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Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress
CUI Lingjun, LIU Yuxia, LIN Jian, SHI Kaiming
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (4) : 119-124.
PDF(915 KB)
PDF(915 KB)
Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress
The combined effects of arbuscular mycorrhizal fungi (AMF) and salt stress on the morphological structure, endogenous hormone content, and dynamic changes in Phoebe zhennan were studied to provide a theoretical support for P. zhennan afforestation in saline-alkali conditions.
An Epson Perfection V700 dual-lens system was used to scan the full root system. An analysis was performed using Winrhizo software; the mycorrhizal infection rates were determined by the standard method of root segment frequency, and the endogenous hormone content and dynamic changes were determined by enzyme-linked immunosorbent assay (ELISA).
The NaCl concentration was 300 mmol/L, the total length, surface area, volume and number of lateral roots following the AMF treatment were 21.11%, 40.98%, 18.71% and 32.58% higher than those of the non-AMF treatment, respectively; and AMF treatment significantly increased the growth of the root system. With NaCl concentrations increasing, the contents of GA3, IAA and ZR in P. zhennan roots treated with AMF decreased, but the ABA content increased. The NaCl concentration was 300 mmol/L, and the mass ratios of ABA to GA3, ABA to IAA, ABA to ZR, ABA to (GA3 +IAA +ZR) following the AMF treatment were 18.55%, 28.13%, 89.91% and 24.74% lower than those of the non-AMF treatment, respectively; the AMF treatment significantly reduced the root hormone ratio.
In P. zhennan plants under varying degrees of salt stress, AMF treatment increased the total length, surface area, volume and number of lateral roots, altered the hormonal balance, increased the contents of GA3, IAA and ZR, and reduced the content of ABA, indicating that AMF can enhance the salt tolerance of P. zhennan and promote root growth.
Phoebe zhennan / arbuscular mycorrhizal fungi(AMF) / salt stress / endogenous hormone
| 1 | 潘晶，黄翠华，罗君，等. 盐胁迫对植物的影响及AMF提高植物耐盐性的机制[J]. 地球科学进展，2018，33(4)：361-372. |
| 1 | PAN J，HUANG C H，LUO J，et al. Effects of salt stress on plant and the mechanism of arbuscular mycorrhizal fungi enhancing salt tolerance of plants[J]. Adv Earth Sci，2018，33(4)：361-372. DOI:10. 11867 /j. issn. 1001-8166. 2018. 04. 0361. |
| 2 | 郭敏，王楠，付畅. 植物根系耐盐机制的研究进展[J]. 生物技术通报，2012(6)：7-12. |
| 2 | GUO M，WANG N，FU C. Progress of studies on salt tolerance mechanisms in plant root system under salt stress[J]. Biotechnol Bull，2012(6)：7-12. DOI:10. 13560/j. cnki. biotech. bull. 1985. 2012. 06. 026. |
| 3 | 刘咏梅,程聪,姜黎,等.NaCl胁迫下3种柽柳属植物生长、盐离子分布和SOS1基因相对表达量的比较[J].植物资源与环境学报,2019,28(1):1-9. |
| 3 | LIU Y M，CHENG C，JIANG L，et al. Comparisons on growth, salt ion distribution, and relative expression of SOS1 gene in three species of Tamarix Linn. under NaCl stress[J]. Journal of Plant Resources and Environment, 2019,28(1):1-9.DOI:10.3969/j.issn.1674-7895.2019.01.01. |
| 4 | 贺忠群，李焕秀，汤浩茹，等. 丛枝菌根真菌对NaCl胁迫下番茄内源激素的影响[J]. 核农学报，2010，24(5)：1099-1104. |
| 4 | HE Z Q，LI H X，TANG H R，et al. Effect of arbuscular mycorrhizal fungi on tomato endogenous under nacl stress[J]. J Nucl Agric Sci，2010，24(5)：1099-1104. |
| 5 | 宋福强，杨国亭，孟繁荣，等. 丛枝菌根(AM)真菌对大青杨苗木根系的影响[J]. 南京林业大学学报(自然科学版)，2005，29(6)：35-39. |
| 5 | SONG F Q，YANG G T，MENG F R，et al. The effects of arbuscular mycorrhizal fungi on the radicular system of Populusussuriensis seedlings[J]. J Nanjing For Univ （Nat Sci Ed），2005，29(6)：35-39. DOI： 10.3969/j.issn.1000-2006. 2005.06.007. |
| 6 | RUIZ-LOZANO J M，PORCEL R，AZCON C，et al. Regulation by arbuscular mycorrhizae of the integrated physiological response to salinity in plants：new challenges in physiological and molecular studies[J]. J Exp Bot，2012，63(11)：4033-4044. DOI:10. 1093/jxb/ers126. |
| 7 | 黄小辉，陈道静，冯大兰. 不同基质条件下丛枝菌根真菌对桑树生长的影响[J]. 南京林业大学学报(自然科学版)，2019，43(3)：9-16. |
| 7 | HUANG X H，CHEN D J，FENG D L. The effects of arbuscular mycorrhiza fungi on the growth of mulberry in different nursery substrates[J]. J Nanjing For Univ （Nat Sci Ed），2019，43(3)：9-16. DOI:10. 3969 /j. issn. 1000-2006. 201810005. |
| 8 | 孙玉芳，宋福强，常伟，等. 盐碱胁迫下AM真菌对沙枣苗木生长和生理的影响[J]. 林业科学，2016，52(6)：18-27. |
| 8 | SUN Y F，SONG F Q，CHANG W，et al. Effect of arbuscular mycorrhizal fungi on growth and physiology of Elaeagnusangustifolia seedlings subjected to salinity stress[J]. Sci Silvae Sin，2016，52(6)：18-27. DOI:10. 11707 /j. 1001-7488. 20160603. |
| 9 | 唐明. 菌根真菌提高植物耐盐性[M]. 北京：科学出版社，2010. |
| 9 | TANG M. Improvement on salt tolerance of plants by mycorrhizal fungi[M]. Beijing：Science Press，2010. |
| 10 | GARCíA I V，MENDOZA R E. Arbuscular mycorrhizal fungi and plant symbiosis in a saline?sodic soil[J]. Mycorrhiza，2007，17(3)：167-174. DOI:10. 1007/s00572-006-0088-z. |
| 11 | 郑鹏丽，黄晓蓉，费永俊，等. 水分胁迫对桢楠幼树光合生理特性的影响[J]. 中南林业科技大学学报，2019，39(10)：64-70. |
| 11 | ZHENG P L，HUANG X R，FEI Y J，et al. Effects of water stress on photosynthetic physiology characteristics of Phoebezhennan seedlings[J]. J Central South Univ For Technol，2019，39(10)：64-70. DOI:10. 14067/j. cnki. 1673-923x. 2019. 10.010. |
| 12 | 缪勉之. 湖南主要经济树种[M]. 长沙：湖南科学技术出版社，1982. |
| 12 | MIAO M Z. Hunan s main economic tree species [M]. Changsha：Hunan Science & Technology Press，1982. |
| 13 | 肖前辉，敬阳，龚召松. 湖南省楠木天然林质量动态评价[J]. 中南林业科技大学学报，2019，39(10)：7-11. |
| 13 | XIAO Q H，JING Y，GONG Z S. Dynamic evaluation of natural forest quality of Phoebezhennan in Hunan Province[J]. J Central South Univ For Technol，2019，39(10)：7-11. DOI:10. 14067/j. cnki. 1673-923x. 2019. 10. 002. |
| 14 | 杨亚宁，巴雷，白晓楠，等. 一种改进的丛枝菌根染色方法[J]. 生态学报，2010，30(3)：774-779. |
| 14 | YANG Y N，BA L，BAI X N，et al. An improved method to stain arbuscular mycorrhizal fungi in plant roots[J]. Acta Ecol Sin，2010，30(3)：774-779. |
| 15 | 宋会兴，彭远英，钟章成，等. 干旱生境中接种丛枝菌根真菌对三叶鬼针草(Bidens pilosa L.)光合特征的影响[J]. 生态学报，2008，28(8)：3744-3751. |
| 15 | SONG H X，PENG Y Y，ZHONG Z C，et al. Photosynthetic responses of AMF?infected and AMF-free Bidens pilosa L. to drought stress conditions[J]. Acta Ecologica Sinica，2008，28(8)：3744-3751. |
| 16 | 曹岩坡，代鹏，戴素英，等. 丛枝菌根真菌(AMF)对盐胁迫下芦笋幼苗生长及体内Na+、K+、Ca2+、Mg2+含量和分布的影响[J]. 生态学杂志，2015，34(6)：1699-1704. |
| 16 | CAO Y P，DAI P，DAI S Y，et al. Effects of arbuscular mycorrhizal fungi(AMF) on seedling growth and Na+，K+，Ca2+，Mg2+ contents and distribution in Asparagus under salt stress[J]. Chin J Ecol，2015，34(6)：1699-1704. DOI:10. 13292/j. 1000-4890. 2015. 0157. |
| 17 | 马洁怡，王金平，张金池，等. 沿海造林树种根际丛枝菌根真菌与土壤因子的通径分析[J]. 南京林业大学学报(自然科学版)，2019，43(4)：139-147. |
| 17 | MA J Y，WANG J P，ZHANG J C，et al. Path analysis of arbuscular mycorrhizal fungi and soil factors in coastal afforestation tree species[J]. J Nanjing For Univ （Nat Sci Ed），2019，43(4)：139-147. DOI:10. 3969 /j. issn. 1000-2006. 201901012. |
| 18 | CHEN M L，YANG G，SHENG Y，et al. Glomusmosseae inoculation improves the root system architecture，photosynthetic efficiency and flavonoids accumulation of liquorice under nutrient stress[J]. Front Plant Sci，2017，8：931. DOI:10. 3389/fpls. 2017. 00931. |
| 19 | WU Q S，ZOU Y N，HUANG Y M. The arbuscular mycorrhizal fungus Diversisporaspurca ameliorates effects of waterlogging on growth，root system architecture and antioxidant enzyme activities of Citrus seedlings[J]. Fungal Ecol，2013，6(1)：37-43. DOI:10. 1016/j. funeco. 2012. 09. 002. |
| 20 | 舒玉芳，叶娇，潘程远，等. 三峡库区桑树菌根发育特征及菌根对桑苗生长的促进作用[J]. 蚕业科学，2011，37(6)：978-984. |
| 20 | SHU Y F，YE J，PAN C Y，et al. Developmental features of mycorrhiza and its promotion effect on growth of mulberry saplings in Three Gorges reservoir region[J]. Sci Seric，2011，37(6)：978-984. DOI:10. 13441/j. cnki. cykx. 2011. 06. 022. |
| 21 | 汤静. 接种丛枝菌根对柑桔锌素吸收效应的影响研究[D]. 重庆：西南大学，2010. |
| 21 | TANG J. The effect of inoculating of AM fungi on the Zn absorption[D]. Chongqing：Southwest University，2010. |
| 22 | 于振兴. 土壤盐胁迫下植物之间的相互作用及根系和丛枝菌根的影响[D]. 杭州：浙江大学，2015. |
| 22 | YU Z X. Plant?plant interactions and the effects of root systems and arbuscular mycorrhizae under salt stress[D]. Hangzhou：Zhejiang University，2015. |
| 23 | GALVAN-AMPUDIA C S，TESTERINK C. Salt stress signals shape the plant root[J]. Curr Opin Plant Biol，2011，14(3)：296-302. DOI:10. 1016/j. pbi. 2011. 03. 019. |
| 24 | 郭江源，郭伟，毕娜，等. 丛枝菌根真菌对不同含盐量湿地土壤中芦苇生长的影响[J]. 环境科学，2015，36(4)：1481-1488. |
| 24 | GUO J Y，GUO W，BI N，et al. Effects of arbuscular mycorrhizal fungi on the growth of reeds in wetland soils with different salt content[J]. Environ Sci，2015，36(4)：1481-1488. DOI:10. 13227/j. hjkx. 2015. 04. 048. |
| 25 | CRAMER G R，QUARRIE S A. Abscisic acid is correlated with the leaf growth inhibition of four genotypes of maize differing in their response to salinity[J]. Funct Plant Biol，2002，29(1)：111. DOI:10. 1071/pp01131. |
| 26 | MEIXNER C，LUDWIG?MüLLER J，MIERSCH O，et al. Lack of mycorrhizal autoregulation and phytohormonal changes in the supernodulating soybean mutant nts1007[J]. Planta，2005，222(4)：709-715. DOI:10. 1007/s00425-005-0003-4. |
| 27 | 杨海霞，李士美，郭绍霞. 丛枝菌根真菌对紫薇耐盐性的影响[J]. 植物生理学报，2014，50(9)：1379-1386. |
| 27 | YANG H X，LI S M，GUO S X. Effects of arbuscular mycorrhizal fungi on salinity tolerance of Lagerstroemiaindica[J]. Plant Physiol J，2014，50(9)：1379-1386. DOI:10. 13592/j. cnki. ppj. 2014. 0263. |
| 28 | 赵可夫，范海，HARRIS P J C. 盐胁迫下外源ABA对玉米幼苗耐盐性的影响[J]. 植物学报，1995，37(4)：295-300. |
| 28 | ZHAO K F，FAN H，HARRIS P J C. Effect of exogenous aba on the salt tolerance of corn seedlings under salt stress[J]. Acta Bot Sin，1995，37(4)：295-300. |
| 29 | TESTERINK C，MUNNIK T. Phosphatidic acid：a multifunctional stress signaling lipid in plants[J]. Trends Plant Sci，2005，10(8)：368-375. DOI:10. 1016/j. tplants. 2005. 06. 002. |
| 30 | 杨瑞红，刘润进，刘成连，等. AM真菌和水杨酸对草莓耐盐性的影响[J]. 中国农业科学，2009，42(5)：1590-1594. |
| 30 | YANG R H，LIU R J，LIU C L，et al. Effects of arbuscular mycorrhizal fungi and salicylic acid on salt tolerance of strawberry(Fragaria×ananassa Duch.) plants[J]. Sci Agric Sin，2009，42(5)：1590-1594. DOI:10. 3864/j. issn. 0578-1752. 2009. 05. 011. |
| 31 | ZOU Y N，WANG P，LIU C Y，et al. Mycorrhizal trifoliate orange has greater root adaptation of morphology and phytohormones in response to drought stress[J]. Sci Rep，2017，7：41134. DOI:10. 1038/srep41134. |
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