Drought resistance analysis of a V-ATPase c subunit(JrVHAc4)gene from Juglans regia

doi:10.3969/j.issn.1000-2006.201811014

Drought resistance analysis of a V-ATPase c subunit(JrVHAc4)gene from Juglans regia

LI Dapei,WANG Yi ,ZHANG Shangkun ,ZHAO Xiang^1,2,ZHAO Huanyuan ,LIU Yumei^1,2,YANG Guiyan^{1,2 *}

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (02) : 79-85.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (02) : 79-85. DOI: 10.3969/j.issn.1000-2006.201811014

Drought resistance analysis of a V-ATPase c subunit(JrVHAc4)gene from Juglans regia

LI Dapei^1,2,WANG Yi ¹,ZHANG Shangkun ¹,ZHAO Xiang^1,2,ZHAO Huanyuan ^1,2,LIU Yumei^1,2,YANG Guiyan^{1,2 *}

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Abstract

【Objective】 V-ATPase is an important enzyme in plant stress response. This study is under stunll the functional mechanism of V-ATPase subunits in response to stress in walnuts. 【Method】 A V-ATPase c subunit gene(named JrVHAc4)was cloned from the ‘Xiangling’ walnut transcriptome. The stress response function was predicted by analyzing its promoter and expression under drought stress. Meanwhile, JrVHAc4 was inserted into a yeast expression vector pYES2 and transformed into yeast INVSC1 to study its drought resistance function. 【Result】 The open reading frame(ORF)of JrVHAc4 was 495 bp in length, the deduced protein was 16 527.61 u and contained 164 amino acids, whose theoretical isoelectric point(pI)was 8.62. An upstream 1 047 bp promoter segment was identified and found to contain abundant cis-elements, among which some are related to drought response, such as MYC, DOF and MYB. JrVHAc4 was significantly induced in leaves and roots by drought stress, and the induction in leaves and roots were different. Compared with control yeast, we found that expression of JrVHAc4 increased the growth activity of transgenic yeast significantly under different concentrations of mannitol stress. 【Conclusion】 JrVHAc4 can respond to drought stress and improve the drought resistance of transgenic yeasts, JrVHAc4 can be used as an important candidate gene for walnut resistance.

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LI Dapei,WANG Yi ,ZHANG Shangkun ,ZHAO Xiang^1,2,ZHAO Huanyuan ,LIU Yumei^1,2,YANG Guiyan^{1,2 *}. Drought resistance analysis of a V-ATPase c subunit(JrVHAc4)gene from Juglans regia[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(02): 79-85 https://doi.org/10.3969/j.issn.1000-2006.201811014

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