Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2009, Vol. 33 ›› Issue (02) : 5.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2009, Vol. 33 ›› Issue (02) : 5. DOI: 10.3969/j.jssn.1000-2006.2009.02.002

Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)

  • ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu
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Abstract

A suppression subtractive hybridization (SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera (Keifer), the leaf was induced for 48 and 72 hours and used as the tester, whereas the induced leaf was employed as driver. Seven hundreds and ten cDNA clones were identified. PCR examination showed that most of the inserted fragments were 200—1 000 bp. The BlastN homology analysis revealed that these genes played varies roles in signal transduction, energy metabolism, response to stress and transportation etc.

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ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu. Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2009, 33(02): 5 https://doi.org/10.3969/j.jssn.1000-2006.2009.02.002

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