Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro

FAN Ziluan, ZHAO Ziroul, ZHAO Xiang, CHEN Kaili, FU Yujie

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (04) : 132-138.

PDF(1542724 KB)
南京林业大学学报（自然科学版）
PDF(1542724 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (04) : 132-138. DOI: 10.3969/j.issn.1000-2006.201809010

Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro

  • FAN Ziluan1,2, ZHAO Ziroul, ZHAO Xiang1, CHEN Kaili1, FU Yujie3*
Author information +
History +

Abstract

【Objective】 In order to further develop and utilize lingonberry(Vaccinium vitis-idaea)fruits, the total triterpenoids of lingonberry were obtained, and the purification process was optimized through using macroporous adsorption resin and secondary purification of with Sephadex LH-20. The in vitro anti-inflammatory activity of this triterpene compound was analyzed. 【Method】 The experiment was based on the wild buckwheat-hung lingonberry as the raw material. The a static adsorption-analysis experiment, and the dynamic adsorption -analysis experiment were used to select the macroporous adsorption resin, optimization of the best process, and determination of the best sample concentration, pH 6, sample volume, sample flow rate, and ethanol concentration of the eluent. The secondary purification used Sephadex LH-20 hydroxypropyl dextran gel to obtain high-purity lingonberry total triterpene. Using hyaluronidase and the inhibition of serum albumin denaturation as an anti-inflammatory evaluation index to evaluate the anti-inflammatory activity of the total triterpenes of from lingonberry. 【Result】 The purification effect of seven macroporous resins on the total triterpenes of lingonberry was compared. The results showed that the X-5 resin was most suitable for the primary purification. The optimum purification process was the sample concentration of 1.5 mg/mL, elution at pH 6, loading volume 4/3 BV, loading flow rate of 1 BV/h, and 80% ethanol as eluant. The purity of the total triterpenes of lingonberry increased from 5.13% to 29.46%. Further purification using Sephadex LH-20 obtained a purity of(43.25±0.31)%. The total anti-inflammatory activity of the lingonberry was(81.5±1.37)% for the hyaluronidase and(72.59±1.84)% for the denaturation of bovine serum albumin. 【Conclusion】 Lingonberry is a nutrient-rich berry. High-purity triterpenoids were obtained through the secondary purification technology, and it was preliminarily showed that the total triterpenes of lingonberry had significant anti-inflammatory activity. Our results provided a certain theoretical basis for the further development and utilization of lingonberry.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
FAN Ziluan, ZHAO Ziroul, ZHAO Xiang, CHEN Kaili, FU Yujie. Purification of total triterpenoids from lingonberry and anti-inflammatory activity in vitro[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(04): 132-138 https://doi.org/10.3969/j.issn.1000-2006.201809010

References

[1] BUJOR O C, GINIES C, POPA V I, et al. Phenolic compounds and antioxidant activity of lingonberry(Vaccinium vitis-idaea L.)leaf, stem and fruit at different harvest periods[J]. Food Chemistry, 2018, 252:356-365. DOI:10.1016/j.foodchem.2018.01.052.
[2] SZAKIEL A, PACZKOWSKI C, KOIVUNIEMI H, et al. Comparison of the triterpenoid content of berries and leaves of lingonberry Vaccinium vitis-idaea from Finland and Poland[J]. Journal of Agricultural and Food Chemistry, 2012, 60(19): 4994-5002. DOI: 10.1021/jf300375b.
[3] EK S, KARTIMO H, MATTILA S, et al. Characterization of phenolic compounds from lingonberry(Vaccinium vitis-idaea)[J]. Journal of Agricultural and Food Chemistry, 2006, 54(26): 9834-9842. DOI: 10.1021/jf0623687.
[4] KURKIN V A, RYAZANOVA T K, PLATONOV I A, et al. Determination of arbutin in Vaccinium vitis-idaea L. leaves[J]. Pharmaceutical Chemistry Journal, 2017, 51(4):281-284. DOI:10.1007/s11094-017-1598-z.
[5] FAN Z L, WANG Z Y, LIU J R. Cold-field fruit extracts exert different antioxidant and antiproliferative activities in vitro[J]. Food Chemistry, 2011, 129(2): 402-407. DOI:http://dx.doi.org/10.1016/j.foodchem.2011.04.091.
[6] ISAAK C K, PETKAU J C, O K, et al. Manitoba lingonberry(Vaccinium vitis-idaea) bioactivities in ischemia-reperfusion injury[J]. Journal of Agricultural and Food Chemistry, 2015, 63(23): 5660-5669. DOI:10.1021/acs.jafc.5b00797.
[7] ALY E M, ALI M A. Effects of bilberry on deoxyribonucleic acid damage and oxidant-antioxidant balance in the lens, induced by ultraviolet radiation[J]. The Malaysian Journal of Medical Sciences, 2014, 21(1): 11.
[8] 樊梓鸾,王振宇.红豆越橘体外抗氧化和抗细胞增殖活性研究[J].现代食品科技,2010,26(10):1081-1086. DOI:10.13982/j.mfst.1673-9078.2010.10.032. FAN Z L, WANG Z Y. Antioxidant and antiproliferative activities of lingonberries in vitro[J]. Modern Food Science and Technology,2010,26(10):1081-1086.
[9] KIVIMAKI A S, SILTARI A, EHLERS P I, et al. Lingonberry juice lowers blood pressure of spontaneously hypertensive rats(SHR)[J]. Journal of Functional Foods, 2013, 5(3): 1432-1440. DOI: 10.1016/j.jff.2013.05.012.
[10] TORRONEN R, KOLEHMAINEN M, SARKKINEN E, et al. Postprandial glucose, insulin, and free fatty acid responses to sucrose consumed with blackcurrants and lingonberries in healthy women[J]. The American Journal of Clinical Nutrition, 2012, 96(3): 527-533. DOI:10.3945/ajcn.112.042184.
[11] ZAMORA-ROS R, KNAZE V, LUJAN-BARROSO L, et al. Estimation of the intake of anthocyanidins and their food sources in the European Prospective Investigation into Cancer and Nutrition(EPIC)study[J]. British Journal of Nutrition, 2011, 106(7): 1090-1099. DOI: 10.1017/S0007114511001437.
[12] GUTIERREZ R M P. Anti-inflammatory effect of birsonimadiol from seeds of Byrsonima crassifolia[J]. Food Science and Biotechnology, 2016, 25(2): 561-566. DOI: 10.1007/s10068-016-0078-7.
[12] AYATOLLAHI A M, ZAREI S M, MEMARNEJADIAN A, et al. Triterpene constituents of Euphorbia erythradenia Bioss. and their anti-HIV activity[J]. Iranian Journal of Pharmaceutical Research, 2016, 15: 19-27.
[14] PAGNING A L N, LATEEF M, TAPONDJOU L A, et al. New triterpene and new flavone glucoside from Rhynchospora corymbosa(Cyperaceae)with their antimicrobial, tyrosinase and butyrylcholinesterase inhibitory activities[J]. Phytochemistry Letters, 2016, 16: 121-128. DOI: http://dx.doi.org/10.1016/j.phytol.2016.03.011.
[15] SUDHAHAR V, KUMAR S A, SUDHARSAN P T, et al. Protective effect of lupeol and its ester on cardiac abnormalities in experimental hypercholesterolemia[J]. Vascular Pharmacology, 2007, 46(6): 412-418. DOI: 10.1016/j.vph.2006.12.005.
[16] JANG H J, HAN I H, KIM Y J, et al. Anticarcinogenic effects of products of heat-processed ginsenoside Re, a major constituent of ginseng berry, on human gastric cancer cells[J]. Journal of Agricultural and Food Chemistry, 2014, 62(13): 2830-2836. DOI: 10.1021/jf5000776.
[17] 朱安运,夏嫱,李小波,等.栀子与西红花中西红花苷纯化、分析及构效关系研究进展[J].药物分析杂志,2018,38(05):735-747. DOI:10.16155/j.0254-1793.2018.05.01. ZHU A Y, XIA Q, LI X B, et al. Research progress of purification,determination and structure-activity relationship of crocins from gardenia and saffron [J]. Chin J Pharm Anal, 2018,38(5):735-747.
[18] 赵瑜, 陈波, 罗旭彪,等. 大孔吸附树脂分离纯化人参二醇类和三醇类皂甙[J]. 天然产物研究与开发,2004,16(3):235-238. DOI:10.16333/j.1001-6880.2004.03.017. ZHAO Y, CHEN B, LUO X B, et al. Study on separation and purification process of panaxdiol type ginsenosides and panaxtriol type ginsenosides with Macroporous adsorption resin[J]. Natural Product Resarch and Development,2004,16(3):235-238.
[19] JIA G, LU X. Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins[J]. Journal of Chromatography A, 2008, 1193(1): 136-141. DOI:http://dx.doi.org/10.1016/j.chroma.2008.04.024.
[20] KUMARI A, KAKKAR P. Lupeol protects against acetaminophen-induced oxidative stress and cell death in rat primary hepatocytes[J]. Food and Chemical Toxicology, 2012, 50(5): 1781-1789. DOI: 10.1016/j.fct.2012.02.042.
[21] JAMUNA S, KARTHIKA K, PAULSAMY S, et al. Confertin and scopoletin from leaf and root extracts of Hypochaeris radicata have anti-inflammatory and antioxidant activities[J].Industrial Crops and Products,2015,70:221-230.DOI:10.1016/j.indcrop.2015.03.039.
[22] SAMEE H, LI Z, LIN H, et al. Anti-allergic effects of ethanol extracts from brown seaweeds[J]. Journal of Zhejiang University Science B, 2009, 10(2): 147-153. DOI: 10.1631/jzus.B0820185.
[23] BOUGANDOURA A, D’ABROSCA B, AMEDDAH S, et al. Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf.(Orobanchaceae)extract[J]. Fitoterapia, 2016, 109: 248-253. DOI: 10.1016/j.fitote.2016.01.010.
[24] 沈思, 李孚杰, 梅光明,等. 茯苓皮三萜类物质含量的测定及其抑菌活性的研究[J]. 食品科学, 2009,30(1):95-98.. SHEN S, LI F J, MEI G M, et al. Study on content detemrination and antibacterial ecffets of trietprenes from seleortia of Poria cocos[J]. Food Science, 2009,30(1):95-98.
[25] 张银堂, 张彦峥, 陈芳, 等. 化学计量学-荧光光谱法研究贝诺酯和双氯芬酸钠与牛血清白蛋白的相互作用[J]. 分析化学, 2009, 37(A01): 220-220. ZHANG Y T, ZHANG Y Z, CHEN F, et al. Chemometrics- fluorescence spectroscopy study of the interaction between benoxidate and diclofenac sodium and bovine serum albumin[J]. Analytical Chemistry, 2009, 37(A01): 220-220.
[26] 马涛, 林峰, 林单, 等. 食源性低聚肽抗过敏活性研究[J]. 食品科技, 2014, 39(2): 27-30.. MA T, LIN F, LIN D, et al. Antiallergic activity of food-derived oligopeptides[J]. Food Science and Technology, 2014, 39(2): 27-30.
[27] 王维有, 曹晨晨, 欧赟, 等. 大枣中环磷酸腺苷的提取及体外抗过敏活性研究[J]. 食品工业科技, 2013, 34(11): 49-52. DOI:10.13386/j.issn1002-0306.2013.11.069. WANG W Y,CAO C C, OU Y, et al. Extraction of cyclic adenosine monophosphate and antiallergic activity from jujube in vitro[J].Food Industry Technology, 2013, 34(11): 49-52.
PDF(1542724 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉