
Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)
SUN Lina, ZHOU Qun, HUANG Meiping, ZHOU Changpin, XU Shisong, YANG Shuting, DU Ling, GAN Siming, TANG Qing
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6) : 60-66.
Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)
【Objective】 Find a reliable molecular marker to identifyBougainvillea cultivars that are difficult to distinguish, due to cultivar richness and cultivar homonymy and synonymy. 【Method】 A total of 15 simple sequence repeats (SSR) markers were used to amplify DNA samples from 131 Bougainvillea cultivars. SSR amplicons were detected by a capillary electrophoresis method. Cultivar polymorphism and genetic distance were calculated; they were used for clustering analysis and fingerprint construction of all the 131 cultivars.【Result】A total of 85 different alleles were collectively generated by the 15 SSR loci, averaging 5.60 alleles per locus. Shannon’s information index, observed heterozygosity value, and expected heterozygosity value ranged from 0.38 to 1.53, 0.11 to 0.94 and 0.16 to 0.73, with an average of 1.04, 0.50 and 0.57 per locus, respectively. The polymorphism information content ranged from 0.15 to 0.69, with an average of 0.51 per locus. The genetic distance between cultivars ranged from 0.00 to 0.65, with an average of 0.33. Clustering analysis showed that most of the cultivars within a species tended to fall in the same cluster, but some cultivars of the same, whereas certain cultivars from different species were grouped in the same cluster. Several pairs of cultivars were either homonyms or synonyms. Only 11 SSR loci were shown to be effective for identifying the 131 cultivars; thus, the molecular fingerprints of all these cultivars were constructed based on allelic combinations of the 11 markers. 【Conclusion】Further research should be conducted to verify either the Chinese or Latin names of some suspicious cultivars. SSR-based fingerprints ofBougainvillea cultivars constitute a reliable technique for cultivar identification, registration, and intellectual property protection as well as for cultivar clarification in production practices.
Bougainvillea / cultivar classitication / SSR / fingerprints / genetic relationship
[1] |
周群. 三角梅的品种分类[M]. 北京: 金盾出版社, 2009.
|
[2] |
周群. 中国叶子花属植物种质资源及其繁殖技术研究[J]. 中国农学通报, 2008, 24(12):321-324.
|
[3] |
周群, 黄克福, 丁印龙, 等. 中国引栽三角梅属观赏品种的调查与分类鉴定[J]. 江西农业学报, 2011, 23(5):53-56. DOI: 10.19386/j.cnki.jxnyxb.2011.05.017.
|
[4] |
陈瑞阳, 宋文芹, 李秀兰, 等. 中国主要经济植物基因组染色体图谱(第三册): 中国园林花卉植物染色体图谱[M]. 北京: 科学出版社, 2003.
|
[5] |
孙利娜, 王华新, 龚建英, 等. 宝巾花辐射诱变效应研究初报[J]. 北方园艺, 2013(19):91-93.
|
[6] |
|
[7] |
|
[8] |
程本义, 夏俊辉, 龚俊义, 等. SSR 荧光标记毛细管电泳检测法在水稻 DNA 指纹鉴定中的应用[J]. 中国水稻科学, 2011, 25(6):672-676. DOI: 10.3969/j.issn.1001-7216.2011.06.016.
|
[9] |
高源, 刘凤之, 王昆, 等. 基于TP-M13-SSR 指纹图谱的中国原产苹果属植物分子身份证的建立[J]. 植物遗传资源学报, 2015, 16(6):1290-1297. DOI: 10.13430/j.cnki.jpgr.2015.06.022.
|
[10] |
毛秀红, 郑勇奇, 孙百友, 等. 基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建[J]. 林业科学, 2017, 53(10):80-89. DOI: 10.11707/j.1001-7488.20171009.
|
[11] |
段一凡, 王贤荣, 梁丽丽, 等. 桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报(自然科学版), 2014, 38(S1):1-6. DOI: 10.3969/j.issn.1000-2006.2014.S1.001.
|
[12] |
王龙, 薛华柏, 杨健, 等. 80个梨品种SSR特征指纹数据表的构建[J]. 果树学报, 2016, 33(增刊):43-51. DOI: 10.13925/j.cnki.gsxb.2016.S.05.
|
[13] |
王童欣,
|
[14] |
|
[15] |
李房英, 黄彦晶, 吴少华. 三角梅种质资源的ISSR分析[J]. 热带作物学报, 2011, 32(9):1692-1696. DOI: 10.3969/j.issn.1000-2561.2011.09.022.
|
[16] |
孙利娜, 林茂, 陈升侃, 等. 宝巾花基于转录组测序的SSR标记的开发和应用[J/OL]. 分子植物育种, 1-16 [2019- 11- 13]. http://kns.cnki.net/kcms/detail/46.1068.s:20191011.1624.006.html.
|
[17] |
|
[18] |
|
[19] |
|
[20] |
郝晨阳, 王兰芬, 贾继增, 等. SSR 荧光标记和银染技术的比较分析[J]. 作物学报, 2005, 31(2):144-149. DOI: 10.3321/j.issn:0496-3490.2005.02.002.
|
[21] |
李莉, 杨剑波,
|
[22] |
刘新龙, 马丽, 陈学宽, 等. 云南甘蔗自育品种 DNA 指纹身份证构建[J]. 作物学报, 2010, 36(2):202-210. DOI: 10.3724/SP.J.1006.2010.00202.
|
/
〈 |
|
〉 |