Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres

WU Weibing， WANG Mingliang， JING Yi ，DAI Hongqi， ZHOU Xiaofan

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (02) : 83-87.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (02) : 83-87. DOI: 10.3969/j.jssn.1000-2006.2011.02.018

Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres

  • WU Weibing1， WANG Mingliang2， JING Yi1 ，DAI Hongqi1， ZHOU Xiaofan1
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Abstract

A series of fluorescent monodisperse polystyrene microspheres labeled with 1, 8naphthamide dyes were prepared by dispersion copolymerization. Making use of fluorescence resonance energy transfer (FRET), the prepared microspheres showed both the fluorescence of the donor and acceptor dyes under the excitation wavelength of the donor dye. It is found that effective FRET occurs at a relatively low dye concentration and appropriate molar ratio of donor and acceptor. An increase in the dye concentration, which reduces the distance between the donor and acceptor, leads to more effective FRET in microspheres. By changing the dye concentrations, microspheres could show tuned fluorescent signals under a single wavelength excitation. This type of uniform fluorescent microspheres has potential application in bioassays.

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WU Weibing， WANG Mingliang， JING Yi ，DAI Hongqi， ZHOU Xiaofan. Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2011, 35(02): 83-87 https://doi.org/10.3969/j.jssn.1000-2006.2011.02.018

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