JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (06): 135-139.doi: 10.3969/j.jssn.1000-2006.2012.06.027

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The application prospect of loopmediated isothermal amplification method in forest disease detection

QIU Xiuwen, YE Jianren*, ZHANG Linping   

  1. Jiangsu Key Laboratory for Prevention and Management of Invasive Specie, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2012-11-30 Published:2012-11-30

Abstract: Forest diseases are mainly caused by virus, bacteria, fungi and parasitic organisms, and more than 80 % ofthem are caused by fungi. Loopmediated isothermal amplification (LAMP) is a fast and efficient method with the characteristics of simplicity, facility, and accuracy for DNA amplification. This article was focused on the principles and application of the LAMP technology. It had been widely used in the field of food, clinic, environment, agriculture, industry and animal husbandry. It will have a broad prospect in forestry molecular detection.

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