In vitro plantlet regeneration from seedling explants of Pinus densiflora

ZHU Lihua,, WU Xiaoqin,*, DAI Peipei,, ZHANG Hongyan,

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2010, Vol. 34 ›› Issue (02) : 11-14.

PDF(942829 KB)
南京林业大学学报（自然科学版）
PDF(942829 KB)
Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2010, Vol. 34 ›› Issue (02) : 11-14. DOI: 10.3969/j.jssn.1000-2006.2010.02.003

In vitro plantlet regeneration from seedling explants of Pinus densiflora

  • ZHU Lihua1,2, WU Xiaoqin1,2*, DAI Peipei1,2, ZHANG Hongyan1,2
Author information +
History +

Abstract

A plantlet regeneration protocol was developed for P.densiflora by using Cotyledonhypocotyl explants from 21—28 days old aseptically grown seedlings. The explants were cultured on a GD medium containing 4 mg/L 6BA (6benzylaminopurine) and 0.05 mg/L NAA (αnaphthylacetic acid) for 5 weeks to obtain bud induction. Bud growth was enhanced by the elimination of 6BA and the inclusion of 0.1 mg/L NAA in DCR medium. Shoots elongation was achieved on DCR medium supplemented with 0.5—1.0 g/L activated charcoal (AC). Multiple shoots in subcultures were proliferated on GD medium with 2 mg/L BAP and 0.2 mg/L NAA. Root primordia were induced in 68.4% of shoots after 4 weeks of culture on 1/2 GD medium containing 0.2 mg/L NAA. Roots elongated rapidly after the transmission of rooted plantlets to 1/2 GD medium with 0.5 g/L AC. The rooted plantlets were successfully transferred to cups containing a mixture of vermiculiteperlitesand. The surviving rate was approximate 60% after 12 weeks in the greenhouse.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
ZHU Lihua,, WU Xiaoqin,*, DAI Peipei,, ZHANG Hongyan,. In vitro plantlet regeneration from seedling explants of Pinus densiflora[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2010, 34(02): 11-14 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.003

References

[1]杨宝君,潘宏阳,汤坚,等. 松材线虫病[M]. 北京:中国林业出版社,2003.
[2]Kishi Y. The Pine Wood Nematode and the Japanese Pine Sawyew[M]. Tokyo: Thomas Company Limited, 1995.
[3]Sommer H E, Brown C L , Kormanik P P. Differentiation of plantlets in longleaf pine (Pinus palustris Mill.) tissue cultured in vitro[J]. Bot Gaz, 1975, 136: 196-200.
[4]朱丽华. 湿地松、火炬松和黑松的组培繁殖技术研究[D]. 南京:南京林业大学,2004.
[5]Isikawa H. Generation of adventitious plant organs by tissue culture methods in forest trees[J]. Bulletin of the Forestry and Forest Products Research Institute, 1987, 343: 119-153.
[6]Choi M S, Lee S G, Park Y G, et al. Micropropagation of Pinus densiflora for erecta uyeki[J]. Korean Journal of Plant Tissue Culture, 1993, 20(6): 315-320.
[7]Taniguchi T. Plant regeneration from somatic embryos in Pinus thunbergii and Pinus densiflora[G]//Morohoshi N, Komamine A. Molecular Breeding of Woody Plants. Amsterdam: Elsevier Science B V, 2001.
[8]Maruyama E, Hosoi Y, Ishii K. Propagation of Japanese red pine (Pinus densiflora Zieb.et Zucc.) via somatic embryogenesis[J]. Prop Ornam Plants, 2005, 5: 199-204.
[9]Shoji M, Sato H, Nakagawa R, et al. Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora[J]. J For Res, 2006, 11: 449-453.
[10]朱丽华,张 艺,吴小芹. 湿地松的组织培养及植株再生[J]. 南京林业大学学报:自然科学版,2004,28(6):47-51.
[11]成小飞,花小梅,李文钿. 马尾松离体培养条件下的微繁殖和菌根的形成[J]. 林业科学研究,1995,8(3):241-246.
[12]唐巍,欧阳藩,郭仲琛. 火炬松成熟合子胚培养直接器官发生和植株再生[J]. 云南植物研究,1997,19(3):285-288.
[13]阕国宁,房建军,葛万川,等. 火炬松、湿地松、晚松组织繁殖的研究[J]. 林业科学研究,1997,10(3):227-232.
[14]李科友,唐德瑞,朱海兰,等. 美国黄松离体胚培养条件下不定芽的形成与根产生的研究[J]. 林业科学,2004,40(4):63-67.
[15]朱丽华,吴小芹. 湿地松成熟合子胚直接器官发生及植株再生[J]. 林业科学,2006,42(8):25-29.
[16]Gonzzalez M V, Rey M, Tavazza R. In vitro adventitious shoot formation on cotyledons of Pinus pinea[J]. Hort Science, 1998, 33(4): 749-750.
[17]Calixto F, Pais S M. Adventitious shoot formation and plant regeneration from Pinus pinaster Sol.Ex Aiton[J]. In vitro Cellular and Development Biology Plant, 1997, 33(2): 119-124.
[18]Kaul K. Plant regeneration from cotyledonhypocotyl explants of Pinus strobus L.[J]. Plant Cell Reports, 1987(6): 5-7.
[19]Burns J A, Schwarz O J,Schlarbaum S E. Multiple shoot production from seedling explants of slash pine (Pinus elliottii E.)[J]. Plant Cell Reports, 1991, 10(9): 439-443.
[20]Kalia R K, Arya S, Kalia S, et al. Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg[J]. Biologia Plantarum, 2007, 51(4): 653-659.
[21]朱丽华,郑丹,吴小芹. 黑松丛生芽的诱导及植株再生[J]. 南京林业大学学报:自然科学版,2006,30(3):27-31.
[22]黄健秋,卫志明. 松属树种的组织培养和原生质体培养[J]. 植物学通报,1994,11(1):34-42.
PDF(942829 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉