JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2001, Vol. 25 ›› Issue (06): 80-82.doi: 10.3969/j.jssn.1000-2006.2001.06.021

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DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut

SHEN Yong-bao.SHI Ji-sen   

  1. Southern Forest Tree Seed Center of National Forestry Bureau, Nanjing 210037,China
  • Online:2001-12-18 Published:2001-12-18

Abstract: This paper shows that the genomic DNA from different tissues of Ginkgo and Chinese chest- nut was extracted by CTAB method. The results indicate that except for mature leaf, other tissues, uch as dormant bud, young leaf, phloem and half-lignous phloem, can be used to extract DNA easily and successfully. In addition,the value of A260/A280 for each DNA samples above mentioned is over 1. 8,and the quantity is over 22~50 g per 100 mg. Also,clear and bright products of RAPD reveal that the DNA may meet needs of DNA molecular markers.

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