Abstract: 【Objective】The giant panda(Ailuropoda melanoleuca)is a rare specie and is known as the national treasure of China. The ex-situ conservation of the giant panda is often restricted by bamboo resources. Therefore, it is important to study the nutritional composition of the bamboos, which are distributed outside the area of the giant panda. This study can provide scientific basis for the ex-situ conservation and introduction of bamboo species in reserves.【Method】The crude protein, ether extract, crude fiber, ash, nitrogen free extract, total energy, calcium, total phosphorus, energy, and protein ratio of ten kinds of bamboo(Fargesia yunnanensis, F. yuanjiangensis, F. fractiflexa, F. fungosa, Bambusa ventricosa, B. textilis, B. multiplex, B. intermedia, Neosinocalamus affinis, Dendrocalamus farinosus)leaves were analyzed in this study. The seasonal variation of nutrients in bamboo leaves were discussed in this paper.【Result】The results showed that the highest annual mean crude protein content of bamboo leaves was found in Dendrocalamus farinosus(19.54±2.76)%, whereas the lowest was in Fargesia yuanjiangensis(11.64±0.70)%. The seasonal variation of crude protein in ten kinds of bamboo leaves was obvious throughout the year. The average ether extract of Bambusa multiplex was the highest, whereas that of B. textilis was the lowest. The crude fiber content of ten kinds of bamboo leaves was not obviously different throughout the year. The highest crude fiber content was found in F. fungosa, whereas the lowest was in Neosinocalamus affinis. The seasonal changes in gross energy were not obvious in the ten kinds of bamboo leaves. The energy protein ratios of F. yuanjiangensis, F. fungosa, B. multiplex, and F. yunnanensis were significantly higher than that of N. affinis, D. farinosus, B. textilis, and B. ventricosa(P<0.05). The highest energy protein ratio of bamboo leaves was found in F. yuanjiangensis((162.44±10.17)kJ/g). The calcium, total phosphorus, and crude ash of these bamboo leaves were(0.85±0.05)%-(0.93±0.07)%,(0.15±0.01)%-(0.27±0.03)%, and(12.06±4.32)%-(16.16±3.80)%, respectively, throughout the year. The seasonal variation of calcium in ten kinds of bamboo leaves was not obvious. However, the seasonal variation of total phosphorus and ash were obvious throughout the year.【Conclusion】Our studies indicated that the nutritional composition of N. affinis, F. fractiflexa, F. yunnanensis, B. multiplex, and F. yuanjiangensis conforms to the requirements of the giant panda's staple bamboo. Whether they can be the staple bamboo of the giant panda, still needs further verification in practical feeding trials. Our results provide a scientific basis and theoretical reference for the conservation of giant pandas and the feeding of giant pandas in zoos.