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Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum
HE Qianqian, PENG Qi, ZONG Xiaozhuo, ZHU Tianhui, LI Shujiang
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (5) : 199-208.
PDF(1975 KB)
PDF(1975 KB)
Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum
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【Objective】 The inactivated protein toxin from Arthrinium phaeospermum(AP-Toxin) was used as the induction factor for the resistance of three Bambusa pervariabilis × Dendrocalamopsis grandis varieties, and the correlations of secretory response with the disease index and resistance were analyzed.【Method】 The upper twigs of healthy one-year-old B. pervariabilis× D. grandis varieties of No. 3, 6, and 8 were inoculated with AP-Toxin by puncturing. The twig samples were collected at four different time points after inoculation. Secretion contents including sugar, proteins, and lipids were determined using the sormy sugar content determination method, biuret method, and alkali droplet method, respectively, with slight modifications. Responses of sugar, protein, and lipid contents after induction with AP-Toxin were assessed.【Result】 After induction with Ap-Toxin, protein, soluble sugar, total sugar, and lipids contents were increased in secretions of all B. pervariabilis × D. grandis varieties. Moreover, the disease index of all varieties increased significantly. The resistance of B. pervariabilis× D. grandis to A. phaeospermum infestation was enhanced. Among the three varieties, the disease index of No.6 was the lowest and its secretory response was the weakest, whereas the disease index of No. 8 was the highest and its secretory response was the strongest. Correlation analyses showed that protein, soluble sugar, total sugar, and lipid contents were significantly and positively correlated with the disease index and resistance, while the reducing sugar content was significantly and negatively correlated with disease resistance.【Conclusion】The resistance of B. pervariabilis × D. grandis varieties to A. phaeospermum could be enhanced using AP-Toxin as the induction factor. The stronger the resistance of the variety, the better the induction effect. Our results lay the theoretical foundation for the application of induced disease resistance for the management of forest pests.
AP-Toxin / induced factor / Bambusa pervariabilis × Dendrocalamopsis grandis / secretory response / Arthrinium phaeospermum
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