Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A

doi:10.3969/j.jssn.1000-2006.2010.02.036

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (02): 151-152.doi: 10.3969/j.jssn.1000-2006.2010.02.036

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Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A

KONG Lingying¹, GUO Daosen¹, ZHAO Boguang², LI Ronggui¹

1.Department of Biology, Qingdao University, Qingdao 266071, China; 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China

Online:2010-05-14 Published:2010-05-14

Abstract

CLC Number:

S763

KONG Lingying, GUO Daosen, ZHAO Boguang, LI Ronggui. Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2010, 34(02): 151-152.

References

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[5]Kawazu K, Kaneko N. Asepsis of the pine wood nematode isolate OKD3 causes it to lose its pathogenicity[J]. Jap J Nematol, 1997, 27(2): 76-80.
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[7]Guo Q, Guo D, Zhao B, et al. Two cyclic dipeptides from Pseudomonas fluorescens GcM51A carried by the pine wood nematode and their toxicities to Japanese black pine suspension cells and seedlings in vitro[J]. J Nematol, 2007, 39(3): 243-247.
[8]Guo D, Zhao B, Li R, et al. Purification of flagellin of Pseudomonas fluorescens GcM51A carried by the pine wood nematote, Bursaphelenchus xylophilus, and its in vitro toxicity to a suspension of cells of Pinus thunbergii[J]. Russian Journal of Nematology, 2008, 16(2): 151-157.
[9]Kersten P J. Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase[J]. Proc Natl Acad Sci USA, 1990, 81: 2280-2284.
[10]Hammel K E, Jensen K A, Mozuch M D, et al. Ligninolysis by a purified lignin peroxidase[J]. J Biol Chen, 1993, 268: 12274-12281.
[11]Hammel K E. Extracellular free radical biochemistry of ligninollytic fungi[J]. New J Chem, 1996, 20: 195-198.
[12]Harvey P, Schoemaker H E, Pslmer J M.Veratryl alcohol as a mediator and the role of radical cation in lignin biodegradation by Phanerochaete chrysosporium[J]. FEBS Letters, 1986, 195: 242-246.
[13]Tien M, Kirk T K. Lignindegrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H202requiring oxygenase[J]. Proc Natl Acad Sci USA, 1984, 81: 2280-2284.
[14]Hartree E F. Determination of protein: a modification of the lowry method that gives a linear photometric response[J]. Analytical Biochemistry, 1972, 48: 422-427.第34卷第2期 2010年3月南京林业大学学报(自然科学版) Journal of Nanjing Forestry University (Natural Science Edition)Vol.34, No.2 Mar., 2010

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Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A

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