Extraction of active components from the fungus Botrytis cinerea and their attraction to the pinewood nematode Bursaphelenchus xylophilus

doi:10.3969/j.jssn.1000-2006.2011.02.033

PAN Cangsang, LONG Ruimin, SHEN Yuemao

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (02) : 147-148.

PDF(1061714 KB)

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2011, Vol. 35 ›› Issue (02) : 147-148. DOI: 10.3969/j.jssn.1000-2006.2011.02.033

Extraction of active components from the fungus Botrytis cinerea and their attraction to the pinewood nematode Bursaphelenchus xylophilus

PAN Cangsang, LONG Ruimin, SHEN Yuemao

Author information +

History +

Abstract

We studied a new way to determine the effect of different factors on Bursaphelenchus. xylophilus migration behavior using sterile sand tray and agar surface as migration matrixes. We also separated different extracts from the fungus Botrytis cinerea culture medium and used a filter paper method to determine the effects of the extracts on B. xylophilus activity. The results showed that pine wiltaffected wood has a strong attraction effect on B. xylophilus. The results differed from the presumption that the attractant is a volatile substance. The inducing force of B. cinerea toward B. xylophilus is stable while the pine skin lixivium has no obvious inducing effect. As well, B. xylophilus was obviously attracted to different fungi (B. cinerea>Pestalotia>Microzyme>control), i.e. the attraction to B. cinerea is greatest. This difference increases as exposure time increases and also as the concentration of metabolic products increase. Also, agar concentration significantly influenced migration behavior with the attractiveness to B. xylophilus increasing as the agar concentration decreases. The studies made to determine the nature of the attractants showed that the relative attractiveness of the different B. cinerea extracts to B. xylophilus differed according to the separation method used. Also, the active substances mainly existed in the extracellular organic phase (ethyl acetate phase) suggesting that they might be ethanol soluble compounds. With increased separation the attraction efficiency decreased. Based on the results, we conclude that the inducing activity depends upon the synergistic action of these extracts. Acknowledgment: This research was supported by National Natural Science Fundation of China (30470234).

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

PAN Cangsang, LONG Ruimin, SHEN Yuemao. Extraction of active components from the fungus Botrytis cinerea and their attraction to the pinewood nematode Bursaphelenchus xylophilus[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2011, 35(02): 147-148 https://doi.org/10.3969/j.jssn.1000-2006.2011.02.033

References

[1]Yang Daojun. Studies on the Growth and Propagation of Bursaphelenchus xylophilus and B. mucronatus in wood and fungi[J]. Forest Research, 1993, 6(4):403-408.
[2]Pan Cangsang. The examining tubes of pine nematodes for early diagnosis and their testing method:China,ZL:02 1 29944.7[P]. 2004.
[3]Zheng Xiaolian, Dong Jingao, Qi Qiusuo, et al. Analysis of toxicity composition and Bioassay of BC toxin from Botrytis cinerea[J]. Acta phytopathologica sinca, 1998, 28(3):269-274.
[4]Zhou Jinyan, Wu Kaiyu, Lei Baoliang, et al. Isolation method and biological activity of metabolites from Botrytis cinerea[J]. Chinese Journal of Applied and Environmental Biology, 2002,8(5):532-34.
[5]Marumo S, Katayama M, Komor E. Microbial production of ABA by B. cinerea[J]. Agricultural and Biological Chemistry, 1982, 46(7):1967-1968.
[6]Ishikawa M, Shuto Y, Watanabe H. βmyrcene, a potent attractant component of pine wood for the pine wood nematode, Bursaphelenchus xylophilus[J].Agric Biol Chem, 1986, 50(7):1863-1866.
[7]Stamps W T, Linit M J. Interaction of intrinsic and extrinsic chemical cues in the behaviour of Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) in relation to its beetle vectors[J].Nematology,2001, 3 (4):295-301.
[8]Collado I G, Hernandez G, Duran R, et al. Metabolites from a shake culture of Botrytis cinerea[J]. Phytochemistry, 1995, 38(3):647-650.
[9]Culter H G, Jacyno J M, Harwood J S, et al. Botcinolide: a biologically active natural product from Botrytis cinerea[J]. Biotechnology Biochemitry,1993,57(11):1980-1982.
[10］Matsumori K, Izumi S, Watanabe H. Hormonelike action of 3octanol and 1octen3ol from Botrytis cinerea on the pine wood nematode,Bursaphelenchus xylophilus[J].Agric Biol Chem, 1989, 53 (7) :1777- 1781.

PDF(1061714 KB)

Accesses

Citation

Detail

Sections

Recommended

The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

〈

〉

Please choose a citation manager

Content to export