Cloning and expression analysis of  BpTCP7 promoter from Betula platyphylla

doi:10.3969/j.issn.1000-2006.20186025

Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla

REN Li,DONG Jingxiang,YANG Yang,HUANG Haijiao,LI Huiyu

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (01) : 32-38.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (01) : 32-38. DOI: 10.3969/j.issn.1000-2006.20186025

Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla

REN Li,DONG Jingxiang,YANG Yang,HUANG Haijiao,LI Huiyu^*

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Abstract

【Objective】BpTCP7 gene, a member of the CIN branch of TCP family, plays an important role in plant growth and development. In order to characterize the function of BpTCP7. 【Method】 A 1 791 bp flanking sequence upstream of the translation initiation codon was cloned, and putative cis-regulatory elements were deciphered from the promoter sequence of BpTCP7 using PLACE and Plant CARE web tools, and transferred into Arabidopsis thaliana. Then, the expression patterns were investigated during salt and drought stress. 【Result】The results showed that cis-regulatory elements involved in cell cycle, flowering, leaf development, multiple hormone-responsiveness, and stress response were included in the promoter region. The Gus reporter gene that was drived the BpTCP7 promoter was detected in all tissues and organs during the reproductive and vegetative phases. Notably, the BpTCP7 promoter was expressed in the leaf margin of young and mature leaves. The expression level of BpTCP7 gene was increased in transgenic A. thaliana after NaCl and polyethylene glycol(PEG)stresses compared with the control.【Conclusion】In conclusion, BpTCP7 participates in biological processes of leaf development, hormone response, drought and salt stress response, and to some extent regulates the development of all tissues and organs during the reproductive and vegetative growth phases.

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REN Li,DONG Jingxiang,YANG Yang,HUANG Haijiao,LI Huiyu. Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(01): 32-38 https://doi.org/10.3969/j.issn.1000-2006.20186025

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