Abstract: The conditions for root formation and development in microcuttings of Pinus elliottii which were resistant to Lecanosticta acicola were optimized. The results showed that the addition of 0.4 mg/L PP333 to WPM medium in combination with 4.0 mg/L IBA and 0.07 mg/L NAA was beneficial to root induction phase. After the formation of adventitious root primordia, the microcuttings were transferred into the disk containing vermiculite, which were favorable to root expression. Using this procedure, the frequency of root induction was up to 100 % and the survival rate of regerated plantlets reached up to 85 %. Significant genotypic variation at both the family and the clonewithinfamily levels was observed in the survival rate of transplanted plantlets.