Abstract: The fragment of f3h gene of anthocyanin biosynthesis was amplified by RT-PCR method using total RNA from ripping "Red Ballet" fruit peel as template and oligonucleotides designed from the published sequence of kernel fruits as primers. Sequence analysis showed that this fragment is full-length cDNA of f3h gene and it contained 1 095 base pairs. Sequence comparison revealed that the f3h gene of "Red Ballet" shared homologies of 92%, 83% and 82% with those of "Fuji" apple, Petunia and Arabidopsis isolates., respectively. Then the f3h gene was digested by EcoR V and Bgl Ⅱ endoenzymes and 1 095 bp fragment was recovered. Vector pBI121 and 1 095 bp fragment,were connected using ligase. Reconstituted plasmid pBI12l included the anticipated fragment of f3h gene was obtained and was proved by antibiotic selection and PCR analysis. The reconstituted plasmid is using for the further study.