Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance

doi:10.3969/j.issn.1000-2006.202003059

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (5): 34-40.doi: 10.3969/j.issn.1000-2006.202003059

Special Issue: 色叶树木与木本花卉专题

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Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance

MA Juanjuan(), ZHAO Bin, CHEN Ying^*(), LING Xichen, YU Jie, CHEN Xi

Co-Innovation Center for the the Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

Received:2020-03-19 Revised:2020-05-15 Online:2020-10-30 Published:2020-10-30
Contact: CHEN Ying E-mail:1597648516@qq.com;chynjfu@163.com

Abstract

Abstract:

【Objective】 Investigating the cold resistance of four Ilex verticillata varieties provides a guidance and a reference for seedling promotion and research of these new varieties. 【Method】The physiological responses of four I. verticillata varieties (‘Red Sprite’, ‘Gray’, ‘Oosterwijk’ and ‘Winter Gold’) to low temperature stress were studied by exposing their branches to temperature treatments of 0 ℃, -7 ℃, -17 ℃ and -27 ℃ for 24 h. Cold resistance of the four varieties was compared. 【Result】The relative conductivity and malondialdehyde content of the four varieties increased with a decrease in temperature, and reached a maximum at -27 ℃. The low temperature semi-lethal temperatures for ‘Red Sprite’, ‘Gray’, ‘Oosterwijk’ and ‘Winter Gold’ were -26.38 ℃, -24.26 ℃, -29.19 ℃ and -20.04 ℃, respectively. Associated with the decrease in treatment temperature, there was an increase in the soluble sugar, soluble protein, and proline contents and osmotic regulators at > -17 ℃ (the soluble sugar of ‘Winter Gold’ reached its highest point at -27 ℃); however, the appearance of peak temperature for each variety was different. The H₂O₂ content and the activity of superoxide dismutase and peroxidase all reached their highest value at -17 ℃. The activity of the catalase enzyme increased sharply at -27 ℃, indicating that the three enzymes had a temperature effect on the scavenging of reactive oxygen species.【Conclusion】The cold resistance of ‘Oosterwijk’ and ‘Gray’ was stronger than that of the other two varieties as a whole. Through the comprehensive analysis of membership function and weight value, the order of cold resistance of the four I. verticillata varieties, from strong to weak was: ‘Oosterwijk’ > ‘Gray’ > ‘Red Sprite’ > ‘Winter Gold’.

Key words: Ilex verticillata varieties, low temperature stress, physiological response, cold resistance evaluation

CLC Number:

S688

MA Juanjuan, ZHAO Bin, CHEN Ying, LING Xichen, YU Jie, CHEN Xi. Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 34-40.

Figures/Tables 6

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品种 varieties	Logistic拟合方程 fitting curve equation of Logistic	半致死温度/℃ LT₅₀	R²
‘Red Sprite’	y=100/(1+1.642e^0.019^x)	-26.4	0.952
‘Gray’	y=100/(1+2.173e^0.032^x)	-24.3	0.969
‘Oosterwijk’	y=100/(1+1.692e^0.018^x)	-29.2	0.976
‘Winter Gold’	y=100/(1+2.140e^0.038^x)	-20.0	0.945

品种 varieties	REC	MDA	H₂O₂	SS	SP	Pro	SOD	POD	CAT	隶属函数平均值 appraisal average value
‘Red Sprite’	0.43	0.87	0.81	0.52	0.39	0.51	0.29	0.17	0.43	0.49
‘Gray’	0.46	0.76	0.70	0.46	0.42	0.67	0.20	0.10	0.44	0.47
‘Oosterwijk’	0.50	0.21	0.75	0.46	0.85	0.65	0.29	0.70	0.32	0.52
‘Winter Gold’	0.42	0.43	0.75	0.44	0.35	0.29	0.15	0.19	0.23	0.36

品种 varieties	抗寒性指数(D) cold resistance index																	综合评价值 comprehensive assessment
品种 varieties	REC	MDA		H₂O₂		SS		SP		Pro		SOD		POD		CAT		综合评价值 comprehensive assessment
‘Red Sprite’	0.034		0.049		0.129		0.038		0.039		0.051		0.010		0.037		0.076	0.549
‘Gray’	0.039		0.020		0.211		0.067		0.040		0.051		0.005		0.008		0.077	0.605
‘Oosterwijk’	0.013		0.020		0.065		0.097		0.117		0.016		0.011		0.025		0.110	0.665
‘Winter Gold’	0.036		0.017		0.146		0.036		0.022		0.042		0.007		0.028		0.045	0.461

Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance

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