Abstract: 【Objective】The flower color is the important ornamental character of Cymbidium ensifolium. In this study, components of C. ensifolium flowers were detected and provided foundation for color composition analysis and new variety cultivation. 【Method】 Firstly, the yellow-green and red petal tissues of six plants were used in this study. Secondly, the phenotypes and digital color matching were detected, and the protein content of petals and pH value were also measured. Finally, the pigment and structural composition in two flower tissues were detected by UV-Vis spectrophotometer and high performance liquid chromatography-mass spectrometry(LC-MS). 【Result】 The yellow-green petals in brightness value(L*, 70.1±1.7)was almost twice than that in red petals. The red petals in red value(a*, 51.2±1.9)was four times more than that of yellow-green petals. The yellow-green petals in yellow value(b*, 35.2±1.5)was about three times more than that of red petals. The result showed conspicuous chromaticity difference between two color petals; The red petals in protein content(3.44±0.11)mg/g was about 2 times more than that in yellow-green petals; twelve flavonol glycosides and 8 anthocyanin were inspected in these sample petals. 【Conclution】Isorhamnetin glycosides is located only in yellow-green petals, and some of which indwell only in the red petals, such as geranium-diglucoside, delphinium glucoside, kaempferol rhamnoside, cornflower rutin glycoside, kaempferol rutinoside and kaempferol rutinoside galactoside.