-313.
[11] 魏丕伟,施季森,赵振洲. 中国七叶树悬浮培养体细胞球形胚的增殖与发育研究[J]. 南京林业大学学报(自然科学版),2009,33(4): 13-18. DOI:10.3969/j.issn.1000-2006.2009.04.003. WEI P W, SHI J S, ZHAO Z Z. Proliferation and development of globular somatic embryos of Aesculus chinensis Bunge in suspension cultures[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2009, 33(4): 13-18.
[12] BERGER S,BELL E,MULLET J E. Two methyl jasmonate-insensitive mutants show altered expression of AtVsp in response to methyl jasmonate and wounding[J]. Plant Physiology,1996,111(2): 525-531. DOI:10.1104/pp.111.2.525.
[13] SEMBDNER G. The biochemistry and the physiological and molecular actions of jasmonates [J]. Annual Review of Plant Physiology and Plant Molecular Biology,1993,44(1): 569-589. DOI:10.1146/annurev.arplant.44.1.569.
[14] CREELMAN R A,MULLET J E. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress[J]. Proc Natl Acad Sci USA,1995,92(10): 4114-4119. DOI:10.1073/pnas.92.10.4114.
[15] 孙际薇. 茉莉酸甲酯对曼陀罗毛状根的生长及次生代谢产物产生的影响[D]. 重庆:西南大学,2014.
[16] 刘彧,赵秀兰,李欢庆. 茉莉酸甲酯抑制小麦根生长及体内抗氧化酶活性变化[J]. 安徽农业科学,2007,35(4): 988-989,1008. DOI:10.3969/j.issn.0517-6611.2007.04.026. LIU Y, ZHAO X L, LI H Q. Inhibition of methyl jasmonate on root growth and antioxidative enzyme activities in wheat[J]. Journal of Anhui Agricultural Sciences, 2007, 35(4): 988-989,1008.
[17] 淡墨.应用代谢组学策略研究茉莉酸甲酯对三七不定根次生代谢产物的影响[D].上海:上海交通大学,2008.
[18] 王瑜.不同因子对王不留行毛状根的生长及次生代谢产物的影响[D].长春:吉林农业大学,2014.
[19] WEIDHASE R A,KRAMELL H M,LEHMANN J,et al. Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments[J]. Plant Science,1987,51(2): 177-186. DOI:10.1016/0168-9452(87)90191-9.
[20] GEERTS A,FELTKAMP D,ROSAHL S. Expression of lipoxygenase in wounded tubers of Solanum tuberosum L[J]. Plant Physiol,1994,105(1): 269-277. DOI:10.1104/pp.105.1.269.
[21] 陈金慧,施季森,诸葛强,等.杂交鹅掌楸体细胞胚胎发生研究[J].林业科学,2003,39(4): 49-53. CHEN J H, SHI J S, ZHUGE Q, et al. Somatic embrygenesis of Liriodendron hybrids(L.chinese×tulipifera)[J]. Scientia Silvae Sinicae, 2003,39(4):49-53.
[22] THALER J S,STOUT M J,KARBAN R,et al. Exogenous jasmonates simulate insect wounding in tomato plants(Lycopersicon esculentum)in the laboratory and field[J]. J Chem Ecol,1996,22(10): 1767-1781. DOI:10.1007/BF02028503.
[23] 陈志,陈金慧,边黎明,等. 杂交鹅掌楸胚性细胞悬浮系的建立[J]. 分子植物育种,2007,5(1): 137-140. DOI:10.3969/j.issn.1672-416X.2007.01.025. CHEN Z, CHEN J H, BIAN L M, et al. Establishment of embryogenic callus suspension culture system in Liriodendron hybrids[J]. Molecular Plant Breeding, 2007, 5(1): 137-140.
[24] MUNDY J,YAMAGUCHI-SHINOZAKI K,CHUA NH. Nuclear proteins bind conserved elements in the abscisic acid-responsive promoter of a rice rab gene[J]. Proc Natl Acad Sci USA,1990,87(4):1406-1410. DOI:10.1073/pnas.87.4.1406.
[25] 崔凯荣,裴新梧,王亚馥,等. ABA对枸杞体细胞胚发生的调节作用[J].实验生物学报,1998,31(2):195-201. CUI K R, PEI X W, WANG Y F, et al. Effects of modutation of abscisic acid during somatic embryogenesis in Lycium barbarum L[J]. Acta Biologiae Experimentalis Sinica, 1998, 31(2): 195-201.
[26] 黄胜琴,宾金华,李政平. 茉莉酸甲酯和脱落酸对花生幼苗根和下胚轴生长的影响[J]. 植物生理与分子生物学学报,2002,28(5): 351-356. DOI:10.3321/j.issn:1671-3877.2002.05.005. HUANG S Q, BIN J H, LI Z P. Effects of methyl jasmonate and aba on the growth of root and hypocotyls of peanut seedling[J]. Journal of Plant Physiology and Molecular Biology, 2002, 28(5): 351-356.

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Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

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