Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress

doi:10.3969/j.issn.1000-2006.201912030

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (4): 119-124.doi: 10.3969/j.issn.1000-2006.201912030

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Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress

CUI Lingjun(), LIU Yuxia, LIN Jian, SHI Kaiming()

Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China

Received:2019-12-18 Revised:2020-03-04 Online:2020-07-22 Published:2021-07-09
Contact: SHI Kaiming E-mail:lingjuncui@163.com;skm331@163.com

Abstract

Abstract: Objective

The combined effects of arbuscular mycorrhizal fungi (AMF) and salt stress on the morphological structure, endogenous hormone content, and dynamic changes in Phoebe zhennan were studied to provide a theoretical support for P. zhennan afforestation in saline-alkali conditions.

Method

An Epson Perfection V700 dual-lens system was used to scan the full root system. An analysis was performed using Winrhizo software; the mycorrhizal infection rates were determined by the standard method of root segment frequency, and the endogenous hormone content and dynamic changes were determined by enzyme-linked immunosorbent assay (ELISA).

Result

The NaCl concentration was 300 mmol/L, the total length, surface area, volume and number of lateral roots following the AMF treatment were 21.11%, 40.98%, 18.71% and 32.58% higher than those of the non-AMF treatment, respectively; and AMF treatment significantly increased the growth of the root system. With NaCl concentrations increasing, the contents of GA₃, IAA and ZR in P. zhennan roots treated with AMF decreased, but the ABA content increased. The NaCl concentration was 300 mmol/L, and the mass ratios of ABA to GA₃, ABA to IAA, ABA to ZR, ABA to (GA₃ +IAA +ZR) following the AMF treatment were 18.55%, 28.13%, 89.91% and 24.74% lower than those of the non-AMF treatment, respectively; the AMF treatment significantly reduced the root hormone ratio.

Conclusion

In P. zhennan plants under varying degrees of salt stress, AMF treatment increased the total length, surface area, volume and number of lateral roots, altered the hormonal balance, increased the contents of GA₃, IAA and ZR, and reduced the content of ABA, indicating that AMF can enhance the salt tolerance of P. zhennan and promote root growth.

Key words: Phoebe zhennan, arbuscular mycorrhizal fungi(AMF), salt stress, endogenous hormone

CLC Number:

S718.43

CUI Lingjun, LIU Yuxia, LIN Jian, SHI Kaiming. Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(4): 119-124.

Figures/Tables 4

Fig. 1

Table 1

Fig. 2

Table 2

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c（NaCl）/ （mmol·L^-1）	总长度/cm length		表面积/cm² surface area		体积/cm³ volume		侧根数 lateral root number
c（NaCl）/ （mmol·L^-1）	+AMF	-AMF	+AMF	-AMF	+AMF	+AMF	+AMF	-AMF
0	248. 4±16.0 a	249. 2±014.0 a	24. 71±1. 36 a	24. 39±1. 26 a	2. 75±0. 21 a	2. 71±0. 41 a	179±11 a	180±14 a
100	227. 5±14.0 b	211. 5±12.0 c	22. 42±1. 51 b	20. 13±1. 45 c	2. 62±0. 32 b	2. 46±0. 26 b	162±15 b	139±13 c
200	207. 4±11.0 c	186. 4±17.0 d	19. 56±1. 47 c	16. 85±1. 88 d	2. 31±0. 20 b	2. 07±0. 32 c	143±8 c	122±24 d
300	148. 6±18.0 e	122. 7±8.0 f	16. 34±1. 33 d	11. 59±1. 65 e	1. 84±0. 16 d	1. 55±0. 24 e	118±7 b	89±11 e

c（NaCl）/ （mmol·L^-1）	ABA/GA₃		ABA/IAA		ABA/ZR		ABA/(GA₃+IAA+ZR)
c（NaCl）/ （mmol·L^-1）	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF
0	1. 32±0. 16 e	1. 35±0. 14 e	2. 47±0. 34 e	2. 49±0. 36 e	9. 79±1. 24 e	9. 92±1. 41 e	0. 79±0. 11 e	0. 80±0. 14 e
100	1. 51±0. 14 b	1. 76±0. 12 c	3. 01±0. 57 d	3. 51±0. 58 c	11. 72±1. 62 d	16. 88±1. 87 c	0. 92±0. 15 e	1. 09±0. 13 d
200	1. 78±0. 22 c	2. 19±0. 17 b	3. 85±0. 42 c	4. 59±1. 08 b	17. 61±1. 83 c	24. 27±2. 67 b	1. 14±0. 24 c	1. 39±0. 24 b
300	2. 48±0. 18 b	2. 94±0. 23 a	4. 76±0. 35 b	6. 10±1. 15 a	22. 14±2. 75 b	42. 05±3. 82 a	1. 52±0. 27 b	1. 89±0. 28 a

Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress

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