Using TAILPCR, 5′flanking regions of the S13, S12, S21RNase genes with a length of 854 bp, 1 448 bp and 1 137 bp were successfully isolated from ‘Jinhua ’and ‘Maogong’(Pyrus pyrifolia) and ‘Yali’(Pyrus bretschneideri) genomic DNA, the GenBank numbers are HM047239, HM047240 and HM047241. The core promoter regions and some upstream regulatory elements in the three fragments were analyzed using PLACE and PlantCARE software. It is found that all of these genes have the putative TATA box and CAAT box, and the promoters were affected by a variety of conditions as light, ABA, salicylic acid. Alignment analysis for promoter sequences of S13, S12, S21 with 5′flanking sequences of S2, S3, S4, S5 revealed a homologous region of about 200 bp in the upstream sequences of the TATA box in SRNase promoters. Phylogenetic tree constructed by MEG 4.0 suggests that the divergence of SRNase gene was formed before the differentiation of subfamilies.
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