Clone and functional prediction of SRNase promoter regions in Chinese pear

doi:10.3969/j.jssn.1000-2006.2010.04.005

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2010, Vol. 34 ›› Issue (04): 21-25.doi: 10.3969/j.jssn.1000-2006.2010.04.005

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Clone and functional prediction of SRNase promoter regions in Chinese pear

WUYUNTana¹,², LIU Xueying¹, LI Zhenguo³

1.The Key Lab of Nonwood Forest Product of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China; 2.School of Forestry Central South University of Forestry and Technology, Changsha 410004, China; 3.Huanggangliang Forest Farm of Keshiketengqi, Chifeng 025350, China

Online:2010-08-06 Published:2010-08-06

Abstract

Abstract: Using TAILPCR, 5′flanking regions of the S13, S12, S21RNase genes with a length of 854 bp, 1 448 bp and 1 137 bp were successfully isolated from ‘Jinhua ’and ‘Maogong’(Pyrus pyrifolia) and ‘Yali’(Pyrus bretschneideri) genomic DNA, the GenBank numbers are HM047239, HM047240 and HM047241. The core promoter regions and some upstream regulatory elements in the three fragments were analyzed using PLACE and PlantCARE software. It is found that all of these genes have the putative TATA box and CAAT box, and the promoters were affected by a variety of conditions as light, ABA, salicylic acid. Alignment analysis for promoter sequences of S13, S12, S21 with 5′flanking sequences of S2, S3, S4, S5 revealed a homologous region of about 200 bp in the upstream sequences of the TATA box in SRNase promoters. Phylogenetic tree constructed by MEG 4.0 suggests that the divergence of SRNase gene was formed before the differentiation of subfamilies.

CLC Number:

S722

WUYUNTana,, LIU Xueying, LI Zhenguo. Clone and functional prediction of SRNase promoter regions in Chinese pear[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2010, 34(04): 21-25.

References

[1]王绪,邓俭英,方锋学,等. ISSR分子标记技术及其在园艺作物中的应用[J]. 广西农业科学,2007,38(4):371-374.
[2]Sato Y, Kurihara A, Ogata T, et al. Mode of selfcompatibility inheritance in Japanese pear[J]. J Jpn Soc Hort Sci, 1988, Supp(2): 76-77.
[3]Liu Y G, Whittier R F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking [J]. Genomics, 1995, 25: 674-681.
[4]Liu Y G, Mitsukawa N, Oosumi T, et al. Efficient isolation and mapping of Arabidopsis thaliana TDNA insert junctions by thermal asymmetric interlaced PCR[J]. Plant, 1995, 8: 457-463.
[5]Tsugeki R, Kochieva E Z, Fedoroff N V. A transposon insertion in the Arabidopsis SSR16 gene causes an embryodefective lethal mutation[J]. The Plant Journal, 1996, 10 (3): 479-489.
[6]乌云塔娜. 中国白梨自交不亲和基因的分离鉴定[D]. 长沙:中南林业科技大学,2003.
[7]Thompson J D, Gibson T J, Plewniak F, et al. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research, 1997, 25: 4876-4882.
[8]Tamura K, Dudley J, Nei M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA)software version 4.0[J]. Molecular Biology and Evolution, 2007: 1596-1599.
[9]Higo K, Ugawa Y, Iwamoto M, et al. Plant cisacting regulatory DNA elements(PLACE) database[J]. Nucl Acids Res, 1999, 27: 297-300.
[10]Lescot M, Déhais P, Thijs G, et al. PlantCARE,a database of plant cisacting regulatory elements and a portal to tools for in silico analysis of promoter sequences [J]. Nucl Acids Res, 2002, 30: 325-327.
[11]Ushijima K, Sassa H, Hirano H. Characterization of the flanking regions of the S RNase genes of Japanese pear(Pyrus serotina) and apple(Malus×domestica)[J]. Gene, 1998, 211: 159-167.
[12]Norioka N, Katayama H, Matsuki T, et al. Sequence comparison of the 5′ flanking regions of Japanese pear(Pyrus pyrifolia Naki)SRNases associated with gametophytic selfincompatibility[J]. Sex Plant, 2001, 13: 289-291.
[13]Dissanayake DMRKK, Norioka S, Norioka N, et al. Cisregulatory elements for pistil specific expression in SRNase promoter region of Japanese pear(Pyrus pyrifolia Nakai)[J]. Acta Hort, 2002, 587: 459-465.
[14]Kaufmann H, Salamini F, Thompson R D. Sequence variability and gene structure at the selfincompatibility locus of Solanum tuberosum[J]. Mol Gen Genet, 1991, 226:4 57 -466.
[15]Coleman C E, Kao Th. The 5′ flanking regions of two Petunia inflata S alleles are heterogeneous and repetitive sequences[J]. Plant Mol Biol, 1992, 18: 499-511.
[16]Chung I K, Lee S Y, Ito T, et al. The 5′ flanking sequences of two S alleles in Lycopersicon peruvianum are highly heterologous but contain short blocks of homologous sequences[J]. Plant Cell Physiol, 1995, 36: 1621-1627.
[17]Matton D P, Mau S L, Okamoto S, et al. The Slocus of Nicotiana alata: genomic organization and sequence analysis of two SRNase alleles[J]. Plant Mol Biol, 1995, 28: 847-858.
[18]Morgan D R, Soltis D E, Robertson K R. Systematic and evolutionary implications of rbcL sequence variation in Rosaceae[J]. Am J Bot, 1994, 81: 890-903.
[19]Prell H. Das problem der unfruchtbarkeit[J]. Nature Wochschr, N F, 1921, 20: 440- 446. (责任编辑郑琰燚)第34卷第4期2010年7月南京林业大学学报(自然科学版)Journal of Nanjing Forestry University (Natural Science Edition)Vol.34, No.4Jul., 2010

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Clone and functional prediction of SRNase promoter regions in Chinese pear

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