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Screening of indigenous phosphate⁃solubilizing bacteria from Liquidambar formosana Hance rhizosphere and its potential applications for improving plant growth
SONG Juan, XU Guofang, ZHAO Xing, YAO Yao, YANG Xuexiang, TANG Ronglin, CUI Jiawang, CHEN Fengmao, REN Jiahong
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (3) : 95-104.
PDF(1698 KB)
PDF(1698 KB)
Screening of indigenous phosphate⁃solubilizing bacteria from Liquidambar formosana Hance rhizosphere and its potential applications for improving plant growth
Isolate and identify efficient phosphate solubilizing bacteria(PSB) from Liquidambar formosana Hance rhizosphere soil and investigate the application in phosphorus release.
the PSB strains were graded based on morphological and physiological-biochemical characteristics, microbial identification system (MIDI), BIOLOG analysis and 16S rRNA identification methods. The effect of these PSB strains (JX7，JX18，JX21 and WH10）on plant growth was evaluated by determining the minor and major mineral contents of L. formosana leaves using inductively-coupled plasma atomic emission spectrometry.
Isolates JX7, JX18, WH10 and JX21 were closely related to Variovorax paradoxus, Bacillus cereus, B. mycoides and B.cereus, respectively. The JX21 showed the highest content of soluble phosphate was (9.35 ± 0.89) mg/L, followed by JX7 [（6.63±0.09) mg/L], WH10[ (6.16 ± 0.12 ) mg/L] and JX18 [(5.32 ± 0.07 ) mg/L], all the maximum phosphate was fermented after 72 h of incubation. Phosphate-solubilizing bacteria (JX7, WH10, JX18 and JX21) selected from L. formosana rhizosphere showed a significantly improvement in shoot height and ground diameter of L. formosana (P < 0.05). As well as, these selected strains promoted L. formosana in taking up mineral elements such as phosphorus, magnesium, calcium and zinc under greenhouse conditions.
The isolates showed good phosphate-solubilizing activity and had potential for use as biofertilizer agents. Further study is needed to evaluate beneficial traits to develop commercial applications.
Liquidambar formosana Hance / plant growth promoting bacteria / plant nutrients / phosphate-solubilizing bacteria / biofertilizer
| 1 | TAKAHASHI S，ANWAR M R.Wheat grain yield，phosphorus uptake and soil phosphorus fraction after 23 years of annual fertilizer application to an Andosol[J].Field Crop Res，2007，101(2)：160-171.DOI:10.1016/j.fcr.2006.11.003. |
| 2 | BUCHER M.Functional biology of plant phosphate uptake at root and mycorrhiza interfaces[J].New Phytol，2007，173(1)：11-26.DOI:10.1111/j.1469-8137.2006.01935.x. |
| 3 | MITTAL V，SINGH O，NAYYAR H，et al.Stimulatory effect of phosphate???solubilizing fungal strains (Aspergillusawamori and Penicilliumcitrinum) on the yield of chickpea (Cicerarietinum L.cv.GPF2)[J].Soil Biol Biochem，2008，40(3)：718-727.DOI:10.1016/j.soilbio.2007.10.008. |
| 4 | WALPOLA B C, YOON M H. Isolation and characterization of phosphate solubilizing bacteria and their co?inoculation efficiency on tomato plant growth and phosphorous uptake[J]. Afr J Microbiol Res，2013, 7:266-275. DOI:10.5897/AJMR12.2282. |
| 5 | VIRUEL E，LUCCA M E，SI?ERIZ F.Plant growth promotion traits of phosphobacteria isolated from Puna，Argentina[J].Arch Microbiol，2011，193(7)：489-496.DOI:10.1007/s00203-011-0692-y. |
| 6 | ULéN B，BECHMANN M，F?LSTER J，et al.Agriculture as a phosphorus source for eutrophication in the north?west European countries，Norway，Sweden，United Kingdom and Ireland：a review[J].Soil Use Manag，2007，23(s1)：5-15.DOI:10.1111/j.1475-2743.2007.00115.x. |
| 7 | SINGH H，REDDY M S.Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils[J].Eur J Soil Biol，2011，47(1)：30-34.DOI:10.1016/j.ejsobi.2010.10.005. |
| 8 | DOBBELAERE S，CROONENBORGHS A，THYS A，et al.Effect of inoculation with wild type Azospirillumbrasilense and A.irakense strains on development and nitrogen uptake of spring wheat and grain maize[J].Biol Fertil Soils，2002，36(4)：284-297.DOI:10.1007/s00374-002-0534-9. |
| 9 | EGAMBERDIYEVA D.Plant???growth???promoting rhizobacteria isolated from a Calcisol in a semi?arid region of Uzbekistan：biochemical characterization and effectiveness[J].J Plant Nutr Soil Sci，2005，168(1)：94-99.DOI:10.1002/jpln.200321283. |
| 10 | REYES I，BERNIER L，ANTOUN H.Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicilliumrugulosum[J].Microb Ecol，2002，44(1)：39-48.DOI:10.1007/s00248-002-1001-8. |
| 11 | ZAIDI A，KHAN M，AMIL M.Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicerarietinum L.)[J].Eur J Agron，2003，19(1)：15-21.DOI:10.1016/s1161-0301(02)00015-1. |
| 12 | HINSINGER P，BRAVIN M，DEVAU N，et al.Soil?root?microbe interactions in the rhizosphere：a key to understanding and predicting nutrient bio availability to plants[J].RC Suelo Nutr Veg，2008，8(5)：39-47.DOI:10.4067/s0718-279120080004 00008. |
| 13 | SABER M S M, KABESH M O. Utilization of biofertilizers in field crop production. II. a comparison study on the effect of biofertilization or sulphur application on yield and nutrient uptake by lentil plants[J]. Eur J Soil Sci, 1990, 30: 415-422. |
| 14 | SUNDARA B，NATARAJAN V，HARI K.Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields[J].Field Crop Res，2002，77(1)：43-49.DOI:10.1016/s0378-4290(02)00048-5. |
| 15 | SHEN J，LI R，ZHANG F，et al.Crop yields，soil fertility and phosphorus fractions in response to long?term fertilization under the rice monoculture system on a calcareous soil[J].Field Crop Res，2004，86(2/3)：225-238.DOI:10.1016/j.fcr.2003.08.013. |
| 16 | NURAINI Y，ARFARITA N，SISWANTO B.Isolation and characteristic of nitrogen???fixing bacteria and phosphate???solubilizing bacteria from soil high in mercury in tailings and compost areas of artisanal gold mine[J].Agrivita J Agr Sci，2015，37(1)：0126-0537.DOI:10.17503/agrivita-2015-37-1-p001-007. |
| 17 | ILLMER P，SCHINNER F.Solubilization of inorganic calcium phosphates：solubilization mechanisms[J].Soil Biol Biochem，1995，27(3)：257-263.DOI:10.1016/0038-0717(94)00190-c. |
| 18 | BASHAN Y，KAMNEV A A，DE??BASHAN L E.A proposal for isolating and testing phosphate???solubilizing bacteria that enhance plant growth[J].Biology and Fertility of Soils，2013，49(1)：1-2.DOI:10.1007/s00374-012-0756-4. |
| 19 | KUNITSKY C, OSTERHOUT G, SASSER M. Identification of microorganisms using fatty acid methyl ester (FAME) analysis and the MIDI Sherlock Microbial Identification System［C］// MILLER M. Encyclopedia of rapid microbiological methods. River Grove, IL, USA:DAVIS Healthcare International Publishers: 2005: 1–17. |
| 20 | CHERKAOUI A，HIBBS J，EMONET S，et al.Comparison of two matrix???assisted laser desorption ionization???time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level[J].J Clin Microbiol，2010，48(4)：1169-1175.DOI:10.1128/jcm.01881-09. |
| 21 | NATH D，MAURYA B R，MEENA V S.Documentation of five potassium??and phosphorus??solubilizing bacteria for their K and P???solubilization ability from various minerals[J].Biocatal Agric Biotechnol，2017，10：174-181.DOI:10.1016/j.bcab.2017. 03.007. |
| 22 | JACKSON M L，PRENTICE???HALL I N C，ENGLEWOOD CLIFFS N J. Soil chemical analysis[J].Soil Sci，1958，85(6)：339.DOI:10.1097/00010694-195806000-00014. |
| 23 | KRIEG N R,SNEATH P H A,STALEY J T, et al. Bergy’s manual of determinative bacteriology[J]. Jama?J Am Med Assoc,2000,15(170):408. DOI:10.1136/pgmj.15.170.408. |
| 24 | CHEN X，PIZZATTI C，BONALDI M，et al.Biological control of lettuce drop and host plant colonization by rhizospheric and endophytic streptomycetes[J].Front Microbiol，2016，7：714.DOI:10.3389/fmicb.2016.00714. |
| 25 | YU X，LIU X，ZHU T H，et al.Isolation and characterization of phosphate???solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization[J].Biol Fertil Soils，2011，47(4)：437-446.DOI:10.1007/s00374-011-0548-2. |
| 26 | LI G E，WU X Q，YE J R，et al.Isolation and identification of phytate?degrading rhizobacteria with activity of improving growth of poplar and Masson pine[J].World J Microbiol Biotechnol，2013，29(11)：2181-2193.DOI:10.1007/s11274-013-1384-3. |
| 27 | SLABBINCK B，DE BAETS B，DAWYNDT P，et al.Towards large???scale FAME???based bacterial species identification using machine learning techniques[J].Syst Appl Microbiol，2009，32(3)：163-176.DOI:10.1016/j.syapm.2009.01.003. |
| 28 | DESPRéS V R，NOWOISKY J F，KLOSE M，et al.Characterization of primary biogenic aerosol particles in urban，rural，and high?alpine air by DNA sequence and restriction fragment analysis of ribosomal RNA genes[J].Biogeosciences，2007，4(6)：1127-1141.DOI:10.5194/bg-4-1127-2007. |
| 29 | FYKSE E M，AARSKAUG T，THRANE I，et al.Legionella and non?Legionella bacteria in a biological treatment plant[J].Can J Microbiol，2013，59(2)：102-109.DOI:10.1139/cjm-2012-0166. |
| 30 | ALLEN R A，TOLLEY J O，JESAITIS A J.Preparation and properties of an improved photoaffinity ligand for the N?formyl peptide receptor[J].Biochim Et Biophys Acta BBA?Gen Subj，1986，882(3)：271-280.DOI:10.1016/0304-4165(86)90248-5. |
| 31 | SILINI?CHE H，SILINI A，GHOUL M，et al.Isolation and characterization of plant growth promoting traits of a rhizobacteria：Pantoeaagglomerans lma2[J].Pak J Biol Sci，2012，15(6)：267-276.DOI:10.3923/pjbs.2012.267.276. |
| 32 | HANIF M K，HAMEED S，IMRAN A，et al.Isolation and characterization of a ?2?propeller gene containing phosphobacterium Bacillus subtilis strain KPS?11 for growth promotion of potato (Solanumtuberosum L.)[J].Front Microbiol，2015，6：583.DOI:10.3389/fmicb.2015.00583. |
| 33 | LI X L，LUO L J，YANG J S，et al.Mechanisms for solubilization of various insoluble phosphates and activation of immobilized phosphates in different soils by an efficient and salinity?tolerant Aspergillus Niger strain An2[J].Appl Biochem Biotechnol，2015，175(5)：2755-2768.DOI:10.1007/s12010-014-1465-2. |
| 34 | BERCHEBRU L，RAMEIL P，GAUDIN J C，et al.Normalization of test and evaluation of biothreat detection systems：overcoming microbial air content fluctuations by using a standardized reagent bacterial mixture[J].J Microbiol Methods，2014，105：141-145.DOI:10.1016/j.mimet.2014.07.013. |
| 35 | RODRíGUEZ H，FRAGA R，GONZALEZ T，et al.Genetics of phosphate solubilization and its potential applications for improving plant growth?promoting bacteria[J].Plant and Soil，2006，287(1/2)：15-21.DOI:10.1007/s11104-006-9056-9. |
| 36 | ZHAO K，PENTTINEN P，ZHANG X P，et al.Maize rhizosphere in Sichuan，China，hosts plant growth promoting Burkholderiacepacia with phosphate solubilizing and antifungal abilities[J].Microbiol Res，2014，169(1)：76-82.DOI:10.1016/j.micres.2013.07.003. |
| 37 | SMITH S E, READ D. Mycorrhizal symbiosis[M]. San Diego:USA:Academic Press, 1997. |
| 38 | BHATTACHARYYA D，GARLADINNE M，LEE Y H.Volatile indole produced by rhizobacterium Proteusvulgaris JBLS202 stimulates growth of Arabidopsisthaliana through auxin，cytokinin，and brassinosteroid pathways[J].J Plant Growth Regul，2015，34(1)：158-168.DOI:10.1007/s00344-014-9453-x. |
| 39 | PéREZ?FLORES P L，VALENCIA?CANTERO E，ALTAMIRANO?HERNáNDEZ J，et al.Bacillusmethylotrophicus M4?96 isolated from maize (Zeamays) rhizoplane increases growth and auxin content in Arabidopsisthaliana via emission of volatiles[J].Protoplasma，2017，254(6)：2201-2213.DOI:10.1007/s00709-017-1109-9. |
| 40 | CALVO P，WATTS D B，KLOEPPER J W，et al.Effect of microbial???based inoculants on nutrient concentrations and early root morphology of corn (Zeamays)[J].J Plant Nutr Soil Sci，2017，180(5)：636.DOI:10.1002/jpln.201770055. |
| 41 | RUDRESH D L，SHIVAPRAKASH M K，PRASAD R D.Effect of combined application of Rhizobium，phosphate solubilizing bacterium and Trichoderma spp.on growth，nutrient uptake and yield of chickpea (Ciceraritenium L.)[J].Appl Soil Ecol，2005，28(2)：139-146.DOI:10.1016/j.apsoil.2004.07.005. |
| 42 | GULL M，HAFEEZ F Y，SALEEM M，et al.Phosphorus uptake and growth promotion of chickpea by co???inoculation of mineral phosphate solubilising bacteria and a mixed rhizobial culture[J].Aust J Exp Agric, 2004，44(6)：623.DOI:10.1071/ea02218. |
| 43 | POONGUZHALI S，MADHAIYAN M，TONGMIN S. Isolation and identification of phosphate solubilizing bacteria from Chinese cabbage and their effect on growth and phosphorus utilization of plants[J]. J Microbiol biotechn,2008,18(4): 773-777. DOI: 10.1007/s12275-006-0149-1. |
| 44 | DE FREITAS J R，GERMIDA J J.A root tissue culture system to study winter wheat?rhizobacteria interactions[J].Appl Microbiol Biotechnol，1990，33(5)：589-595.DOI:10.1007/bf00172557. |
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