Abstract: This paper gave the sapwood stain fungi on the Tilia miqueliana Maxim, an isolation, culture, identification and staining test. The result showed that Lasiodiplodia and Macrophama came out of the 37% and 22% from the tissue of stain sapwood isolated respectively, tissue of bacteria, other fungi and no any fungi were 41%. Staining test showed that poplar wood stain was caused by the both of fungi. On the basis of morphological characters, the both fungi were identified as Lasiodipladia (Botryodiplodia) theobrumae (Pat. ) Griff Maubl. and Macrophoma nicotinae Sousa da Gamara. The both of was first reported on the Tilia miqueliana Maxim, staining sapwood.