Effects of gibberellin on early growth and fruiting of potted Betula platyphylla clones

doi:10.3969/j.issn.1000-2006.201902029

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (5): 16-22.doi: 10.3969/j.issn.1000-2006.201902029

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Effects of gibberellin on early growth and fruiting of potted Betula platyphylla clones

WANG Chu(), GU Chenrui, JIANG Jing, LIU Guifeng^*()

State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University), Harbin 150040, China

Received:2019-02-27 Revised:2019-09-08 Online:2019-10-08 Published:2019-10-08
Contact: LIU Guifeng E-mail:wangchu1226@163.com;liuguifeng@126.com

Abstract

Abstract:

【Objective】 This study aimed to identify the best gibberellin treatment to increase the growth and fruiting ofBetula platyphylla. 【Method】 In this experiment, a birch clone was used as the research object and the ramets were treated with GA₃ and GA₄₊₇ at concentrations of 0.0, 0.2, 0.4 and 0.6 g/L. The test lasted for 3 years. In the second year, the rhythmicity of growth height was investigated. In the third year, the growth height, breast diameter, number of infructescences, fresh weight of infructescences and dry weight of seeds under each treatment were investigated. 【Result】 Analysis of variances of growth height, diameter at breast height, and fruit-bearing amount showed that the differences in growth height and fruit-bearing amount were significantly higher at different concentrations of GA₃ and GA₄₊₇(P < 0.01). Both GA ₃ and GA₄₊₇ can promote the growth and fruiting of B. platyphylla; however, the effect of GA₄₊₇ is better. The optimal concentration was determined to be 0.4 g/L by comprehensive evaluation of growth height and fruit-bearing amount. Under this treatment, the growth height 13.69% was higher than that under the control, and the number of infructescences, fresh weight of infructescences, and dry weight of seeds increased by 203.6%, 186.8% and 187.2%, respectively. The growth height curve of B. platyphylla under each treatment accords with the “S” curve model. The fitting coefficients of the logistic equation under each treatment were all higher than 0.99. According to the parameters of the tree height growth model, the reason that gibberellin promotes the growth of B. platyphylla is mainly due to the prolongation of the fast growth period. 【Conclusion】 Both GA₄₊₇ and GA₃ promoted the growth and fruiting of B. platyphylla. GA₄₊₇ was more efficacious than GA₃. GA₄₊₇ at 0.4 g/L is the best concentration and hormone to promote the growth of B. platyphylla.

Key words: gibberellin, Betula platyphylla, early growth, fruiting, tree height

CLC Number:

S792.153

WANG Chu, GU Chenrui, JIANG Jing, LIU Guifeng. Effects of gibberellin on early growth and fruiting of potted Betula platyphylla clones[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(5): 16-22.

Figures/Tables 6

Table 1

Table 2

Table 3

Table 4

Fig.1

Table 5

References 27

[1]	蔡斌华, 庄维兵, 高志红, 等. 赤霉素解除木本植物季节性休眠机制的研究进展[J]. 西北植物学报, 2014, 34(10):2145-2152. DOI: 10.7606/j.issn.1000-4025.2014.10.2145.
	CAI B H, ZHUANG W B, GAO Z H, et al. Research advances on the mechanism of gibberellins releasing seasonal dormancy in woody plants[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(10):2145-2152.
[2]	张达, 王军虹, 王豫颖, 等. 矮化大豆突变体的GA₃调控[J]. 大豆科学, 2008, 27(3):456-460.
	ZHANG D, WANG J H, WANG Y Y, et al. GA₃ regulation in the dwarfing mutant soybean [J]. Soybean Science, 2008, 27(3):456-460.
[3]	李葳, 朱保葛, 徐民新, 等. 矮杆和半矮杆大豆突变体植株生长对外源GA₃的响应[J]. 作物学报, 2008, 34(7):1240-1246. DOI: 10.3724/SP.J.1006.2008.01240.
	LI W, ZHU B G, XU M X, et al. Responses of plant growth of dwarf andsemi-dwarf soybean mutants to exogenous GA₃ [J]. Acta Agronomica Sinica, 2008, 34(7):1240-1246.
[4]	陈显. 外源赤霉素对油茶成花调控机理的研究[D]. 长沙: 中南林业科技大学, 2013.
	CHEN X. The study of flowering mechanism of Camellia oleifera regulated by exogenous gibberellin[D]. Changsha: Central South University of Forestry & Technology, 2013.
[5]	刘芳, 袁华招, 沈欣杰, 等. 外源GA₃和PP₃₃₃对甜樱桃新梢生长及赤霉素代谢关键基因表达的影响[J]. 核农学报, 2013, 27(3):272-278.
	LIU F, YUAN H Z, SHEN X J, et al. Effects of GA₃ And PP₃₃₃ on shoot growth and gene expression of gibberellins metabolism in Prunus avium [J]. Journal of Nuclear Agricultural Sciences, 2013, 27(3):272-278.
[6]	沈惠娟. 赤霉素结构与功能及其在木本植物花发端中的应用[J]. 南京林业大学学报, 1989, 13(2):89-95.
	SHEN H J. Gibberellin structure & function & practical applications in the flower bud initiation of woody plant[J]. Journal of Nanjing Forestry University, 1989, 13(2):89-95.
[7]	杨在琨. 矮化密植早结实的种子园[J]. 林业科技通讯, 1983(10):28-29.DOI: 10.13456/j.cnki.lykt.1983.10.014.
	YANG Z K. Dwarf densely planted early seed garden[J]. Forest Science and Technology, 1983(10):28-29.
[8]	TROMP J, 李培华. 不同赤霉素对苹果花芽形成的影响[J]. 国外农学(果树), 1984(2):5-7.
	TROMP J, LI P H. Effects of different gibberellins on flower bud formation in apple[J]. Foreign Agronomy (Fruit Trees), 1984(2):5-7.
[9]	王洪梅, 周显昌, 周志军, 等. 赤霉素促进针叶树开花结实技术的研究进展[J]. 林业科技, 2011, 36(3):11-15. DOI: 10.3969/j.issn.1001-9499.2011.03.004.
	WANG H M, ZHOU X C, ZHOU Z J, et al. Research of progress of gibberellins_4/7 promote flowering and fruiting in the conifers [J]. Forestry Science & Technology, 2011, 36(3):11-15.
[10]	王秋玉, 曲丽娜, 贾洪柏. 白桦天然种群木材纤维性状、微纤丝角和基本密度的变异[J]. 东北林业大学学报, 2007, 35(2):1-3, 6. DOI: 10.3969/j.issn.1000-5382.2007.02.001.
	WANG Q Y, QU L N, JIA H B. Variation of wood fiber characteristics, microfibril angle and basic density of Betula platyphylla in natural populations [J]. Journal of Northeast Forestry University, 2007, 35(2):1-3, 6.
[11]	刘桂丰, 蒋雪彬, 刘吉春, 等. 白桦多点种源试验联合分析[J]. 东北林业大学学报, 1999, 27(5):9-14. DOI: 10.3969/j.issn.1000-5382.1999.05.003.
	LIU G F, JIANG X B, LIU J C, et al. Study on the joint analysis of provenances of Betula platyphylla [J]. Journal of Northeast Forestry University, 1999, 27(5):9-14.
[12]	杨传平, 刘桂丰, 魏志刚, 等. 白桦强化促进提早开花结实技术的研究[J]. 林业科学, 2004, 40(6):75-78. DOI: 10.3321/j.issn:1001-7488.2004.06.013.
	YANG C P, LIU G F, WEI Z G, et al. Study on intensive breeding technique of accelerating Betula platyphylla flowering and seeding early [J]. Scientia Silvae Sinicae, 2004, 40(6):75-78.
[13]	李开隆, 姜静, 姜莹, 等. 白桦5×5完全双列杂交种苗性状的遗传效应分析[J]. 北京林业大学学报, 2006, 28(4):82-87. DOI: 10.3321/j.issn:1000-1522.2006.04.016.
	LI K L, JIANG J, JIANG Y, et al. Analysis of the genetic effects of seed and seedling traits of Betula platyphylla in a 5 × 5 complete diallel cross design [J]. Journal of Beijing Forestry University, 2006, 28(4):82-87.
[14]	刘宇, 徐焕文, 尚福强, 等. 3个地点白桦种源试验生长稳定性分析[J]. 北京林业大学学报, 2016, 38(5):50-57. DOI: 10.13332/j.1000-1522.20150463.
	LIU Y, XU H W, SHANG F Q, et al. Growth stability of Betula platyphylla provenances from three sites [J]. Journal of Beijing Forestry University, 2016, 38(5):50-57.
[15]	刘宇, 徐焕文, 尚福强, 等. 16年生白桦种源变异及区划[J]. 林业科学, 2016, 52(9):48-56. DOI: 10.11707/j.1001-7488.20160906.
	LIU Y, XU H W, SHANG F Q, et al. Variation and zoning of 16-year-old Betula platyphylla provenance [J]. Scientia Silvae Sinicae, 2016, 52(9):48-56.
[16]	姜静, 杨光, 祝泽兵, 等. 白桦强化种子园优良家系选择[J]. 东北林业大学学报, 2011, 39(1):1-4. DOI: 10.3969/j.issn.1000-5382.2011.01.001.
	JIANG J, YANG G, ZHU Z B, et al. Family selection from intensive seed orchard of Betula platyphylla [J]. Journal of Northeast Forestry University, 2011, 39(1):1-4.
[17]	殷东生, 沈海龙. 林木大年结实特征及其影响因素研究进展[J]. 生态学杂志, 2016, 35(2):542-550. DOI: 10.13292/j.1000-4890.201602.026.
	YIN D S, SHEN H L. Research review on the characteristics of mast seeding in forest trees and its influencing factors[J]. Chinese Journal of Ecology, 2016, 35(2):542-550.
[18]	BONNET-MASIMBERT M. Effect of growth regulators, girdling, and mulching on flowering of young European and Japanese larches under field conditions[J]. Canadian Journal of Forest Research, 1982, 12(2):270-279. DOI: 10.1139/x82-040. doi: 10.1139/x82-040
[19]	ZHAO P, FAN J F, ZHANG S X, et al. Effects of gibberellin A_4/7, 6-benzylaminopurine and chlormequat chloride on the number of male and female strobili and immature cones in Chinese pine (Pinus tabuliformis) with foliar sprays[J]. Journal of Forestry Research, 2011, 22(3):353-359. DOI: 10.1007/s11676-011-0179-3. doi: 10.1007/s11676-011-0179-3
[20]	KONG L S, VON ADERKAS P, ZAHARIA L I. Effects of stem-injected gibberellins and 6-benzylaminopurine on phytohormone profiles and cone yield in two lodgepole pine genotypes[J]. Trees, 2018, 32(3):765-775. DOI: 10.1007/s00468-018-1670-7. doi: 10.1007/s00468-018-1670-7
[21]	HARRISON D L S, OWENS J N. Gibberellin A_4/7 enhanced cone production in Tsuga heterophylla: the influence of gibberellin A_4/7 on seed-and pollen-cone production[J]. International Journal of Plant Sciences, 1992, 153(2):171-177. DOI: 10.1086/297019. doi: 10.1086/297019
[22]	ALMQVIST C. Timing of GA_4/7 application and the flowering of Pinus sylvestris grafts in the greenhouse[J]. Tree Physiology, 2003, 23(6):413-418. DOI: 10.1093/treephys/23.6.413. doi: 10.1093/treephys/23.6.413
[23]	刘雪梅, 杨传平. 东北地区白桦雌配子体的形成与胚胎发育研究[J]. 植物学通报, 2005, 22(2):147-152. DOI: 10.3969/j.issn.1674-3466.2005.02.003.
	LIU X M, YANG C P. Megagametophyte and embryo development of Betula platyphylla in northeastern China [J]. Chinese Bulletin of Botany, 2005, 22(2):147-152.
[24]	刘雪梅, 杨传平. 白桦雌雄花发育周期的时序特征[J]. 林业科学, 2006, 42(12):28-32, 175. DOI: 10.3321/j.issn:1001-7488.2006.12.005.
	LIU X M, YANG C P. Temporal characteristics of developmental cycles of female and male flowers in Betula platyphylla in northeastern China [J]. Scientia Silvae Sinicae, 2006, 42(12):28-32, 175.
[25]	郭军, 马铁民, 李保国, 等. 植物生长调节剂处理对梨矮化砧木SPRB₁₅快速成苗影响的研究[J]. 河北林果研究, 2008, 23(2):176-178. DOI: 10.3969/j.issn.1007-4961.2008.02.018.
	GUO J, MA T M, LI B G, et al. Effects of plant growth regulators on rapid growth seedlings of pear stunted rootstock SPRB₁₅ [J]. Hebei Journal of Forestry and Orchard Research, 2008, 23(2):176-178.
[26]	赵艳, 谭浩, 陈丽梅. 叶片喷施甲醇和乙醇对油菜生长及光合特性的影响[J]. 扬州大学学报(农业与生命科学版), 2013, 34(3):39-43. DOI: 10.16872/j.cnki.1671-4652.2013.03.010.
	ZHAO Y, TAN H, CHEN L M. Comparison of the effects of foliar application of methanol and ethanol on the growth and photosynthetic characteristics in Brassica napus L [J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2013, 34(3):39-43.
[27]	于雪松. 甲醇和乙醇的喷施对蚕豆光合作用和气孔的影响及其分子机理的研究[D]. 昆明: 昆明理工大学, 2013.
	YU X S. The study on effects of methanol and ethanol stimulation on the photosynthesis and stomatal of faba bean[D]. Kunming: Kunming University of Science and Technology, 2013.

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性状 traits	差异来源 source of difference	离差平方和 SS	自由度 df	均方 MS	方差分量 variance component	方差分量比例/% percentage of variance component	F	P
树高/cm growth height	组间	145 064.243	8	18 133.030	988.150	74.05	52.357^**	<0.001
	组内	54 374.462	155	346.334	346.334	25.95
	总数	199 438.705	163		1 334.484	100.00
胸径/cm diameter of height	组间	1.670	8	0.209	0.004	3.20	1.597	0.130
	组内	20.522	155	0.131	0.131	96.80
	总数	22.192	163		0.135	100.00
单株果序数量/个 numbers of infructescence per ramet	组间	207 897.275	8	25 987.159	1 056.929	13.18	3.732^**	0.001
	组内	1 009 552.309	155	6 962.430	6 962.430	86.82
	总数	1 217 449.584	163		8 019.359	100.00
单株果序鲜质量/g fresh weight of infructescence per ramet	组间	79 064.434	8	9 883.054	384.445	11.48	3.335^**	0.002
	组内	429 640.755	155	2 963.040	2 963.040	88.52
	总数	508 705.189	163		3 347.485	100.00
单株种子干质量/g dry weight of seeds per ramet	组间	4 330.217	8	541.277	21.450	12.14	3.488^**	0.001
	组内	22 501.828	155	155.185	155.185	87.86
	总数	26 832.045	163		176.635	100.00

处理 treatment	树高/cm growth height	单株果序数量 number of infructescence per ramet	单株果序鲜质量/g fresh weight of infructescence per ramet	单株种子干质量/g dry weight of seeds per ramet
T1	491.33±25.32 e	55±8.43 c	38.38±6.75 c	8.38±1.54 c
T2	485.00±20.00 e	70±17.45 c	50.18±12.24 bc	9.68±2.45 c
T3	538.42±15.82 cd	73±14.34 c	48.02±10.11 bc	10.70±2.45 bc
T4	558.61±13.37 ab	167±23.92 a	110.07±17.07 a	24.07±4.19 a
T5	566.25±13.48 a	137±34.37 ab	85.29±21.31 ab	19.27±5.47 ab
T6	494.38±16.72 e	57±9.60 c	42.90±7.76 bc	8.81±1.47 c
T7	529.44±15.52 d	70±18.36 c	43.58±10.94 bc	8.14±2.02 c
T8	547.06±15.72 bc	67±17.63 c	46.69±12.80 bc	8.53±2.56 c
T9	551.88±15.15 b	106±30.60 bc	67.36±16.70 bc	13.15±3.56 bc

处理 treatment	树高实测值/cm growth height measured value	R²	方程参数equation parameter
处理 treatment	树高实测值/cm growth height measured value	R²	a	b	t₀	c
T₁	126.06±23.91 e	0.991 2	34.65	125.02	169	8.33
T₂	139.25±20.75 d	0.992 2	36.91	143.29	171	8.01
T₃	174.82±10.73 c	0.998 6	26.84	177.64	165	6.96
T₄	194.56±10.90 ab	0.999 0	28.64	198.51	170	6.75
T₅	201.50±5.99 a	0.998 6	33.06	211.09	178	6.80
T₆	139.63±20.04 d	0.995 9	35.83	140.60	170	8.61
T₇	185.00±11.92 bc	0.999 2	29.88	193.28	172	6.14
T₈	200.50±11.76 a	0.999 6	31.35	206.56	174	6.67
T₉	203.45±13.34 a	0.999 2	32.44	213.07	179	6.47

处理 treatment	速生点/ d fastest growing day	速生点处生长速度/ (cm·d^-1) growing speed at the fastest growing day	速生期参数fast-growing parameter
处理 treatment	速生点/ d fastest growing day		起始时间/d start date	结束时间/d end date	持续时间/d duration	生长量/ cm growth amount	日生长量/ (cm·d^-1) daily growth amount	生长量占年生长量比值/% growth ratio of the to annual growth
T₁	164 b	1.130	137 a	190 b	53 b	52.245 d	0.986	54.279
T₂	171 ab	1.616	140 a	194 ab	54 b	61.425 d	1.422	55.337
T₃	165 b	1.786	131 ab	190 b	60 ab	73.488 c	1.565	56.848
T₄	170 ab	1.755	133 ab	194 ab	63 a	94.475 ab	1.520	57.096
T₅	169 ab	1.747	135 ab	202 ab	67 a	97.558 ab	1.771	57.370
T₆	170 ab	2.113	139 a	191 b	52 b	58.628 d	1.866	56.512
T₇	170 ab	1.600	130 ab	196 ab	65 a	88.906 b	1.403	56.107
T₈	174 a	1.770	133 ab	198 a	65 a	99.129 a	1.548	56.032
T₉	177 a	1.825	135 ab	202 a	67 a	102.558 a	1.597	56.614

Effects of gibberellin on early growth and fruiting of potted Betula platyphylla clones

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赤霉素 gibberellin	编号 No.	质量浓度/(g·L^-1) concentration	处理株数 sample number
对照(CK)	T1	0.0	16
GA₄₊₇	T2	0.0	17
	T3	0.2	19
	T4	0.4	19
	T5	0.6	19
GA₃	T6	0	18
	T7	0.2	19
	T8	0.4	19
	T9	0.6	18