Abstract: 【Objective】The research was conducted to analysis the effects of soil physical and chemical properties on arbuscular mycorrhizal fungi(AMF)and glomalin-related proteins in the rhizosphere soil of afforestation tree species in coastal area of Jiangsu Province, which provided a theoretical support for AMF application in afforestation of coastal area.【Method】Eight different tree species [Carya illinoensis (Wangenh.)K. Koch, Populus lasiocarpa Oliv, Metasequoia glyptostroboides Hu et Cheng, Zelkova serrata(Thunb.)Makinoz, Taxodium ‘Zhongshanshan’, Eucommia ulmoides Oliv, Ginkgo biloba Linn, Elaeagnus pungens Thunb.] of Dafeng Forest Farm were selected as objects. We certificated the relationship between AMF and soil factors based on redundancy and path analysis.【Result】 The highest colonization of AMF was C. illinoensis and the lowest was G. biloba; the highest spore density was E. ulmoides and the lowest was G. biloba. The highest content of total glomalin-related soil protein(T-GRSP)was Z. serrata and the lowest was T. ‘Zhongshanshan’; the highest content easily extractable glomalin-related soil protein(EE -GRSP)was P. lasiocarpa and the lowest was G. biloba. There was a negative correlation between AMF colonization and soil total phosphorus content significantly(P<0.01). The T-GRSP was significant and negative correlation with soil pH(P<0.01), positive correlation with available potassium(P<0.01)and organic carbon in soil(P<0.01). EE-GRSP was positive correlation with total sodium(P<0.01)and negative correlation with total phosphorus content(P<0.05). There was no significant relationship between spore density and soil factors. The results of path analysis showed that soil total phosphorus had the strongest direct effect on AMF colonization rate and soil total potassium, soil conductivity was followed. Soil available potassium had the strongest direct effect on T-GRSP content, and soil pH, soluble organic carbon, total sodium, conductivity were followed; the nitrate nitrogen on T-GRSP content was showed the indirect effect. The direct role of total sodium on EE-GRSP was the strongest, the conductivity and total phosphorus were the second. And the indirect effect on EE-GRSP was total potassium.【Conclusion】AMF colonization was detected in 8 tree species and soil factors had a significant effect on the mutual symbiotic relationship between AMF and tree species.