Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz

SHE Lulu, WANG Weijuan, SONG Jingjing, LU Cunfu, CHEN Yuzhen

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (5) : 181-186.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (5) : 181-186. DOI: 10.3969/j.issn.1000-2006.201811029

Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz

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Abstract

【Objective】 This study aimed to elucidate the mechanisms of physiological and biochemical adaptations of alpine plants to cold acclimation. 【Method】 The callus of Tibet Saussurea laniceps Hand.-Mazz. was selected as the plant material to assess changes in antioxidant enzymes and antioxidants during the process of cold acclimation (4 ℃/0 ℃, day/night, dark, for 0, 3, 6, 9, 12 days, respectively). 【Result】 During the period of cold acclimation, freezing tolerance peaked on day 9, with the lethal temperature (LT50) from -3.5 ℃ to -12.0 ℃. After 4 ℃/0 ℃ cold acclimation, soluble protein, proline content and antioxidant enzyme activity increased significantly, reaching maximum levels on day 9, on which protein content increased by 89.1%, proline content was increased 2.14-fold, and superoxide dismutase(SOD), catalase(CAT) and peroxidase(POD) activity increased by 48.04%, 47.28% and 73.18%, respectively. Furthermore, these results were confirmed by three antioxidant enzyme isozyme zymograms. Cold acclimation increased the content of polyphenols, which were increased 3.91-fold compared with the control on day 12. The polyphenol oxidase(PPO) activity increased by 91.35% compared with the control, and these results were consistent with those for the isozyme. 【Conclusion】 The freezing tolerance ofS. laniceps was significantly increased after cold acclimation, showing a unique adaptation mechanism to the low temperature environment. This study established a feasible experimental system that is valid and referenced for studying endangered plants.

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Saussurea laniceps / callus / cold-hardening / freezing tolerance / antioxidant enzyme / antioxidant substance

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SHE Lulu , WANG Weijuan , SONG Jingjing , et al . Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(5): 181-186 https://doi.org/10.3969/j.issn.1000-2006.201811029

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