In vitro plantlet regeneration from seedling explants of Pinus densiflora

ZHU Lihua,, WU Xiaoqin,*, DAI Peipei,, ZHANG Hongyan,

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (02) : 11-14.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (02) : 11-14. DOI: 10.3969/j.jssn.1000-2006.2010.02.003

In vitro plantlet regeneration from seedling explants of Pinus densiflora

  • ZHU Lihua1,2, WU Xiaoqin1,2*, DAI Peipei1,2, ZHANG Hongyan1,2
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Abstract

A plantlet regeneration protocol was developed for P.densiflora by using Cotyledonhypocotyl explants from 21—28 days old aseptically grown seedlings. The explants were cultured on a GD medium containing 4 mg/L 6BA (6benzylaminopurine) and 0.05 mg/L NAA (αnaphthylacetic acid) for 5 weeks to obtain bud induction. Bud growth was enhanced by the elimination of 6BA and the inclusion of 0.1 mg/L NAA in DCR medium. Shoots elongation was achieved on DCR medium supplemented with 0.5—1.0 g/L activated charcoal (AC). Multiple shoots in subcultures were proliferated on GD medium with 2 mg/L BAP and 0.2 mg/L NAA. Root primordia were induced in 68.4% of shoots after 4 weeks of culture on 1/2 GD medium containing 0.2 mg/L NAA. Roots elongated rapidly after the transmission of rooted plantlets to 1/2 GD medium with 0.5 g/L AC. The rooted plantlets were successfully transferred to cups containing a mixture of vermiculiteperlitesand. The surviving rate was approximate 60% after 12 weeks in the greenhouse.

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ZHU Lihua,, WU Xiaoqin,*, DAI Peipei,, ZHANG Hongyan,. In vitro plantlet regeneration from seedling explants of Pinus densiflora[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2010, 34(02): 11-14 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.003

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