Effects of GA3 on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’

doi:10.3969/j.issn.1000-2006.201909019

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (3): 26-32.doi: 10.3969/j.issn.1000-2006.201909019

Special Issue: 球根花卉专题

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Effects of GA₃ on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’

JIANG Nannan¹^,²(), ZHANG Qixiang¹, WANG Yuan³, SUN Yin², FANG Yifu²(), XU Jinguang²()

^1.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture of Beijing Forestry University, Beijing, 100083, China
^2.Shandong Academy of Forestry, Jinan 250014, China
^3.Forest Seedling and Flower Station of Shandong Province, Jinan 250014, China

Received:2019-09-09 Revised:2019-10-29 Online:2020-05-30 Published:2020-06-11
Contact: FANG Yifu,XU Jinguang E-mail:abcnnjiang@126.com;fyf7741@163.com;xjg0531@163.com

Abstract

Abstract: Objective

We investigated the effects of gibberellin acid (GA₃) (0, 100, 200, 300 and 1 000 mg/L) on dormancy release to replace low-temperature treatments; furthermore, physiological changes in endogenous hormones and carbohydrate metabolism were examined. The results may provide theoretical and technical support for applied use of GA₃to terminate dormancy and regulate flowering time in Paeonia lactiflora.

Method

We investigated effects of GA₃ at different concentrations on plant growth and flowering using open-field overwintered potted P. lactiflora ‘Da Fugui’ as a control. We measured fructose, glucose and sucrose concentrations using high performance liquid chromatography (HPLC). Activities of enzymes associated with the metabolism of five sugar compounds and content of five endogenous hormones were determined using enzyme-linked immunoassays (ELISA) and liquid chromatography-mass spectrometry(LC-MS), respectively.

Result

The results showed that 100, 200, 300 and 1 000 mg/L GA₃ affected dormancy termination in P. lactiflora ‘Da Fugui’, and substantial differences in plant growth and development were observed in treated seedlings. Stem lodging and wilting rates of seedlings treated with 100, 200, 300 and 1 000 mg/L GA₃ were 0.88%, 18.1%, 65.7% and 100%, respectively. Treatments with 100-300 mg/L GA₃ led to substantially decreased height (variation rate 17.9%-23.5%), number of flowers (variation rate 46.6%-65.9%), and flower diameter (variation rate 11.8%-16.7%); however, the flowering process was normal. During dormancy and during the bud scale bursting period, fructose and glucose were the predominant soluble sugars in ‘Da Fugui’ plants, and the proportion of sucrose increased du?ring bud swelling. During dormancy, bud swelling, and bud scale bursting, sucrose synthase, cell wall invertase, and vacuolar invertase activity increased, whereas sucrose phosphate synthase and alpha-amylase decreased slightly initially and then increased. Gibberellin, auxin, abscisic acid and salicylic acid content initially decreased and then increased, whereas jasmonic acid content showed the opposite pattern.

Conclusion

Treatments with 100-400 mg/L GA₃ affected dormancy release in ‘Da Fugui’ plants, and the strongest effect was observed at 100 mg/L GA₃ which produced the best growth and flowering results. Fructose and glucose are the main soluble sugars during bud dormancy and bud scale burs?ting, and nutrients produced in the roots are transferred to buds in form of sucrose during bud swelling. GA₃ treatments induced considerable changes in the levels of five endogenous hormones, and subsequent synergistic effects elicited the transition from dormancy to seedling growth.

Key words: gibberellin, dormancy release, sugar metabolism, endogenous hormones, Paeonia lactiflora

CLC Number:

S682.1

JIANG Nannan, ZHANG Qixiang, WANG Yuan, SUN Yin, FANG Yifu, XU Jinguang. Effects of GA₃ on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(3): 26-32.

Figures/Tables 6

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Table 1

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环境 environment	GA₃处理水平/ （mg·L^-1） GA₃ concentration	发芽所需时长/d days to sprout	萌芽数 shoot number	地径/mm basal diameter	发芽到开花时长/d days from sprout to flower	折萎率/% wilting rates	株高/cm plant height	成花数 flower amount per pot	花径/cm flower diameter
室内 indoor	100	11	4.72±0.98 c	5.61±0.39 a	75	0.88	39.31±2.95 b	1.83±0.19 b	7.67±1.46 b
	200	10	6.85±1.05 b	5.22±0.65 a	72	21.25	41.86±1.63 b	1.56±0.21 b	8.12±0.68 b
	300	10	7.13±0.85 b	5.17±0.58 a	71	64.47	42.17±3.28 b	1.17±0.16 b	7.85±0.92 b
	1 000	8	8.84±1.37 a	4.45±0.98 b	—	100.00	15.45±1.87 c	—	—
室外 outdoor	0(CK)	151	5.12±1.45 c	6.20±0.86 a	51	0	51.38±0.86 a	3.43±0.33 a	9.21±0.79 a
	100	14	4.95±1.88 c	6.13±1.05 a	—	0.52	12.26±1.03 c	—	—
	200	13	6.92±1.23 b	5.34±0.72 a	—	18.12	15.45±1.24 c	—	—
	300	12	7.24±0.96 b	5.17±0.58 a	—	56.29	18.84±1.37 c	—	—
	1 000	10	8.56±1.24 a	4.56±0.74 b	—	100.00	14.28±0.72 c	—	—

Effects of GA₃ on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’

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