Icewater mixture，07 mmol／L cycloheximide and 02 ％ colchicine were used as pretreated reagent for Liriodendron hybrids root tips of testtube plantlet by different time grads. Then the root tips were squashed with Carbol fuchsin and observed with microscope. It had been proved that all these three pretreatments of icewater mixture for 6 h， 07 mmol/L cycloheximide for 2 h， and 02 ％ colchicine for 4 h could obtain metaphase chromosome specimen， but by comparison， root tips pretreated with cycloheximide and colchicine could give much better chromosome morphology. Then Karyotype of the Liriodendron chinense×L.tulipifera was studied with chromosome samples prepared from root tip by 07 mmol/L cycloheximide for 2 h. The result showed that there were 38 chromosome in Liriodendron chinense×L.tulipifera and its karyotype formula was 2n=38=26m+8sm+2st+2t and the karyotypes of Liriodendron chinense×L.tulipifera belong to category 2B of Stebbins.
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