Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz

doi:10.3969/j.issn.1000-2006.201811029

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (5): 181-186.doi: 10.3969/j.issn.1000-2006.201811029

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Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz

SHE Lulu¹(), WANG Weijuan²(), SONG Jingjing³, LU Cunfu¹, CHEN Yuzhen¹^,^*()

1. The Key Laboratory of Educatin Ministry for Genetic Breeding and Gene Engineering of Woody and Ornamental Plants, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2. Rural Development Center of Beijing Municipal Commission of Science and Technology, Beijing 100195, China
3. Key Enterprise Laboratory for Large-Scale Medicinal Plant Cell Culture, Tianjin Acelbio Co.Ltd., Tianjin 300457, China

Received:2018-11-20 Revised:2019-04-03 Online:2019-10-08 Published:2019-10-08
Contact: CHEN Yuzhen E-mail:465902123@qq.com;113098126@qq.com;chenyuzhen@bfu.edu.cn

Abstract

Abstract:

【Objective】 This study aimed to elucidate the mechanisms of physiological and biochemical adaptations of alpine plants to cold acclimation. 【Method】 The callus of Tibet Saussurea laniceps Hand.-Mazz. was selected as the plant material to assess changes in antioxidant enzymes and antioxidants during the process of cold acclimation (4 ℃/0 ℃, day/night, dark, for 0, 3, 6, 9, 12 days, respectively). 【Result】 During the period of cold acclimation, freezing tolerance peaked on day 9, with the lethal temperature (LT50) from -3.5 ℃ to -12.0 ℃. After 4 ℃/0 ℃ cold acclimation, soluble protein, proline content and antioxidant enzyme activity increased significantly, reaching maximum levels on day 9, on which protein content increased by 89.1%, proline content was increased 2.14-fold, and superoxide dismutase(SOD), catalase(CAT) and peroxidase(POD) activity increased by 48.04%, 47.28% and 73.18%, respectively. Furthermore, these results were confirmed by three antioxidant enzyme isozyme zymograms. Cold acclimation increased the content of polyphenols, which were increased 3.91-fold compared with the control on day 12. The polyphenol oxidase(PPO) activity increased by 91.35% compared with the control, and these results were consistent with those for the isozyme. 【Conclusion】 The freezing tolerance ofS. laniceps was significantly increased after cold acclimation, showing a unique adaptation mechanism to the low temperature environment. This study established a feasible experimental system that is valid and referenced for studying endangered plants.

Key words: Saussurea laniceps, callus, cold-hardening, freezing tolerance, antioxidant enzyme, antioxidant substance

CLC Number:

Q945

SHE Lulu, WANG Weijuan, SONG Jingjing, LU Cunfu, CHEN Yuzhen. Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(5): 181-186.

Figures/Tables 2

References 31

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Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz

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